Protein Profiles and Dehydrin Accumulation in Some Soybean Varieties (&amp;lt;i&amp;gt;Glycine max&amp;lt;/i&amp;gt; L. Merr) in Drought Stress Conditions

Arumingtyas, Estri Laras; Savitri, Evika Sandi; Purwoningrahayu, Runik Dyah

doi:10.4236/ajps.2013.41018

Cited by 28 publications

(14 citation statements)

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“…Water deficit stress do led to a series of changes including biochemical alterations like accumulation of osmolytes and specific proteins involved in stress tolerance [ 3 ]. One of the proteins that play a role in the mechanism of drought resistance is the LEA types of protein known as dehydrin [ 4 ]. In cotton production, drought is the main abiotic stress responsible for plant growth compromise and severe yield loss.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton

et al. 2018

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BackgroundLate embryogenesis abundant (LEA) proteins are large groups of hydrophilic proteins with major role in drought and other abiotic stresses tolerance in plants. In-depth study and characterization of LEA protein families have been carried out in other plants, but not in upland cotton. The main aim of this research work was to characterize the late embryogenesis abundant (LEA) protein families and to carry out gene expression analysis to determine their potential role in drought stress tolerance in upland cotton. Increased cotton production in the face of declining precipitation and availability of fresh water for agriculture use is the focus for breeders, cotton being the backbone of textile industries and a cash crop for many countries globally.ResultsIn this work, a total of 242, 136 and 142 LEA genes were identified in G. hirsutum, G. arboreum and G. raimondii respectively. The identified genes were classified into eight groups based on their conserved domain and phylogenetic tree analysis. LEA 2 were the most abundant, this could be attributed to their hydrophobic character. Upland cotton LEA genes have fewer introns and are distributed in all chromosomes. Majority of the duplicated LEA genes were segmental. Syntenic analysis showed that greater percentages of LEA genes are conserved. Segmental gene duplication played a key role in the expansion of LEA genes. Sixty three miRNAs were found to target 89 genes, such as miR164, ghr-miR394 among others. Gene ontology analysis revealed that LEA genes are involved in desiccation and defense responses. Almost all the LEA genes in their promoters contained ABRE, MBS, W-Box and TAC-elements, functionally known to be involved in drought stress and other stress responses. Majority of the LEA genes were involved in secretory pathways. Expression profile analysis indicated that most of the LEA genes were highly expressed in drought tolerant cultivars Gossypium tomentosum as opposed to drought susceptible, G. hirsutum. The tolerant genotypes have a greater ability to modulate genes under drought stress than the more susceptible upland cotton cultivars.ConclusionThe finding provides comprehensive information on LEA genes in upland cotton, G. hirsutum and possible function in plants under drought stress.Electronic supplementary materialThe online version of this article (10.1186/s12863-017-0596-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton

et al. 2018

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“…Acumulación de proteínas LEA (Welin et al, 1994), principalmente las dehidrinas (Arumingtyas et al, 2013); elongación radicular disminuida, reducción del área foliar (Hu y Xiong, 2014); cierre de estomas (Froux et al, 2005).…”

Section: Deficiencia Hídricaunclassified

Mecanismos de respuesta al estrés abiótico: hacia una perspectiva de las especies forestales

Espinoza

Vallejo-Reyna

2019

RMCF

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El estrés de origen abiótico tiene un importante impacto negativo para la productividad y supervivencia de los principales cultivos agrícolas y ecosistemas forestales del mundo. Las tendencias actuales del cambio climático pronostican un aumento en la severidad y frecuencia de fenómenos climáticos y condiciones ambientales adversas como la sequía, salinidad del agua y suelo, así como temperaturas extremas. A lo largo de la evolución, las plantas han desarrollado diversos mecanismos moleculares, morfológicos y fisiológicos para responder a las condiciones desfavorables del entorno. La comprensión de dichos mecanismos es esencial para implementar estrategias de conservación y mejoramiento genético de especies vegetales, a fin de proteger la biodiversidad y producir organismos tolerantes / resistentes al daño ocasionado por el estrés abiótico. Sin embargo, la mayoría de los estudios disponibles sobre identificación, transducción de señales y procesos de respuesta al estrés en plantas deriva de investigaciones en especies anuales. Aunque existen algunos trabajos realizados en taxones forestales de los géneros Populus, Eucalyptus, Picea y Pinus, se carece de estudios dedicados a especies mexicanas. Por su alta riqueza y diversidad biológica, México es un país privilegiado; no obstante, la falta de conocimiento sobre la susceptibilidad de sus recursos forestales es el principal obstáculo para diseñar planes de acción orientados a mitigar las consecuencias perjudiciales del estrés abiótico.

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“…Dehydrins (DHNs) are plant-specific proteins that accumulate during the abiotic stress that cause cellular dehydration, such as drought, salinity, freezing, or by treatment with the phytohormone ABA 2 . Transcriptional and proteomic studies of abiotic tolerance in several plant species have demonstrated that DHNs transcripts and protein levels increase in tolerant varieties 3 – 5 . In addition, transgenic studies have widely demonstrated the positive effect of DHNs expression and accumulation in order to survive to changing conditions 6 – 8 .…”

Section: Introductionmentioning

confidence: 99%

In vivo evidence for homo- and heterodimeric interactions of Arabidopsis thaliana dehydrins AtCOR47, AtERD10, and AtRAB18

Hernández-Sánchez

Maruri-López

Graether

et al. 2017

Sci Rep

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Dehydrins (DHNs) are intrinsically disordered proteins that play central roles in plant abiotic stress responses; however, how they work remains unclear. Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions. To explore the dimerization ability of the Arabidopsis acidic DHNs AtCOR47 and AtERD10, we conducted an in planta DHN binding assay using the Bimolecular Fluorescence Complementation (BiFC) technique. Our analyses revealed homodimeric interactions for AtCOR47 and AtERD10; interestingly, heterodimeric associations also occurred with these DHNs, and these interactions were observed in the cytosol of tobacco cells. Furthermore, we evaluated whether Arabidopsis basic DHNs, such as AtRAB18, could also interact with itself and/or with AtCOR47 and AtERD10 in the BiFC system. Our data revealed homodimeric RAB18 complexes in the nucleus and cytosol, while heterodimeric associations between AtRAB18 and acidic DHNs occurred only in the cytosol. Finally, we demonstrated the presence of heterodimeric complexes among Arabidopsis AtCOR47, AtERD10, and AtRAB18 DHNs with their acidic ortholog the OpsDHN1 from Opuntia streptacantha; these heterodimeric interactions showed different subcellular distributions. Our results guide DHN research toward a new scenario where DHN/DHN oligomerization could be explored as a part of their molecular mechanism.

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Protein Profiles and Dehydrin Accumulation in Some Soybean Varieties (<i>Glycine max</i> L. Merr) in Drought Stress Conditions

Cited by 28 publications

References 37 publications

Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton

Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton

Mecanismos de respuesta al estrés abiótico: hacia una perspectiva de las especies forestales

In vivo evidence for homo- and heterodimeric interactions of Arabidopsis thaliana dehydrins AtCOR47, AtERD10, and AtRAB18

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