Proteins in Fluids From Different Segments of the Rat Epididymis

Koskimies, Aarne I.; Kormano, Martti

doi:10.1530/jrf.0.0430345

Cited by 79 publications

(25 citation statements)

References 8 publications

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“…There is a high concentration of protein in epididymal plasma from rats (Back et al, 1974) and yet a barrier has been shown to exist towards entry of large molecules from blood. The proteins appearing in the perfusate, therefore, are those specific to the epi¬ didymis (Koskimies & Kormano, 1975;Cameo & Blaquier, 1976). Tritiated water in plasma equilibrated with the perfusate in the epididymal lumen within 30 min; in hamsters the equilibration time after micropuncture was 50 min .…”

Section: Discussionmentioning

confidence: 99%

Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat

Cooper¹,

Waites²

1979

Reproduction

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Summary. Controlled perfusion through the lumen of the distal cauda epididymidis in the anaesthetized rat has been explored as a means of examining physiological exchanges from blood across the epididymal epithelium. The mean length of the perfused, sperm-free, tubule was 14\m=.\5cm (\m=+-\1\m=.\5s.e.m., n = 9). No cholesterol, protein or sialic acid was detected in the perfusate at flow rates exceeding 10 \g=m\l /mi n, but at rates of 0\ m=. \ 4\ p=n-\ 1\ m=. \ 2 \g=m\l/min,protein appeared at concentrations of 0\m=.\21\p=n-\0\m=.\55 mg/ml (i.e. secretion rates of 0\m=.\21\p=n-\0\m=.\83 \g=m\g/min; 3 rats). Glucose was detected at all perfusion rates (3\p=n-\27\g=m\l/min)at concentrations of 0\m=.\06\p=n-\0\m=.\58 mM (0\ m=. \ 8\ p=n-\ 6\ m=. \ 8% blood levels). This evidence suggests that the preparation is physiological and could be used to explore the dynamics of exchanges between blood and epididymis.

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Section: Discussionmentioning

confidence: 99%

Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat

Cooper¹,

Waites²

1979

Reproduction

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“…Consequently, the acquisition of a mature motility pattern may not be sufficient for fertilization. b) A higher embryonic mortality after insemination with young epididymal spermatozoa has been reported in the rabbit (Orgebin-Crist, 1969) and the pig (Hunter et al, 1976), but was not observed by Overstreet and Bedford (1976) (Gledhill, 1971 ;Marushige and Marushige, 1975), disulfide bonds (Bedford, 1974), ADN-Feulgen (Esnault et al, 1975) (Fournier, 1968 ;Fournier-Delpech et al, 1973, 1974Koskimies and Kormano, 1975 ;Oscaretal., 1978) and particular metabolism (Voglmayr, 1975 ;Dacheux et al, 1978 …”

Section: Methodsmentioning

confidence: 96%

Epididymal sperm maturation in the ram : motility, fertilizing ability and embryonic survival after uterine artificial insemination in the ewe

Fournier-Delpech¹,

Colas²,

Courot³

et al. 1979

Ann. Biol. anim. Bioch. Biophys.

