Protein release from alginate matrices

Gombotz, Wayne R.; Wee, Siow Fong

doi:10.1016/j.addr.2012.09.007

Cited by 1,401 publications

(233 citation statements)

References 97 publications

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“…The resultant hydrogels are non-immunogenic, porous, and thermally stable. These properties are used for drug delivery [4][5][6][7], wound treatment [4,6,8], and cell encapsulation in calcium cross-linked alginate hydrogel [8,9].…”

Section: Introductionmentioning

confidence: 99%

Photochemical Patterning of Ionically Cross-Linked Hydrogels

2013

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Iron(III) cross-linked alginate hydrogel incorporating sodium lactate undergoes photoinduced degradation, thus serving as a biocompatible positive photoresist suitable for photochemical patterning. Alternatively, surface etching of iron(III) cross-linked hydrogel contacting lactic acid solution can be used for controlling the thickness of the photochemical pattering. Due to biocompatibility, both of these approaches appear potentially useful for advanced manipulation with cell cultures including growing cells on the surface or entrapping them within the hydrogel.

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Section: Introductionmentioning

confidence: 99%

Photochemical Patterning of Ionically Cross-Linked Hydrogels

2013

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“…It has been used for cell delivery because it can be cross-linked under mild conditions by the addition of divalent cations (Gombotz and Wee, 1998). However, it is inherently non-degradable in mammals (Lee and Mooney, 2012).…”

Section: Discussionmentioning

confidence: 99%

Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering

Qiao

Dong

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To deliver cells deep into injectable calcium phosphate cement (CPC) through alginate-chitosan (AC) microcapsules and investigate the biological behavior of the cells released from microcapsules into the CPC. Methods: Mouse osteoblastic MC3T3-E1 cells were embedded in alginate and AC microcapsules using an electrostatic droplet generator. The two types of cell-encapsulating microcapsules were then mixed with a CPC paste. MC3T3-E1 cell viability was investigated using a Wst-8 kit, and osteogenic differentiation was demonstrated by an alkaline phosphatase (ALP) activity assay. Cell attachment in CPC was observed by an environment scanning electron microscopy. Results: Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC paste. The released cells attached to the setting CPC scaffolds, survived, differentiated, and formed mineralized nodules. Cells grew in the pores concomitantly created by the AC microcapsules in situ within the CPC. At Day 21, cellular ALP activity in the AC group was approximately four times that at Day 7 and exceeded that of the alginate microcapsule group (P<0.05). Pores formed by the AC microcapsules had a diameter of several hundred microns and were spherical compared with those formed by alginate microcapsules. Conclusions: AC microcapsule is a promising carrier to release seeding cells deep into an injectable CPC scaffold for bone engineering.

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“…Alginato com o seu grupo carboxílico é classificado como um polímero mucoadesivo aniônico e, em particular, as micropartículas de alginato tem apresentado essas propriedades (CRAIG; TAMBURIC, 1997;GOMEZ;WEE, 1998 PAVANETO et al, 1996, GENTA et al, 1997, RIBEIRO et al, 1999 • reduzir ou contornar a via transepidérmica;…”

Section: Micropartículas De Alginato/quitosanaunclassified

“…Existem muitos trabalhos na literatura onde esse método é empregado por diferentes grupos para obtenção de micropartículas com diâmetro menor que 200µm FANSLOW, 1995;BOWERSOCK et al, 1996;WEE, 1998 Também não foram verificadas alterações no espectro da mistura física sugerindo que não há interação fármaco/polímero nessa mistura.…”

Section: Concentraçãounclassified

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

Lira¹

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Protein release from alginate matrices

Cited by 1,401 publications

References 97 publications

Photochemical Patterning of Ionically Cross-Linked Hydrogels

Photochemical Patterning of Ionically Cross-Linked Hydrogels

Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

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