Protein Microchips: Use for Immunoassay and Enzymatic Reactions

Arenkov, Pavel; Kukhtin, Alexander; Gemmell, Anne; Voloshchuk, S. G.; Chupeeva, V. V.; Mirzabekov, Andrei D.

doi:10.1006/abio.1999.4363

Cited by 508 publications

(332 citation statements)

References 18 publications

(20 reference statements)

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“…[59][60][61][62][63] To design protein microarrays, we used DP as a protein support because the many carboxylic acid moieties on the periphery can effectively immobilize target proteins. DP-coated surfaces can load more proteins and more protein activity than planar surfaces.…”

Section: Dp-immobilized Surfaces For Diagnostic Toolsmentioning

confidence: 99%

Dendrimer porphyrin-based self-assembled nano-devices for biomedical applications

Jeong

Yoon

Jang

2012

Polym J

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Dendrimers have attracted great interest for the design of functional materials. Because the core porphyrin unit in dendrimer porphyrin (DP) is surrounded by large poly(benzyl ether) dendritic wedges with ionic peripheries, the resulting electrostatic interaction with oppositely charged polymeric materials has been used to create various functional nano-devices. In this paper, we briefly review DP-based self-assembled biomedical nano-devices, including DP-loaded polyion complex micelles, the ternary complex systems used for gene delivery, polymer-metal complex micelles, the hollow nanocapsules used for combination cancer therapy, and the DP-immobilized surfaces used for diagnostic tools.

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Section: Dp-immobilized Surfaces For Diagnostic Toolsmentioning

confidence: 99%

Dendrimer porphyrin-based self-assembled nano-devices for biomedical applications

Jeong

Yoon

Jang

2012

Polym J

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“…Bait molecules are printed onto a variety of substrate surfaces, including membranes [26], modified glass surfaces [25], and hydrogels [27]. Proteins and antibodies are generally immobilized via non-covalent adsorption or covalent coupling to amine-reactive surfaces.…”

Section: Forward-phase Protein Microarrays (Fpas)mentioning

confidence: 99%

Array-based proteomics: mapping of protein circuitries for diagnostics, prognostics, and therapy guidance in cancer

et al. 2006

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The human proteome, due to the enormity of post-translational permutations that result in large numbers of isoforms, is much more complex than the genome and alterations in cancer can occur in ways that are not predictable by translational analysis alone. Proteomic analysis therefore represents a more direct way of investigating disease at the individual patient level. Furthermore, since most novel therapeutic targets are proteins, proteomic analysis potentially has a central role in patient care. At the same time, it is becoming clear that mapping entire networks rather than individual markers may be necessary for robust diagnostics as well as tailoring of therapy. Consequently, there is a need for high-throughput multiplexed proteomic techniques, with the capability of scanning multiple cases and analysing large numbers of endpoints. New types of protein arrays combined with advanced bioinformatics are currently being used to identify molecular signatures of individual tumours based on protein pathways and signalling cascades. It is envisaged that analysing the cellular 'circuitry' of ongoing molecular networks will become a powerful clinical tool in patient management.

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“…13 Other approaches array antibodies to specific proteins. [14][15][16][17][18][19][20][21] In some cases, whole tissuederived samples have been directly arrayed on to slides, to assess reactivity of total protein lysates. [22][23][24] A limitation of these methodologies is that proteins undergo numerous posttranslational modifications -phosphorylations, glycosylations, as well as many other modifications -which are critical to their functions, as they can determine activity, stability, localization and turnover.…”

Section: Protein Microarrays As a Tool To Probe The Human Proteomementioning

confidence: 99%

Identification of novel targets for cancer therapy using expression proteomics

2002

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Although most drugs target proteins, the proteome has remained largely untapped for the discovery of drug targets. The sequencing of the human genome has had a tremendous impact on proteomics and has provided a framework for protein identification. There is currently substantial interest in implementing proteomics platforms for drug target discovery. Although the field is still in the early stages, current proteomic tools include a variety of technologies that could be implemented for large-scale protein expression analysis of cells and tissues, leading to discovery of novel drug targets. Proteomics uniquely allows delineation of global changes in protein expression patterns resulting from transcriptional and post-transcriptional control, post-translational modifications and shifts in proteins between different cellular compartments. Some of the current technologies for proteome profiling and the application of proteomics to the analysis of leukemias by our group are reviewed. Leukemia (2002) 16, 478-485.

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Protein Microchips: Use for Immunoassay and Enzymatic Reactions

Cited by 508 publications

References 18 publications

Dendrimer porphyrin-based self-assembled nano-devices for biomedical applications

Dendrimer porphyrin-based self-assembled nano-devices for biomedical applications

Array-based proteomics: mapping of protein circuitries for diagnostics, prognostics, and therapy guidance in cancer

Identification of novel targets for cancer therapy using expression proteomics

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