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Summary. Ram spermatozoa from the distal caput, corpus, and cauda epididymis and ejaculated spermatozoa were artificially inseminated in 170 ewes which had been treated with an ovulatory dose of PMSG 61 ! 4 hrs previously. Results were expressed as the percent of ewes pregnant 18 days after insemination (F = with positive progesterone assay/ Q inseminated) and embryonic survival (ES = ewes lambing/ pregnant on day 18). F =0, 8, 12, 54, 78, 80 and 72 p. 100 respectively after insemination with 20 x 10 6 total spermatozoa/horn from the caput, corpus, I, II and III, proximal cauda, distal cauda, and ejaculate.Development of sperm fertilizing ability parallelled the development of sperm motility. However when ewes were inseminated with high number of motile spermatozoa, ES was 0, 77, 86, 100 and 86 p. 100 with spermatozoa from the corpus I --!-II, the corpus III, the proximal cauda, the distal cauda, and the ejaculate respectively. Thus, the sperm from middle corpus are totally unable to produce viable lambs.In the ram the development of sperm fertilizing ability in the epididymis coincides with the development of progressive motility (Fournier-Delpech et al., 1977) in a maturation process similar to the one described in rodents (reviews of OrgebinCrist et al.,1975OrgebinCrist et al., , 1976Bedford, 1975) or in the pig (Holtz and Smidt, 1976 ;Hunter et al., 1976). However, in the rabbit embryonic mortality is increased after fertilization by immature spermatozoa from the corpus epididymis (Orgebin-Crist, 1969 ; OrgebinCrist and Jahad, 1977). Similarly, litter size in rats is smaller after intra-uterine insemination with epididymal spermatozoa from the corpus than after insemination with spermatozoa from the cauda (Paz et al., 1978 (Colas, 1975 and1979 ;Gordon, 1975 a) After insemination with a constant number of spermatozoa (20 x 10 6 per horn) the pregnancy rate developed constantly which is correlated with the number of progressive motile cells inseminated per horn in the corpus (r = 0.9 in the middle corpus ; r = 0.6 in the distal corpus). However, the plateau is reached at a lower motile sperm concentration and the pregnancy rate is higher when more mature spermatozoa are inseminated ( fig. 2).Progressive motility may be the only essential factor to deliver the fertilizing agent to the oocyte for fertilization. However, development of motility is not definite proof of fertilizing ability for a potential fertile sperm. The pregnancy rate showed that fertilizing ability had not been completely achieved in the middle corpus (60 p. 100 with 20 X 10 6 motile spermatozoa inseminated/horn from the distal corpus versus 90 p. 100 with 8 x 10 6 motile spermatozoa from the distal corpus). Consequently, the acquisition of a mature motility pattern may not be sufficient for fertilization. b) A higher embryonic mortality after insemination with young epididymal spermatozoa has been reported in the rabbit (Orgebin-Crist, 1969) and the pig (Hunter et al., 1976), but was not observed by Overstreet and Bedford (1976) (Fournier...

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“…Proteins are reabsorbed (Koskimies & Kormano, 1975;Djakiew & Jones, 1983) and secreted by the extratesticular ducts of mammals. The occurrence of proteins which are not present in blood or rete testis fluid have been demonstrated in luminal fluids from the epididymis of all mammals which have been studied.…”

Section: Introductionmentioning

confidence: 99%

“…Jones et al (1984) described the structural differentiation of the epididymis of the tammar into 3 main segments, an initial, middle and terminal segment, and showed that this segmentation corresponds to the anatomical differen¬ tiation into the caput, corpus and cauda epididymidis. Jones et al (1984) concluded that the tammar may be a suitable animal for micropuncture studies of the epididymis since it would be possible to collect micropuncture samples from the initial segment free of the contamination with stereocilia which is characteristic of samples from the other mammals which have been studied (Levine & Marsh, 1971; Koskimies & Kormano, 1975;Hinton et al, 1980;Jenkins et al, 1980;Djakiew & Jones, 1983;Turner, 1984 (Djakiew & Jones, 1983 haematocrit and blood pressure were initially 450 + 2-9% and 123-6 + 44mmHg respectively, and remained fairly constant during the experimental period. The values were much the same in the remaining animals.…”

Section: Introductionmentioning

confidence: 99%

Changes in protein composition of the luminal fluids along the epididymis of the tammar, Macropus eugenii

Jones¹

1987

Reproduction

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Proteins in Fluids From Different Segments of the Rat Epididymis

Cited by 79 publications

References 8 publications

Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat

Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat

Epididymal sperm maturation in the ram : motility, fertilizing ability and embryonic survival after uterine artificial insemination in the ewe

Changes in protein composition of the luminal fluids along the epididymis of the tammar, Macropus eugenii

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