Protein Synthesis and Oxygen Consumption of the Shore Crab Carcinus maenas after a Meal

Houlihan, D.F.; Waring, Colin P.; Mathers, E.; Gray, Colin

doi:10.1086/physzool.63.4.30158174

Cited by 110 publications

(67 citation statements)

References 40 publications

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“…The ranking of the tissues in terms of RNA to protein ratios was as previously described (21). There was no significant change in the concentration of RNA with hypoxia in the tissues either expressed in relation to wet weight or protein.…”

Section: Resultsmentioning

confidence: 75%

“…Rates of protein synthesis in tissues were determined after the injection of a single "flooding dose" of [ 3 H]phenylalanine (21). The injection solution consisted of 135 mM phenylalanine and L-[2,6-3 H]phenylalanine (Amersham) at 100 Ci/ml (3.7 MBq/ml) at a dose of 1.0 ml ⅐ 100 g Ϫ1 ⅐ live wt Ϫ1 .…”

Section: Methodsmentioning

confidence: 99%

“…The injection was made into the blood sinus at the base of the third walking leg. No O 2 consumption measurements were made on these animals because of the elevation of the O2 consumption that occurs through handling (21).…”

Section: Methodsmentioning

confidence: 99%

“…Protein determinations were treated as described in Ref. 21. The RNA content and the RNA activity (k RNA, the amount of protein in g being synthesized per g of RNA per day) were calculated as described in Ref.…”

Section: Methodsmentioning

confidence: 99%

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Influence of oxygen partial pressures on protein synthesis in feeding crabs

Mente

Legeay

Houlihan

et al. 2003

American Journal of Physiology-Regulatory, Integrative and Comp

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. Influence of oxygen partial pressures on protein synthesis in feeding crabs. Am J Physiol Regul Integr Comp Physiol 284: R500-R510, 2003; 10.1152/ ajpregu.00193.2002.-Many water-breathing animals have a strategy that consists of maintaining low blood PO 2 values in a large range of water oxygenation level (4-40 kPa). This study examines the postprandial changes in O 2 consumption, arterial blood PO2, and tissue protein synthesis in the shore crab Carcinus maenas in normoxic, O2-depleted, and O2-enriched waters to study the effects of this strategy on the O2 consumption and peptide bond formation after feeding. In normoxic water (21 kPa), the arterial PO2 was 1.1 kPa before feeding and 1.2 kPa 24 h later. In water with a PO2 of 3 kPa (arterial PO2 0.6 kPa), postprandial stimulation of protein synthesis and O2 consumption were blocked. The blockade was partial at a water PO2 of 4 kPa (arterial PO2 0.8 kPa). An increase in environmental PO2 (60 kPa, arterial PO2 10 kPa) resulted in an increase in protein synthesis compared with normoxic rates. It is concluded that the arterial PO2 spontaneously set in normoxic Carcinus limits the rates of protein synthesis. The rationale for such a strategy is discussed. crustaceans; oxygen consumption; blood oxygen; hypoxia IN AIR-BREATHING HOMEOTHERMS the PO 2 in the arterial blood is regulated at 10-14 kPa in resting conditions at sea level (Ref. 6; for reference, PO 2 in air at sea level is 20-21 kPa). At the cellular level the most frequently measured values are in the 1-to 3-kPa range in the tissues (43). In numerous water breathers and other poikilotherms, the arterial PO 2 is also set at 1-3 kPa (reviewed in Ref. 27). It has been suggested that these low PO 2 values are part of a strategy of cell oxygenation termed "the low tissue O 2 strategy," which evolved with the origin of aerobic metabolism (27).In water-breathing animals, there is a strategy of gas-exchange regulation that consists of maintaining PO 2 in the arterial blood in an astonishingly low and narrow range, about 5-10 times lower than in homeotherms. Previous studies in crustaceans reported that O 2 , working in the low PO 2 range, acts 1) in a neuromodulator-like manner on the neural networks that generate the masticating and filtering movements of the foregut (5, 31) and 2) limits the oxidative metabolism of locomotor muscles (12).This paper aims to determine whether the low tissue O 2 strategy could affect protein metabolism in a water breather. Around 60 O 2 -dependent reactions exhibit values of the Michaelis-Menten constant for O 2 (K m,O 2 ) that would be rate limited at likely tissue O 2 levels (except for cytochrome-c oxidase; Ref. 43). Besides these reactions there may also be a limitation on the rate of energy supply for other processes. If these conditions are present in vivo in water breathers, we would expect that 1) an increase in tissue energy demand would result in an increase in blood PO 2 to drive an increase in tissue oxygenation and/or an increase in blood flow; 2) a reduction in envir...

