Protein kinases involved in epidermal barrier formation: The AKT family and other animals

Rogerson, Clare; O'Shaughnessy, R

doi:10.1111/exd.13696

Cited by 9 publications

(6 citation statements)

References 132 publications

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“…Akt activation in keratinocytes promotes epidermal cell differentiation with the increased expression levels of loricrin and filaggrin [26]. Akt activity is also essential for the barrier, especially in the formation of the cornified envelope during the late and terminal differentiation [27]. Akt pathway is generally activated by several extracellular signals such as growth factor, cytokines through phosphoinositide 3-kinase (PI3K)-dependent signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

Jung

Jeong

Cho

et al. 2019

IJMS

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The main function of the skin is to protect the body from the external environment. The barrier function of the skin is mainly provided by the stratum corneum, which consists of corneocytes bound with the corneodesmosomes and lamellar lipids. Skin barrier proteins like loricrin and filaggrin also contribute to the skin barrier function. In various skin diseases, skin barrier dysfunction is a common symptom, and skin irritants like detergents or surfactants could also perturb skin barrier function. Many efforts have been made to develop strategies to improve skin barrier function. Here, we investigated whether the microfluidized lysates of Lactobacillus rhamnosus (LR), one of the most widely used probiotic species for various health benefits, may improve the skin barrier function in a reconstructed human epidermis, Keraskin™. Application of LR lysate on Keraskin™ increased the expression of tight junction proteins; claudin 1 and occludin as determined by immunofluorescence analysis, and skin barrier proteins; loricrin and filaggrin as determined by immunohistochemistry and immunofluorescence analysis and qPCR. Also, the cytotoxicity of a skin irritant, sodium lauryl sulfate (SLS), was alleviated by the pretreatment of LR lysate. The skin barrier protective effects of LR lysate could be further demonstrated by the attenuation of SLS-enhanced dye-penetration. LR lysate also attenuated the destruction of desmosomes after SLS treatment. Collectively, we demonstrated that LR lysate has protective effects on the skin barrier, which could expand the utility of probiotics to skin-moisturization ingredients.

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Section: Discussionmentioning

confidence: 99%

Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

Jung

Jeong

Cho

et al. 2019

IJMS

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“…PI3K-Akt signalling has been proven to have important effects on blood vessel morphogenesis and endothelial dysfunction. Akt is a member of the AGC kinases, and phosphorylation at Ser615 and Ser633 plays a crucial role in multiple cellular and physiological effects including proliferation, migration, cell growth and metabolism [28]. Akt can directly phosphorylate eNOS, thus potentiating the enzyme's catalytic activity in response to a variety of stimuli [29].…”

Section: Discussionmentioning

confidence: 99%

A comparative study unraveling the effects of TNF-α stimulation on endothelial cells between 2D and 3D culture

Wang

Chen²,

Gao

et al. 2020

Biomed. Mater.

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Endothelial cell (EC) dysfunction is an important predictor of and contributor to the pathobiology of cardiovascular diseases. However, most in vitro studies are performed using monolayer cultures of ECs on 2D tissue polystyrene plates (TCPs), which cannot reflect the physiological characteristics of cells in vivo. Here, we used 2D TCPs and a 3D culture model to investigate the effects of dimensionality and cardiovascular risk factors in regulating endothelial dysfunction. Cell morphology, oxidative stress, inflammatory cytokines and endothelial function were investigated in human umbilical vein endothelial cells (HUVECs) cultured in 2D/3D. The differentially expressed genes in 2D/3D-cultured HUVECs were analysed using Enrichr, Cytoscape and STRING services. Finally, we validated the proteins of interest and conﬁrmed their relevance to TNF-α and the culture microenvironment. Compared with 2D TCPs, 3D culture increased TNF-α-stimulated oxidative stress and the inflammatory response and changed the mediators secreted by ECs. In addition, the functional characteristics, important pathways and key proteins were determined by bioinformatics analysis. Furthermore, we found that some key proteins, notably ACE, CD40, Sirt1 and Sirt6, represent a critical link between endothelial dysfunction and dimensionality, and these proteins were screened by bioinformatics analysis and verified by western blotting. Our observations provide insight into the interdependence between endothelial dysfunction and the complex microenvironment, which enhances our understanding of endothelial biology or provides a therapeutic strategy for cardiovascular-related diseases.

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“…Ozone exposure led to activation of the JAK2/STAT3 and AKT1/NF-κB pathways in mouse lung (Mishra et al 2016). In the skin, these pathways are relevant to eczema pathogenesis (Howell et al 2019;Rogerson and O'Shaughnessy 2018) with the JAK/STAT pathway being an important therapeutic target.…”

Section: Introductionmentioning

confidence: 99%

“…O 3 exposure led to the activation of the Jak2/signal transducer and activator of transcription 3 and Akt1/NF-κB pathways in mouse lungs ( Mishra et al., 2016 ). In the skin, these pathways are relevant to eczema pathogenesis ( Howell et al., 2019 ; Rogerson and O’Shaughnessy, 2018 ), with the Jak/signal transducer and activator of transcription pathway being an important therapeutic target.…”

Section: Introductionmentioning

confidence: 99%

Modeling of Temporal Exposure to the Ambient Environment and Eczema Severity

Thomas

Tan²,

Javadzadeh³

et al. 2022

JID Innovations

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Protein kinases involved in epidermal barrier formation: The AKT family and other animals

Cited by 9 publications

References 132 publications

Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

A comparative study unraveling the effects of TNF-α stimulation on endothelial cells between 2D and 3D culture

Modeling of Temporal Exposure to the Ambient Environment and Eczema Severity

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