Protein Misfolding Cyclic Amplification

Moda, Fabio; Pritzkow, Sandra; Soto, Claudio

doi:10.1007/978-1-4614-5305-5_6

Cited by 1 publication

(1 citation statement)

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“…PMCA under properly optimized conditions has been believed to faithfully amplify prions from different species in vitro [ 29 ]. The parental prion seed strain traits have been observed to remain in the PMCA amplified prion products [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Generation of human chronic wasting disease in transgenic mice

Wang

Qin

Camacho

et al. 2021

acta neuropathol commun

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Chronic wasting disease (CWD) is a cervid prion disease caused by the accumulation of an infectious misfolded conformer (PrPSc) of cellular prion protein (PrPC). It has been spreading rapidly in North America and also found in Asia and Europe. Although bovine spongiform encephalopathy (i.e. mad cow disease) is the only animal prion disease known to be zoonotic, the transmissibility of CWD to humans remains uncertain. Here we report the generation of the first CWD-derived infectious human PrPSc by elk CWD PrPSc-seeded conversion of PrPC in normal human brain homogenates using in vitro protein misfolding cyclic amplification (PMCA). Western blotting with human PrP selective antibody confirmed that the PMCA-generated protease-resistant PrPSc was derived from the human PrPC substrate. Two lines of humanized transgenic mice expressing human PrP with either Val or Met at the polymorphic codon 129 developed clinical prion disease following intracerebral inoculation with the PMCA-generated CWD-derived human PrPSc. Diseased mice exhibited distinct PrPSc patterns and neuropathological changes in the brain. Our study, using PMCA and animal bioassays, provides the first evidence that CWD PrPSc can cross the species barrier to convert human PrPC into infectious PrPSc that can produce bona fide prion disease when inoculated into humanized transgenic mice.

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Section: Discussionmentioning

confidence: 99%