Protein Kinase Signaling Pathways in Plant-Colletotrichum Interaction

Jiang, Lingyan; Zhang, Shizi; Su, Jianbin; Peck, Scott C.; Luo, Lijuan

doi:10.3389/fpls.2021.829645

Cited by 10 publications

(6 citation statements)

References 85 publications

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“…We hypothesize that upregulation of such genes could be, partially, due to the initial response from the resistant host, which prevents fungal penetration and rapid establishment of feeding structures. Indeed, MAPK pathways have been reported to play an important role for appressorial formation in Cochliobolus heterostrophus [ 37 ], Colletotrichum orbiculare [ 38 , 39 , 40 ], and Pyrenophora teres [ 41 ]. An MAPK expression is also required for the induction of cellulase-encoding genes and controlling host tissue penetration [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes

Nazarov,

Liu,

Chen

et al. 2024

IJMS

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Rust fungi cause significant damage to wheat production worldwide. In order to mitigate disease impact and improve food security via durable resistance, it is important to understand the molecular basis of host–pathogen interactions. Despite a long history of research and high agricultural importance, still little is known about the interactions between the stripe rust fungus and wheat host on the gene expression level. Here, we present analysis of the molecular interactions between a major wheat pathogen—Puccinia striiformis f. sp. tritici (Pst)—in resistant and susceptible host backgrounds. Using plants with durable nonrace-specific resistance along with fully susceptible ones allowed us to show how gene expression patterns shift in compatible versus incompatible interactions. The pathogen showed significantly greater number and fold changes of overexpressed genes on the resistant host than the susceptible host. Stress-related pathways including MAPK, oxidation–reduction, osmotic stress, and stress granule formation were, almost exclusively, upregulated in the resistant host background, suggesting the requirement of the resistance-countermeasure mechanism facilitated by Pst. In contrast, the susceptible host background allowed for broad overrepresentation of the nutrient uptake pathways. This is the first study focused on the stripe rust pathogen–wheat interactions, on the whole transcriptome level, from the pathogen side. It lays a foundation for the better understanding of the resistant/susceptible hosts versus pathogenic fungus interaction in a broader sense.

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Section: Resultsmentioning

confidence: 99%

Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes

Nazarov,

Liu,

Chen

et al. 2024

IJMS

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“…This demonstrates their roles in mediating crucial signaling pathways for defense against the necrotroph. Protein kinases catalyze reversible phosphorylation and modulate the key processes required for activation of plant defense responses through coordinating signaling networks during pathogen recognition ( Romeis et al., 2001 ; Jiang et al., 2022 ). The cysteine rich receptor-like kinases (CRKs) are a major class of plant receptor like kinases that have been reported to perform key roles in cell death and disease resistance ( Quezada et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling reveals differential defense responses among Alternaria brassicicola resistant Sinapis alba and susceptible Brassica rapa

Ahmed,

Dey,

Senthil-Kumar

et al. 2024

Front. Plant Sci.

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Alternaria blight is a devastating disease that causes significant crop losses in oilseed Brassicas every year. Adoption of conventional breeding to generate disease-resistant varieties has so far been unsuccessful due to the lack of suitable resistant source germplasms of cultivated Brassica spp. A thorough understanding of the molecular basis of resistance, as well as the identification of defense-related genes involved in resistance responses in closely related wild germplasms, would substantially aid in disease management. In the current study, a comparative transcriptome profiling was performed using Illumina based RNA-seq to detect differentially expressed genes (DEGs) specifically modulated in response to Alternaria brassicicola infection in resistant Sinapis alba, a close relative of Brassicas, and the highly susceptible Brassica rapa. The analysis revealed that, at 48 hpi (hours post inoculation), 3396 genes were upregulated and 23239 were downregulated, whereas at 72 hpi, 4023 genes were upregulated and 21116 were downregulated. Furthermore, a large number of defense response genes were detected to be specifically regulated as a result of Alternaria infection. The transcriptome data was validated using qPCR-based expression profiling for selected defense-related DEGs, that revealed significantly higher fold change in gene expression in S. alba when compared to B. rapa. Expression of most of the selected genes was elevated across all the time points under study with significantly higher expression towards the later time point of 72 hpi in the resistant germplasm. S. alba activates a stronger defense response reaction against the disease by deploying an array of genes and transcription factors involved in a wide range of biological processes such as pathogen recognition, signal transduction, cell wall modification, antioxidation, transcription regulation, etc. Overall, the study provides new insights on resistance of S. alba against A. brassicicola, which will aid in devising strategies for breeding resistant varieties of oilseed Brassica.

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“…In Saccharomyces cerevisiae, MAPK cascades are essential for filamentation and invasive growth and regulate cell wall remodelling during the cell cycle and upon different stresses 38 . In Colletotrichum spp., reversible protein phosphorylation by kinases was described to be involved in the regulation of various growth and developmental processes associated with pathogenesis and responding to plant-surface signals 39 . Moreover, in oomycetes protein kinases have been shown to participate in the growth and differentiation of these pathogens.…”

Section: Characterization Of Plasmopara Viticola Secretome and Effectomementioning

confidence: 99%

An in-planta comparative study of Plasmopara viticola proteome reveals different infection strategies towards susceptible and Rpv3-mediated resistance hosts

Figueiredo

Santos

Guerra‐Guimarães

et al. 2022

Sci Rep

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Plasmopara viticola, an obligate biotrophic oomycete, is the causal agent of one of the most harmful grapevine diseases, downy mildew. Within this pathosystem, much information is gathered on the host, as characterization of pathogenicity and infection strategy of a biotrophic pathogen is quite challenging. Molecular insights into P. viticola development and pathogenicity are just beginning to be uncovered, mainly by transcriptomic studies. Plasmopara viticola proteome and secretome were only predicted based on transcriptome data. In this study, we have identified the in-planta proteome of P. viticola during infection of a susceptible (‘Trincadeira’) and a Rpv3-mediated resistance (‘Regent’) grapevine cultivar. Four hundred and twenty P. viticola proteins were identified on a label-free mass spectrometry-based approach of the apoplastic fluid of grapevine leaves. Overall, our study suggests that, in the compatible interaction, P. viticola manipulates salicylic-acid pathway and isoprenoid biosynthesis to enhance plant colonization. Furthermore, during the incompatible interaction, development-associated proteins increased while oxidoreductases protect P. viticola from ROS-associated plant defence mechanism. Up to our knowledge this is the first in-planta proteome characterization of this biotrophic pathogen, thus this study will open new insights into our understanding of this pathogen colonization strategy of both susceptible and Rpv3-mediated resistance grapevine genotypes.

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Protein Kinase Signaling Pathways in Plant-Colletotrichum Interaction

Cited by 10 publications

References 85 publications

Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes

Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes

Comparative transcriptome profiling reveals differential defense responses among Alternaria brassicicola resistant Sinapis alba and susceptible Brassica rapa

An in-planta comparative study of Plasmopara viticola proteome reveals different infection strategies towards susceptible and Rpv3-mediated resistance hosts

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