Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2-based interfaces

Blümmel, Jacques; Perschmann, Nadine; Aydin, Daniel; Drinjákovic, Jována; Surrey, Thomas; López-Garcı́a, Mónica; Kessler, Horst; Spatz, Joachim P.

doi:10.1016/j.biomaterials.2007.07.038

Cited by 195 publications

(196 citation statements)

References 33 publications

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“…and dried with N 2 gas. Polyethylene glycol (PEG) triethoxysilane (PEG2000-urea) was synthesized according to previously published protocols [23]. Prior to coating, glass cover slips were activated by piranha solution (H 2 SO 4 /H 2 O 2 = 3:1).…”

Section: Preparation Of Surfacesmentioning

confidence: 99%

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

et al. 2012

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Settlement of the planktonic dispersal stages of marine organisms is the crucial step for the development of marine biofouling. Four-dimensional holographic tracking reveals the mechanism by which algal spores select surfaces suitable for colonization. Quantitative analysis of the three dimensional swimming trajectories of motile spores of a macroalga (Ulva linza) in the vicinity of surfaces functionalized with different chemistries reveals that their search strategy and swimming behavior is correlated to the number of settled spores found in spore settlement bioassays conducted over 45 min. The spore motility and exploration behavior can be classified into different motion patterns, with their relative occurrence changing with the surface chemistry. Based on the detailed motility analysis we derived a model for the surface selection and settlement process of Ulva zoospores.

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Section: Preparation Of Surfacesmentioning

confidence: 99%

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

et al. 2012

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“…Therefore, the substrate area which was not covered by gold nanoparticles was passivated by Polyethylene Glycol (PEG) to avoid nonspecific protein adsorption. 5,25 The nanoparticles were functionalized with the c(RGDfK)-thiol cell adhesive molecules, which specifically bind to integrins. 5,9 Accordingly, each functionalized nanoparticle served as a c(-RGDfK-) adhesion patch.…”

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confidence: 99%

Induction of Cell Polarization and Migration by a Gradient of Nanoscale Variations in Adhesive Ligand Spacing

et al. 2008

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Cell interactions with adhesive surfaces play a vital role in the regulation of cell proliferation, viability and differentiation, and affect multiple biological processes. Since cell adhesion depends mainly on the nature and density of the adhesive ligand molecules, spatial molecular patterning, which enables the modulation of adhesion receptor clustering, might affect both the structural and signalling activities of the adhesive interaction. We herein show that cells plated on surfaces that present a molecularly defined spacing gradient of an integrin RGD ligand, can sense small but consistent differences in adhesive ligand spacing of about 1 nm across the cell diameter, which is approximately 61 m when the spacing includes 70 nm. Consequently, these positional cues induce cell polarization, and initiate cell migration and signalling. We propose that differential positional clustering of the integrin transmembrane receptors is used by cells for exploring and interpreting their environment, at high spatial sensitivity.Adhesion of tissue cells to the extracellular matrix depends on the activation of specific transmembrane receptors; e.g. integrins, which leads to the assembly of specialized adhesion sites known as focal adhesions (FA). 1 Activation and spatial organization of integrins are mainly controlled by epitopes containing a RGD (R = arginine, G = glycine, D = aspartate) sequence, that are present on a variety of adhesive extracellular matrix (ECM) proteins. Recent studies indicated that beyond the chemical specificity of the adhesive epitope, 2 many physical features of the adhesive surface, including its topography, 3 rigidity, 4 and precise epitope spacing, 5 are critical for guiding receptor-mediated adhesion formation and signalling. Specifically, it was demonstrated that cyclic RGDfK peptides linked to nanogold particles 6 which were arranged in pattern on substrates and interspaced by 58 or 73 nm, influence cell adhesion, 5 spreading, focal adhesion assembly, and migration 7,8 in very different ways. In these studies, control experiments demonstrated that only the specific functionalization of nanogold particles with cyclic RGD induced integrin clustering and such focal adhesion formation upon interaction with cells such as 3T3-fibroblasts or -* Corresponding author: spatz@mf.mpg.de. + Both authors contributed equally to this work. 19 On the molecular length scale, these methods enable quantification of an average molecular concentration, which gradually varies along the adhesive surface; however, they are too "noisy" to quantify the densities themselves, and density variations capable of inducing cell polarization. NIH Public AccessWe herein demonstrate a novel surface patterning technique for the generation of nanoparticle spacing gradients, which is based on controlled modulation of the selfassembly of diblock copolymer micelles. Due to the self-assembly of macromolecules, large-scale surface areas are easily processed by this technique. After successful biofunctionalization of the nano...

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“…Decreased NSA of proteins on PEGylated versus neat surfaces has been reported in several reports (see, e.g., references 35,[53][54][55][56] ). An antifouling effect of PEG was observed also in this study, where the NSA of BSA on neat PGMA-COOH, CH 3 -PEG 30,000 -NH 2 -PGMA, and CH 3 -PEG 2,000 -NH 2 -PGMA microspheres was compared (Fig.…”

Section: Nsa Of Proteinsmentioning

confidence: 60%

“…Consequently, a rapid and simple method to improve particle surface properties is required. PEG was chosen for its antifouling properties, which have been so far deeply investigated in a relation with proteins, cells, DNA [53][54][55]58 etc.…”

Section: Resultsmentioning

confidence: 99%

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Kučerová

Svobodová²,

Knotek³

et al. 2014

Materials Science and Engineering: C

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Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2-based interfaces

Cited by 195 publications

References 33 publications

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

Induction of Cell Polarization and Migration by a Gradient of Nanoscale Variations in Adhesive Ligand Spacing

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

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