Protein patterns, serotyping and plasmid DNA profiles in the epidemiologic fingerprinting ofPseudomonas aeruginosa

Walia, Sandeep; Madhavan, T.; Williamson, Tom; Kaiser, Alan L.; Tewari, Rupinder

doi:10.1007/bf01963096

Cited by 21 publications

(13 citation statements)

References 25 publications

(5 reference statements)

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“…Whole-cell protein pattern analysis by SDS-PAGE and dendrograms obtained from numerical analysis is one of the techniques used for typing of bacterial species and grouping (Walia et al, 1988). In the present study analysis of whole cell protein from the different isolates using SDS-PAGE indicated that based on banding pattern the isolates can be clubbed into two major groups.…”

Section: T H I S C O P Y I S F O R P E R S O N a L U S E O N L Y -D Imentioning

confidence: 54%

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Sowmya

Sachindra

2016

Polish Journal of Microbiology

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A b s t r a c tCarotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to Flavobacteriaceae family were isolated using kanamycincontaining marine agar and identified using the molecular techniques and their phenotypic characteristics were studied along with their potential to produce carotenoids. Analysis of random amplification of polymorphic DNA (RAPD) banding patterns and the fragment size of the bands indicated that the 10 isolates fall under two major groups. Based on 16S rRNA gene sequence analysis the isolates were identified as Vitellibacter sp. (3 isolates), Formosa sp. (2 isolates) and Arenibacter sp. (5 isolates). Phenotypically, the isolates showed slight variation from the reported species of these three genera of Flavobacteriaceae. Only the isolates belonging to Vitellibacter and Formosa produced flexirubin, a typical yellow orange pigment produced by most of the organisms of the family Flavobacteriaceae. Vitellibacter sp. and Formosa sp. were found to produce higher amount of carotenoids compared to Arenibacter sp. and zeaxanthin was found to be the major carotenoid produced by these two species. The study indicated that Vitellibacter sp. and Formosa sp. can be exploited for production of carotenoids, particularly zeaxanthin. K e y w o r d s: Arenibacter sp., Flavobacteriaceae, Formosa sp., Vitellibacter sp., carotenoid, zeaxanthin

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Section: T H I S C O P Y I S F O R P E R S O N a L U S E O N L Y -D Imentioning

confidence: 54%

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Sowmya

Sachindra

2016

Polish Journal of Microbiology

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“…[19] Different electrophoretic methodologies have been employed in the typing of Clostridium species by protein patterns, and it appears to be the most popular general approach. Typeability is extremely high, and in theory all strains can be typed; in practice, well over 95% of isolates can be typed to previously deÞ ned types and any new patterns can be added as new types.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity Among Toxigenic Clostridia Isolated From Soil, Water, Meat and Associated Polluted Sites in South India

Sathish

Swaminathan

2009

Indian Journal of Medical Microbiology

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“…Another advantage is that the level of reproducibility reported for the SDS-PAGE technique is extremely high and this is not affected by long-term storage of strains [27].…”

Section: Discussionmentioning

confidence: 99%

Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

Costas¹,

Holmes²,

Sloss³

1990

Epidemiol. Infect.

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SUMMARYTwenty-four cultures comprising 20 clinical isolates of 'Klebsiella aerogenes' from two hospitals, a reference strain of 'K. aerogenes' and the type strains of three other Klebsiella species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into 12 protein types. Comparison with established typing methods indicated that the level of discrimination of SDS-PAGE was similar to that achieved with conventional typing methods but the strains were grouped differently. Protein typing subdivided five serotype K3 isolates that could also be distinguished by phage typing. Conversely, three strains of protein type 11 were clearly distinguishable by both serotyping and phage typing. We conclude that high-resolution SDS-PAGE of proteins provides an effective adjunct to other methods for typing isolates of 'K. aerogenes'.

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Protein patterns, serotyping and plasmid DNA profiles in the epidemiologic fingerprinting ofPseudomonas aeruginosa

Cited by 21 publications

References 25 publications

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Genetic Diversity Among Toxigenic Clostridia Isolated From Soil, Water, Meat and Associated Polluted Sites in South India

Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

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