Proteinase K: Eine neue, serologisch anwendbare Pilz-Protease

Roelcke, D.; Uhlenbruck, G.

doi:10.1007/bf02123859

Cited by 8 publications

(6 citation statements)

References 14 publications

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“…A review dealing with pronase as a serologically suitable enzyme was given by UHLENBRUCK [38]. Recently subtilisin and proteinase K have been recommended for blood group testing [40,32]. That it is possible to distinguish between anti-I/i and anti-PrJPr, by red cell treatment with proteinase K has been described by ROELCKE and UHLENBRUCK [32].…”

Section: Immunoelectrophoresis and Double D I F I I O N Testmentioning

confidence: 99%

“…Recently subtilisin and proteinase K have been recommended for blood group testing [40,32]. That it is possible to distinguish between anti-I/i and anti-PrJPr, by red cell treatment with proteinase K has been described by ROELCKE and UHLENBRUCK [32]. Neuraminidase (RDE) from Vibrio cholerae was obtained from Behringwerke, Marburg.…”

Section: Immunoelectrophoresis and Double D I F I I O N Testmentioning

confidence: 99%

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IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

et al. 1971

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Abstract. Two IgG‐type cold auto‐agglutinins causing transiently occurring haemolytic anaemia in children are described. The antibodies were limited to the IgG fraction obtained by DEAE cellulose chromatography and were resistant to 2‐mercaptoethanol treatment. One auto‐agglutinin reacted ‘incompletely’ (in the enzyme test), the other reacted ‘completely’ and its reactivity was furthermore abolished by treatment of red cells with proteolytic enzymes, but not with neuraminidase. Because of the inactivation of the corresponding antigen on red cells by proteolytic enzymes, this receptor belongs to the Pr complex, but it can be distinguished from Pr1/Pr2 through its resistance to neuraminidase. Therefore a new symbol ‘Pra’ is proposed. Based on these findings an immunochemical classification for antigens corresponding to cold auto‐antibodies is given and a new nomenclature of the antigens which are inactivated by proteases is proposed depending on their determination or non‐determination by neuraminic acid.

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Section: Immunoelectrophoresis and Double D I F I I O N Testmentioning

confidence: 99%

Section: Immunoelectrophoresis and Double D I F I I O N Testmentioning

confidence: 99%

IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

et al. 1971

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“…Our first assumption was that it could be a compound of a peptide nature. To probe this hypothesis, we tested the effect of proteinase K (PTK), an enzyme that is widely used to degrade protein components [31][32][33]. As shown in Figure 3A,C, CM treatment with PTK did not significantly reduce the value of 𝑋 compared to that obtained by dye transfer assays when Another approach that we assayed, to test this hypothesis, was to compare the time course of the capability of CM to induce GJIC in MDCK-I with that of GJIC by ouabain in MDCK-S. For the former condition, we varied the time (in a range of 0-120 min) that MDCK-S cells were treated with ouabain before extracting the CM, then treating MDCK-I during a fixed time of 30 min before making dye transfer trials.…”

Section: The Molecular Component Contained In CM That Causes Gjic In ...mentioning

confidence: 99%

Ouabain Enhances Gap Junctional Intercellular Communication by Inducing Paracrine Secretion of Prostaglandin E2

Toro

Jimenez

Rubi

et al. 2021

IJMS

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Ouabain is a cardiac glycoside that has been described as a hormone, with interesting effects on epithelial physiology. We have shown previously that ouabain induces gap junctional intercellular communication (GJIC) in wild, sensitive cells (MDCK-S), but not in cells that have become insensitive (MDCK-I) by modifying their Na+-K+-ATPase. We have also demonstrated that prostaglandin E2 (PGE2) is able to induce increased GJIC by a mechanism other than ouabain, that does not depend on Na+-K+-ATPase. In this work we show, by dye transfer assays, that when MDCK-S and MDCK-I are randomly mixed, to form monolayers, the latter stablish GJIC, because of stimulation by a compound released to the extracellular media, by MDCK-S cells, after treatment with ouabain, as evidenced by the fact that monolayers of only MDCK-I cells, treated with a conditioned medium (CM) that is obtained after incubation of MDCK-S monolayers with ouabain, significantly increase their GJIC. The further finding that either (1) pre-treatment with COX-2 inhibitors or (2) addition to CM of antagonists of EP2 receptor abolish CM’s ability to induce GJIC in MDCK-I monolayers indicate that PGE2 is the GJIC-inducing compound. Therefore, these results indicate that, in addition to direct stimulation, mediated by Na+-K+-ATPase, ouabain enhances GJIC indirectly through the paracrine production of PGE2.

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“…Recently subtilisin and proteinase K have been recommended for blood group testing [40,32]. That it is possible to distinguish between anti-I/i and anti-Pr1/Pr2 by red cell treatment with proteinase K has been described by Roelcke and Uhlenbruck [32], Neuraminidase (RDE) from Vibrio cholerae was obtained from Behringwerke, Marburg. 500 fig/ml were diluted with 9 ml saline, 1 ml packed red cell sediment was incubated with this solution for 30 min at 37°C and the thrice washed erythrocytes were used as a 2-per cent suspension in saline.…”

mentioning

confidence: 99%

“…A review dealing with pronase as a serologically suitable enzyme was given by Uhlenbruck [38]. Recently subtilisin and proteinase K have been recommended for blood group testing [40,32]. That it is possible to distinguish between anti-I/i and anti-Pr1/Pr2 by red cell treatment with proteinase K has been described by Roelcke and Uhlenbruck [32], Neuraminidase (RDE) from Vibrio cholerae was obtained from Behringwerke, Marburg.…”

mentioning

confidence: 99%

IgG-Type Cold Agglutinins in Children and Corresponding Antigens

Anstee¹,

Jungfer²,

Nutzenadel³

et al. 1971

Vox Sanguinis

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Two IgG-type cold auto-agglutinins causing transiently occurring haemolytic anaemia in children are described. The antibodies were limited to the IgG fraction obtained by DEAE cellulose chromatography and were resistant to 2-mercaptoethanol treatment. One auto-agglutinin reacted ‘incompletely’ (in the en-zyme test), the other reacted ‘completely’ and its reactivity was furthermore abolished by treatment of red cells with proteolytic enzymes, but not with neuraminidase. Because of the inactivation of the corresponding antigen on red cells by proteolytic enzymes, this receptor belongs to the Pr complex, but it can be distinguished from Pr(1)/Pr(2), through its resistance to neuraminidase. Therefore a new symbol ‘Pr(a)’ is proposed. Based on these findings an immunochemical classification for antigens corresponding to cold auto-antibodies is given and a new nomenclature of the antigens which are inactivated by proteases is proposed depending on their determination or nondetermination by neuraminic acid.

show abstract

Proteinase K: Eine neue, serologisch anwendbare Pilz-Protease

Cited by 8 publications

References 14 publications

IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

Ouabain Enhances Gap Junctional Intercellular Communication by Inducing Paracrine Secretion of Prostaglandin E2

IgG-Type Cold Agglutinins in Children and Corresponding Antigens

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