Protein<scp>S</scp>

Yamazaki, Tomio; Dahlb��ck, Bj��rn

doi:10.1002/0471203076.emm0545

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…We have also demonstrated that Muse-AT cells do not form teratomas, just as was described for Muse cells obtained from bone marrow and dermal fibroblasts [41]. The pleiotropic cytokine TGF-b1 has been proposed as a stemness regulator of hematopoietic stem cells (HSCs) through phosphorylation of SMAD2, an intracellular transducer acting downstream of the TGF-b receptors [42]. HSCs require stimulation with activin-like kinase 5 (TGF-b type I receptor) ligands to maintain a quiescent state in the bone marrow niche.…”

Section: Discussionsupporting

confidence: 71%

Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-β1

Gimeno

Fuertes

Tabarrozzi

et al. 2016

Stem Cells Translational Medicine

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Adult mesenchymal stromal cell‐based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage‐differentiating stress‐enduring (Muse) cells derived from adipose tissue (AT), a subpopulation of pluripotent stem cells isolated from human lipoaspirates. Muse‐AT cells were efficiently obtained using a simple, fast, and affordable procedure, avoiding cell sorting and genetic manipulation methods. Muse‐AT cells isolated under severe cellular stress, expressed pluripotency stem cell markers and spontaneously differentiated into the three germ lineages. Muse‐AT cells grown as spheroids have a limited proliferation rate, a diameter of ∼15 µm, and ultrastructural organization similar to that of embryonic stem cells. Muse‐AT cells evidenced high stage‐specific embryonic antigen‐3 (SSEA‐3) expression (∼60% of cells) after 7–10 days growing in suspension and did not form teratomas when injected into immunodeficient mice. SSEA‐3+‐Muse‐AT cells expressed CD105, CD29, CD73, human leukocyte antigen (HLA) class I, CD44, and CD90 and low levels of HLA class II, CD45, and CD34. Using lipopolysaccharide‐stimulated macrophages and antigen‐challenged T‐cell assays, we have shown that Muse‐AT cells have anti‐inflammatory activities downregulating the secretion of proinflammatory cytokines, such as interferon‐γ and tumor necrosis factor‐α. Muse‐AT cells spontaneously gained transforming growth factor‐β1 expression that, in a phosphorylated SMAD2‐dependent manner, might prove pivotal in their observed immunoregulatory activity through decreased expression of T‐box transcription factor in T cells. Collectively, the present study has demonstrated the feasibility and efficiency of obtaining Muse‐AT cells that can potentially be harnessed as immunoregulators to treat immune‐related disorders. Stem Cells Translational Medicine 2017;6:161–173

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Section: Discussionsupporting

confidence: 71%

Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-β1

Gimeno

Fuertes

Tabarrozzi

et al. 2016

Stem Cells Translational Medicine

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“…Recently an experiment 16 has characterized coherent resonant energy transfer (CRET) in a closely related family of anthracene dimers (1). In this investigation an exciting femtosecond pulse of linearly polarized light prepares a superposition of electronic states on a pair of identical chromophores.…”

Section: Introductionmentioning

confidence: 99%

Exciton coherence lifetimes from electronic structure

Parkhill

Tempel

Aspuru‐Guzik

2012

The Journal of Chemical Physics

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We model the coherent energy transfer of an electronic excitation within covalently linked aromatic homodimers from first-principles, to answer whether the usual models of the bath calculated via detailed electronic structure calculations can reproduce the key dynamics. For these systems the timescales of coherent transport are experimentally known from time-dependent polarization anisotropy measurements, and so we can directly assess the whether current techniques might be predictive for this phenomenon. Two choices of electronic basis states are investigated, and their relative merits discussed regarding the predictions of the perturbative model. The coupling of the electronic degrees of freedom to the nuclear degrees of freedom is calculated rather than assumed, and the fluorescence anisotropy decay is directly reproduced.Surprisingly we find that although TDDFT absolute energies are routinely in error by orders of magnitude more than the coupling energy, the coherent transport properties of these dimers can be semi-quantitatively reproduced from first-principles.The directions which must be pursued to yield predictive and reliable prediction of coherent transport are suggested.

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ProteinS

Cited by 2 publications

References 11 publications

Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-β1

Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-β1

Exciton coherence lifetimes from electronic structure

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