Protein oxidation under extremely low frequency electric field in guinea pigs. Effect of N-acetyl-L-cysteine treatment

Abstract

Abstract. Modern age exposes humans to an increasing level of electromagnetic activity in their environment due to overhead power lines and transformers around residential areas. Studies have shown that treatment with antioxidants can suppress the oxidative damage induced by electromagnetic fields in various frequencies of the non-ionizing radiation band. In this study, we detected protein carbonyl content (PCO), advanced oxidation protein products (AOPP) in liver and 3-nitrotyrosine (3-NT) levels in plasma of guinea pigs in order to investigate the effects of N-acetyl-L-cysteine (NAC) administration on oxidative protein damage induced by power frequency electric (E) field (50 Hz, 12 kV/m, 7 days/ 8 h/day). We also analyzed hepatic hydroxyproline level to study protein synthesis. According to the findings of the present study, no statistically significant changes occurred in PCO, AOPP and 3-NT levels of the guinea pigs that were exposed to the E field with respect to the control group. However, liver hydroxyproline level was significantly diminished in the E field exposure group compared to the control and PCO, hydroxyproline and 3-NT levels changed significantly in the NAC-administrated groups.

Effects of Low Magnetic Irradiation on Morphology and Ultrastructure of Parotid Glands in Rats and Amelioration by Vitamin E

“…These observations were confirmed with statistical analysis of optical density of PAS reaction (Table 1). Similar increase in PAS reaction was observed after EMFs exposure in different tissues [23][24][25][26] and explained by actual mucous transformation indicative of adaptive change taken by serous acini in response to the stressful effect induced by the EMFs with increased secretion of cortisol and accumulation of glycogen in the cells. This finding was confirmed by electron microscope finding of multiple electron lucent secretory granules in ELF-MF exposed group (Figure 3 B).…”

“…These metabolites were injurious to other cells and their release was chemotactic for other cells as neutrophils or to lymphocytes. In addition, oxidative stress stimulates the expression of genes involved in collagen biosynthesis [25].…”

Inhibitory Effects of Sigma-2 Receptor Agonists on T Lymphocyte Activation

“…Whether this effect is occurring by a mechanism involving interference on Ca ++ signaling or calcineurin phosphatase activity as in the case of the well-known NFAT inhibitors CsA and Tacrolimus (Kiani et al, 2000; Martinez-Martinez and Redondo, 2004), remains to be elucidated. Besides, NFAT activation plays a promoting role in cancer (Duque et al, 2005; Viola et al, 2005; Gachet and Ghysdael, 2009). Thus, it is tempting to speculate that some of the effects of σ-2 agonists on cancer may take place though NFAT inhibition.…”

The effect of acetylsalicylic acid as a radioprotector

“…[32] Antioksidanlar son zamanlarda gerek kanser önlenmesinde, gerekse radyasyon gibi toksik ajanların hasarından korunmada giderek ilgi çekmekte ve bu konuda oksidatif/nitrozif stres ve ilişkili mekanizmalara ilişkin bilgi birikimi artmaktadır. [32,38,39] Besin katkısı olarak kullanılanların bazılarının yiyeceklerde kullanılmasının mutajenik, karsinojenik ve toksik etkili olduğu belirtilmiştir. [40] Yeni çalışmalarda, antioksidanların, genellikle mutajenik olmadığı, tersine radyasyon dâhil bazı kimyasalların mutajenik etkisini önleyebildiği gösterilmiştir.…”

“…[40] Yeni çalışmalarda, antioksidanların, genellikle mutajenik olmadığı, tersine radyasyon dâhil bazı kimyasalların mutajenik etkisini önleyebildiği gösterilmiştir. [18,19,38,39] Son zamanlarda aspirinin antioksidan özelliklerini kanıtlayan veriler hızla artmaktadır. [14,16,41] Aspirinin antioksidan özelliğinin, prostaglandin sentezinin SR üretimini arttırması, aspirinin ise prostaglandin sentezini inhibe etmesinden kaynaklandığı öne sürülmektedir.…”