Protein micro- and macroarrays: digitizing the proteome

Lopez, Mary F.; Pluskal, Malcolm G.

doi:10.1016/s1570-0232(02)00336-7

Cited by 59 publications

(26 citation statements)

References 32 publications

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“…Here, basic but critically important biochemical issues come into play as protein fractions are isolated or enriched. Protein separation or isolation is typically achieved by one of three approaches: "protein chips" or activated surfaces that bind proteins based on chemical characteristics or two-dimensional (2-D) gel electrophoresis; 7,8 such methods are used in other articles in this issue including Butterfield and coworkers 4 and Choe and coworkers. 9 In contrast to these approaches, liquid chromatography (LC) also can be used to separate peptides before direct introduction to tandem mass spectrometry (MS).…”

Section: A Quantitative Proteomic Methods Using Liquid Chromatography-mentioning

confidence: 99%

Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer’s disease

Montine

Woltjer

Pan

et al. 2006

NeuroRX

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Summary: Systems biology offers enormous potential to understand the complexity of human brain aging and neurodegenerative diseases. Proteomics has an important role in these investigations because of its unique strengths and because of the potential central pathogenic contribution of pathological protein to several of these diseases. Here we have reviewed the methods and presented some examples of liquid chromatography-electrospray ionization-tandem mass spectrometrybased proteomics, with and without quantification using isotope-coded affinity tags, in the investigation of aging and Alzheimer's disease. As protocols and methods for improved quantitative high-throughput proteomics constantly improve, this approach will likely continue to provide deeper insight into human brain aging and neurodegenerative diseases.

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Section: A Quantitative Proteomic Methods Using Liquid Chromatography-mentioning

confidence: 99%

Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer’s disease

Montine

Woltjer

Pan

et al. 2006

NeuroRX

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“…The basic approach is very similar to that of microarrays (83)(84)(85). Antibodies or other affinity reagents (e.g., aptamers, peptides) are spotted onto some sort of matrix.…”

Section: Protein Arraysmentioning

confidence: 99%

Proteomics for Protein Expression Profiling in Neuroscience

Freeman

Hemby

2004

Neurochem Res

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As the technology of proteomics moves from a theoretical approach to a practical reality, neuroscientists will have to determine the most appropriate applications for this technology. Neuroscientists will have to surmount difficulties particular to their research, such as limited sample amounts, heterogeneous cellular compositions in samples, and the fact that many proteins of interest are rare, hydrophobic proteins. This review examines protein isolation and protein fractionation and separation using two-dimensional electrophoresis (2-DE) and mass spectrometry proteomic methods. Methods for quantifying relative protein expression between samples (e.g., 2-DIGE, and ICAT) are also described. The coverage of the proteome, ability to detect membrane proteins, resource requirements, and quantitative reliability of different approaches is also discussed. Although there are many challenges in proteomic neuroscience, this field promises many rewards in the future.

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“…Until now, protein/antigen arrays have been established by several groups, differing in surface materials, sensitivities and applications (reviewed in: Lopez and Pluskal, 2003;Seong and Choi, 2003;Templin et al, 2003;Zhu and Snyder, 2003).…”

Section: Protein/antigen Arraysmentioning

confidence: 99%

Proteomic Studies Using Microarrays

Feilner¹,

Kreutzberger²,

Niemann

et al. 2004

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Microarray technology plays an increasing role in proteomic research. We give an overview about recent developments in this technology focusing on molecular interaction studies using protein and antibody microarrays. We report about technical aspects in the development of protein microarrays and describe different surfaces and detection modes. Furthermore, we review the applications of protein microarrays in different molecular interaction studies including interactions of proteins with antibodies, proteins, DNA, small molecules and enzymes. Advantages and limitations of the microarray-based methods with other in vitro methods have been compared. We present the increasing applications of protein and antibody microarrays in basic research, diagnostics, drug discovery, and in vitro-risk assessment of nutrients.

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Protein micro- and macroarrays: digitizing the proteome

Cited by 59 publications

References 32 publications

Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer’s disease

Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer’s disease

Proteomics for Protein Expression Profiling in Neuroscience

Proteomic Studies Using Microarrays

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