Protein modification caused by a high dose of gamma irradiation in cryo-sterilized plasma: Protective effects of ascorbate

Żbikowska, Halina Małgorzata; Nowak, Paweł; Wachowicz, Barbara

doi:10.1016/j.freeradbiomed.2005.09.012

Cited by 44 publications

(28 citation statements)

References 30 publications

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“…The majority of the studies have been on free radical scavengers, which have limited ionizing irradiation damage in proteins in aqueous environments [3,12,28]. Recently, however, Grieb et al [9] reported that a radioprotective cocktail solution, which included mannitol, was successful in protecting mechanical properties of human semitendinosus tendon at 50 kGy under regulated conditions.…”

Section: Discussionmentioning

confidence: 99%

Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging

Seto

Gatt

Dunn

2008

Clin Orthop Relat Res

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Ionizing radiation could supplement tissue bank screening to further reduce the probability of diseases transmitted by allografts if denaturation effects can be minimized. It is important, however, such sterilization procedures be nondetrimental to tissues. We compared crosslinking and free radical scavenging potential methods to accomplish this task in tendon tissue. In addition, two forms of ionizing irradiation, gamma and electron beam (e-beam), were also compared. Crosslinkers included 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and glucose, which were used to add exogenous crosslinks to collagen. Free radical scavengers included mannitol, ascorbate, and riboflavin. Radioprotective effects were assessed through tensile testing and collagenase resistance testing after irradiation at 25 kGy and 50 kGy. Gamma and e-beam irradiation produced similar degenerative effects. Crosslinkers had the highest strength at 50 kGy, EDC treated tendons had 54% and 49% higher strength than untreated, for gamma and e-beam irradiation respectively. Free radical scavengers showed protective effects up to 25 kGy, especially for ascorbate and riboflavin. Crosslinked samples had higher resistance to collagenase and over a wider dose range than scavenger-treated. Of the options studied, the data suggest EDC precrosslinking or glucose treatment provides the best maintenance of native tendon properties after exposure to ionizing irradiation.

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Section: Discussionmentioning

confidence: 99%

Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging

Seto

Gatt

Dunn

2008

Clin Orthop Relat Res

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“…It has been established as a major water-soluble intracellular antioxidant (Asmus et al, 1996). Ascorbate (at 193 K) can readily repair all oxidizing free radicals with greater reduction potentials, which includes hydroxyl radicals (Zbikowska et al, 2006). Because the ascorbyl radical has its unpaired electron in a highly delocalized system, it is a relatively unreactive free radical.…”

Section: Discussionmentioning

confidence: 99%

Radioprotectant screening for cryocrystallography

Southworth-Davies

Garman

2006

J Synchrotron Radiat

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Radiation damage continues to present a problem to crystallographers using cryocooled protein crystals at third-generation synchrotrons. Free-radical scavengers have been suggested as a possible means of reducing the rate of this damage. The screening of a large number of potential radioprotectants was undertaken with an online microspectrophotometer using cystine and cysteine to model protein disulfide bonds and thiol groups, respectively. Oxidizedlipoic acid was tested as a possible model disulfide bond. The evidence for the effectiveness of ascorbate as a radioprotectant was strengthened, and quinone, 2,2,6,6-tetramethyl-4-piperidone, and reduced dithiothreitol showed promise as radioprotectants.

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“…P-OOHs were measured by the guanidine-perchloric acid-ferric-xylenol orange method (G-PCA-FOX) [22,23]. The assay is based on the oxidation of Fe 2+ by peroxides in the presence of the dye xylenol orange that gives a coloured complex with the Fe 3+ generated.…”

Section: Assay Of Protein Hydroperoxidementioning

confidence: 99%

Relation of plasma protein oxidation parameters and paraoxonase activity in the ageing population

2008

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The incidence of atherosclerosis increases with age. Oxidative changes in proteins and lipids are considered to be among the molecular mechanisms leading to endothelial dysfunction. Paraoxonase (PON1) is exclusively associated with high-density lipoprotein (HDL) and protects both HDL and low-density lipoprotein (LDL) from oxidation. PON1 has two cysteine residues for its antioxidant function. We investigated the relation between PON1 activity and protein oxidation parameters such as protein hydroperoxides (P-OOH), protein carbonyl (PCO), total thiol (T-SH) and advanced oxidation protein products (AOPP). Our study also covered other oxidative stress parameters such as oxidised LDL (oxLDL) and superoxide dismutase activity in the plasma of young, middle-aged and elderly individuals. PON1 activity of elderly and middle-aged individuals was decreased significantly compared with that in the young group. oxLDL levels of elderly individuals were increased significantly compared with those of both the young and middle-aged individuals. P-OOH, PCO and AOPP levels of the elderly and middle aged individuals were higher compared with those of the young. On the other hand, T-SH levels of the elderly and middle-aged individuals were lower compared with those of the young. Side by side with the decrease in the T-SH levels in the middle-aged and elderly groups as compared to the young, the increase we have observed in other protein oxidation parameters in the groups leading to decreasing PON1 activity might, we think, create a predisposition to atherosclerosis.

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Protein modification caused by a high dose of gamma irradiation in cryo-sterilized plasma: Protective effects of ascorbate

Cited by 44 publications

References 30 publications

Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging

Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging

Radioprotectant screening for cryocrystallography

Relation of plasma protein oxidation parameters and paraoxonase activity in the ageing population

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