Proteins encoded by bovine viral diarrhea virus: The genomic organization of a pestivirus

Collett, Marc S.; Larson, Ruby J.; Belzer, Susan; Retzel, Ernest F.

doi:10.1016/0042-6822(88)90673-3

Cited by 264 publications

(210 citation statements)

References 13 publications

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“…Co-and posttranslational processing gives rise to the individual viral proteins. At the N terminus of the polyprotein, an autoprotease (N pr°) is followed by the structural proteins namely the viral core protein (C) and three glycoproteins (E0-E1-E2) (Collett et al, 1988b;Stark et al, 1990Stark et al, , 1993. Non-structural proteins are located in the Cterminal two-thirds of the pestiviral polyprotein.…”

Section: Introductionmentioning

confidence: 99%

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Pauly¹,

Elbers²,

König³

et al. 1995

Journal of General Virology

114

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Section: Introductionmentioning

confidence: 99%

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Pauly¹,

Elbers²,

König³

et al. 1995

Journal of General Virology

114

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“…BVDV is an enveloped virus with an uncapped and non-polyadenylated positive-stranded RNA genome of about 12.5 kb in size. The genome comprises a single open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) [11,12,14]. The 5'UTR function is a so-called internal ribosome entry site (IRES) that promotes cap-independent translation initiation [30,31].…”

Section: Introductionmentioning

confidence: 99%

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Bálint¹,

Baule

Pálfi³

et al. 2005

Vet. Res.

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-A live bovine viral diarrhea (BVDV) vaccine, marketed as a derivate of the Oregon C24V strain, was used between the end of the 1960s and the beginning of the 1990s in Central Europe. Since laboratory investigations of mucosal disease cases in vaccinated animals suggested recombinations between the vaccine and wild type variants of BVDV, and recombinational nucleotide sequences seemed distinct from BVDV Oregon C24V, the aim of the present retrospective study was to analyze the genomes of pre-registration (termed here BVDV-Xpre) and of marketed (BVDV-X) batches of the vaccine. The results of the complete genome analysis of BVDV-Xpre confirmed that the original virus strain used at the start of the vaccine production was Oregon C24V. Surprisingly, the analysis of the complete nucleotide sequence of the BVDV-X marketed vaccine revealed that this strain belongs to the BVDV 1b subgroup, with a 93.7% nucleotide sequence homology to BVDV reference strain Osloss. The homology to BVDV Oregon C24V was significantly lower (77.4%), and a thorough sequence scanning showed that the genome of BVDV-X had not derived from Oregon C24V. These data indicate the very likely scenario that a strain different to Oregon C24V was picked up during the in vitro or in vivo passages for vaccine development. Despite of the virus-switch, the BVDV-X vaccine continuously maintained its innocuity and efficacy, as proven by the regular quality testing data, and the presence of the foreign virus remained unnoticed over many years. The results of this work emphasize that the contamination of commercially available live vaccines with exogenous BVDV strains is a real risk factor, and a unequivocal analysis, including molecular methods, is needed to verify their authenticity. bovine viral diarrhea virus / vaccine / strain switch / control

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“…In addition, the comparison of pestiviruses indicated that processing of E2 involved typical hydrophobic cleavage sites for signal peptidase [21]. This information indicated that the N-terminus of E2 started from amino acid 693 (the numbers indicate amino acid positions described by Collett et al [5]). Although the C-terminal region of E2 is not still clear, it had been suggested to be around amino acid 1130.…”

mentioning

confidence: 99%

“…Besides clinically observed diarrhea, it frequently causes fever, oral ulceration, cough, leukemia and persistent infection in some cases [1,15]. BVDV like other pestiviruses consists of a single stranded RNA of positive polarity, which encodes all the structural proteins and replication components [4,5,16,17,24]. Recently, pestiviruses were classified the genus of Flaviviridae mainly based on their genetic organization and translational properties [7].…”

mentioning

confidence: 99%

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Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells.

Kweon

Yoon

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The gene encoding the envelope glycoprotein (E2) of bovine viral diarrhea virus (BVDV) was expressed in a baculovirus. The expressed protein was detected on the surface of infected cells by immunofluorescence. Western blotting analysis showed the presence of the expressed protein of a similar molecular size to the E2 protein. The antigenicity of expressed protein were tested in guinea pigs and cattle. The immunized animals developed neutralizing antibodies against BVDV. -KEY WORDS: BVDV, envelope protein (E2), expression.

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Proteins encoded by bovine viral diarrhea virus: The genomic organization of a pestivirus

Cited by 264 publications

References 13 publications

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells.

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