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Section: Resultsmentioning

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Influence of oxygen partial pressures on protein synthesis in feeding crabs

Mente

Legeay

Houlihan

et al. 2003

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Because protein synthesis represents the major energetic cost of processing a meal, post-prandial changes in protein synthesis have been studied in a range of animal taxa: crustaceans (e.g. Houlihan et al, 1990;Whiteley et al, 2001), fishes (e.g. McMillan and Houlihan, 1988;Lyndon et al, 1992;Katersky and Carter, 2010), birds (e.g.…”

Section: Introductionmentioning

confidence: 99%

Post-prandial changes in protein synthesis in red drum (Sciaenops ocellatus) larvae

McCarthy

Fuiman

2011

Journal of Experimental Biology

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SUMMARYProtein synthesis is one of the major energy-consuming processes in all living organisms. Post-prandial changes in protein synthesis have been studied in a range of animal taxa but have been little studied in fish larvae. Using the flooding-dose method, we measured post-prandial changes in whole-body rates of protein synthesis in regularly fed red drum Sciaenops ocellatus (Linnaeus) larvae for 24-28h following their daily meal. Fractional rates of protein synthesis increased from a baseline (prefeeding) rate of 16%day -1 to a post-prandial peak of 48%day -1 ca. 8h after feeding before declining to 12%day -1 after 24-28h. The overall mean daily rate of protein synthesis was calculated as 27%day -1 . Although suggested as energetically impossible in larval poikilotherms, our results show that rates in excess of 30%day -1 can be attained by larval fishes for a few hours but are not sustained. The average daily energetic cost of protein synthesis was estimated as 34% of daily total oxygen consumption, ranging from 19% immediately before feeding to 61% during the post-prandial peak in protein synthesis. This suggests that during the post-prandial peak, protein synthesis will require a large proportion of the hourly energy production, which, given the limited metabolic scope in fish larvae, may limit the energy that could otherwise be allocated to other energy-costly functions, such as foraging and escape responses.

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Past energy allocation overwhelms current energy stresses in determining energy allocation trade‐offs

Griffen

Bolander

Blakeslee

et al. 2023

Ecology and Evolution

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Regeneration of lost appendages is a gradual process in many species, spreading energetic costs of regeneration through time. Energy allocated to the regeneration of lost appendages cannot be used for other purposes and, therefore, commonly elicits energetic trade‐offs in biological processes. We used limb loss in the Asian shore crab Hemigrapsus sanguineus to compare the strength of energetic trade‐offs resulting from historic limb losses that have been partially regenerated versus current injuries that have not yet been repaired. Consistent with previous studies, we show that limb loss and regeneration results in trade‐offs that reduce reproduction, energy storage, and growth. As may be expected, we show that trade‐offs in these metrics from historic limb losses far outweigh trade‐offs from current limb losses, and correlate directly with the degree of historic limb loss that has been regenerated. As regenerating limbs get closer to their normal size, these historical injuries get harder to detect, despite the continued allocation of additional resources to limb development. Our results demonstrate the importance of and a method for identifying historic appendage losses and of quantifying the amount of regeneration that has already occurred, as opposed to assessing only current injury, to accurately assess the strength of energetic trade‐offs in animals recovering from nonlethal injury.

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Protein Synthesis and Oxygen Consumption of the Shore Crab Carcinus maenas after a Meal

Cited by 110 publications

References 40 publications

Influence of oxygen partial pressures on protein synthesis in feeding crabs

Influence of oxygen partial pressures on protein synthesis in feeding crabs

Post-prandial changes in protein synthesis in red drum (Sciaenops ocellatus) larvae

Past energy allocation overwhelms current energy stresses in determining energy allocation trade‐offs

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