Protein Microarrays for Ocular Diseases

Uncovering the Depths of the Human Proteome: Antibody-based Technologies for Ultrasensitive Multiplexed Protein Detection and Quantification

“…Each array is printed with control samples with varying amounts of predetermined protein concentration, to establish a calibration curve and allow for absolute protein quantification ( 51 ). Detection is achieved through colorimetric methods, fluorescence, or chemiluminescence ( 52 ). The analytical sensitivity of the technology is reported to be at the picogram to femtogram levels, with a CV of less than 10% between technical replicates ( 51 , 52 ).…”

“…Detection is achieved through colorimetric methods, fluorescence, or chemiluminescence ( 52 ). The analytical sensitivity of the technology is reported to be at the picogram to femtogram levels, with a CV of less than 10% between technical replicates ( 51 , 52 ). The technology has been extensively employed for proteomics research using cell and tissue lysates, such as for tumor profiling, and demonstrated utility in therapeutic clinical research trials ( 47 , 48 , 53 , 54 ).…”