Protein-protein interaction network analysis on the whiteleg shrimp Penaeus vannamei and Vibrio parahaemolyticus host-pathogen relationship reveals possible proteins and pathways involved during infection

Rosilan, Nur Fathiah; Waiho, Khor; Fazhan, Hanafiah; Sung, Yeong Yik; Nor, Siti Azizah Mohd; Muhammad, Nor Azlan Nor; Mohamed‐Hussein, Zeti‐Azura; Afiqah‐Aleng, Nor

doi:10.1016/j.aqrep.2023.101583

Cited by 4 publications

(3 citation statements)

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“…Rosilan et al [ 27 ] have conducted a host-pathogen PPI (HP-PPI) network analysis between P . vannamei and V .…”

Section: Introductionmentioning

confidence: 99%

“…vannamei were identified. Those interactions are identified between ferritin-HrpE/YscL family type III secretion apparatus protein, protein kinase domain-containing protein-chemotaxis CheY protein, and G protein-coupled receptor kinase (GPCR)-chemotaxis CheY protein [ 27 ]. This study aims to elucidate the above-mentioned interactions by determining the binding sites and evaluating their affinity using structure-based approaches such as protein-protein docking and MD simulation.…”

Section: Introductionmentioning

confidence: 99%

“…Rosilan et al [27] have conducted a host-pathogen PPI (HP-PPI) network analysis between P. vannamei and V. parahaemolyticus using interolog-and domain-based methods and several pairs of interactions that might be involved in V. parahaemolyticus infection in P. vannamei were identified. Those interactions are identified between ferritin-HrpE/YscL family type III secretion apparatus protein, protein kinase domain-containing protein-chemotaxis CheY protein, and G protein-coupled receptor kinase (GPCR)-chemotaxis CheY protein [27]. This study aims to elucidate the above-mentioned interactions by determining the binding sites and evaluating their affinity using structure-based approaches such as protein-protein docking and MD simulation.…”

Section: Introductionmentioning

confidence: 99%

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Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein interactions in Penaeus vannamei and Vibrio parahaemolyticus

Rosilan,

Jamali,

Sufira

et al. 2024

PLoS ONE

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Shrimp aquaculture contributes significantly to global economic growth, and the whiteleg shrimp, Penaeus vannamei, is a leading species in this industry. However, Vibrio parahaemolyticus infection poses a major challenge in ensuring the success of P. vannamei aquaculture. Despite its significance in this industry, the biological knowledge of its pathogenesis remains unclear. Hence, this study was conducted to identify the interaction sites and binding affinity between several immune-related proteins of P. vannamei with V. parahaemolyticus proteins associated with virulence factors. Potential interaction sites and the binding affinity between host and pathogen proteins were identified using molecular docking and dynamics (MD) simulation. The P. vannamei-V. parahaemolyticus protein-protein interaction of Complex 1 (Ferritin-HrpE/YscL family type III secretion apparatus protein), Complex 2 (Protein kinase domain-containing protein-Chemotaxis CheY protein), and Complex 3 (GPCR-Chemotaxis CheY protein) was found to interact with -4319.76, -5271.39, and -4725.57 of the docked score and the formation of intermolecular bonds at several interacting residues. The docked scores of Complex 1, Complex 2, and Complex 3 were validated using MD simulation analysis, which revealed these complexes greatly contribute to the interactions between P. vannamei and V. parahaemolyticus proteins, with binding free energies of -22.50 kJ/mol, -30.20 kJ/mol, and -26.27 kJ/mol, respectively. This finding illustrates the capability of computational approaches to search for molecular binding sites between host and pathogen, which could increase the knowledge of Vibrio spp. infection on shrimps, which then can be used to assist in the development of effective treatment.

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“…Rosilan et al [ 27 ] have conducted a host-pathogen PPI (HP-PPI) network analysis between P . vannamei and V .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein interactions in Penaeus vannamei and Vibrio parahaemolyticus

Rosilan,

Jamali,

Sufira

et al. 2024

PLoS ONE

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A visual, rapid, and sensitive detection platform for Vibrio parahaemolyticus based on RPA-CRISPR/Cas12a and an immunochromatographic test strip

Wang,

Xu,

Liu

et al. 2024

Food Quality and Safety

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Vibrio parahaemolyticus is the primary species that causes vibriosis. In this study, a point-of-care detection method was developed for V. parahaemolyticus. The detection platform targeted the thermolabile haemolysin (tlh) gene of V. parahaemolyticus based on recombinant polymerase amplification (RPA) and clustered regularly spaced short palindromic repeat (CRISPR/Cas) systems. The platform was combined with an immunochromatographic test strip (ICS) that enables low-cost, simple, and visual detection of V. parahaemolyticus. The detection limit was 2.5×102 fg/µL for plasmids and 1.4×102 CFU/mL for V. parahaemolyticus. In addition, V. parahaemolyticus in salmon sashimi could be detected at the concentration of 154 CFU/g without enrichment, and the entire detection time was ~30 min. After enrichment for 6 h, 2 CFU/g of V. parahaemolyticus could be detected. Consequently, the proposed RPA-CRISPR/Cas12a-ICS platform could detect V. parahaemolyticus in seafood intuitively, quickly, and sensitively, leading to a high practical application value.

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Growth performance, non-specific immune activity, and resistance against Vibrio parahaemolyticus of whiteleg shrimp fed dietary Chromolaena odorata leaf flour components

Harlina,

Rosmiati,

Hamdillah

et al. 2024

Fish Aquat Sci

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Protein-protein interaction network analysis on the whiteleg shrimp Penaeus vannamei and Vibrio parahaemolyticus host-pathogen relationship reveals possible proteins and pathways involved during infection

Cited by 4 publications

References 84 publications

Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein interactions in Penaeus vannamei and Vibrio parahaemolyticus

Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein interactions in Penaeus vannamei and Vibrio parahaemolyticus

A visual, rapid, and sensitive detection platform for Vibrio parahaemolyticus based on RPA-CRISPR/Cas12a and an immunochromatographic test strip

Growth performance, non-specific immune activity, and resistance against Vibrio parahaemolyticus of whiteleg shrimp fed dietary Chromolaena odorata leaf flour components

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