Protein kinase C is required for the pathogenicity of Setosphaeria turcica on maize

The Septin Gene StSep4 Contributes to the Pathogenicity of Setosphaeria turcica by Regulating the Morphology, Cell Wall Integrity, and Pathogenic Factor Biosynthesis

“…Two week-old maize seedlings (B73) were used for the infection assay. Mycelia were evenly spread onto the upper side of uninjured leaves or the upper side of leaves that had been lightly abraded, as described previously . Inoculated plants were placed in a humidity chamber for 24 h at 25 °C in the dark and then transferred to a growth chamber with a 12 h photoperiod and light provided by fluorescent lamps.…”

“…Mycelia were evenly spread onto the upper side of uninjured leaves or the upper side of leaves that had been lightly abraded, as described previously. 22 Inoculated plants were placed in a humidity chamber for 24 h at 25 °C in the dark and then transferred to a growth chamber with a 12 h photoperiod and light provided by fluorescent lamps. Lesion formation on the top two leaves was examined 14 days after inoculation, and each infection assay was performed with three maize seedlings.…”