Proteins in Relation to Vigor and Viability of White Lupin (Lupinus albus L.) Seed Stored for 26 Years

Abstract

The aim of the study was to evaluate the vigor and viability as well as to determine and compare the contents of selected protein fractions of white lupin (Lupinus albus L.) seeds stored for 26 years at temperatures of -14°C and +20°C. The seeds stored at -14°C germinated in 86.3%, while the seeds stored at +20°C did not germinate at all. The viability evaluation was confirmed by the measuring electroconductivity of seed exudates. In seeds stored at -14°C the contents of γ, δ, and β conglutin were 14, 4 and 69 mg g-1 fresh mass, respectively, while in seed stored at +20°C they were 15.5, 3, 65 mg g-1 fresh mass, respectively. One-dimensional electrophoresis of γ and δ conglutin fractions indicated the presence of several intense polypeptide bands with molecular weights from 23.0 to 10.3 kDa. Polypeptide bands with a molecular weight of 22.4 and 19.8 kDa exhibited almost two times higher expression in the seeds stored at -14°C compared to the seeds stored at +20°C. Electrophoresis revealed 310 protein spots on the maps generated for seeds stored at -14°C, and 228 spots for seeds stored at +20°C. In seeds stored at +20°C most polypeptide subunits had a pI ranging from 4.5 to 7 and a molecular weight of 10–97 kDa. The greatest differences in the contents of polypeptides between the analyzed variants was observed within the range of 20–45 kDa (-14°C: 175, +20°C: 115 protein spots) and within the range of 65–97 kDa (-14°C: 103, +20°C: 75 protein spots). In seeds stored at +20°C, a clear decline in basic (8–10 pI) polypeptides was observed. The study demonstrated that the polypeptides identified as γ and δ conglutins are probably closely related to vigor and viability of seeds.

Comparative analysis of seed size, germination, and vegetative allocation in annual and herbaceous perennial crops and their wild relatives inLupinusandPhaseolus(Fabaceae)

“…Data presented here demonstrated higher germination proportion in wild annual Lupinus relative to wild perennial congeners. Similarly high germination has been observed in other studies of annual Lupinus species (Schaal, 1980; including in L. albus after 26 years in frozen storage, Dobiesz & Piotrowicz-Cieslak, 2017), and similarly low germination proportion has been observed in other perennial western North American Lupinus species (Greipsson & El-Mayas, 2003; Sõber & Ramula, 2013). Pitelka (1977) found that while North American herbaceous perennials had somewhat higher germination than annual Lupinus in field sites, both groups had less than 40% germination.…”

High-quality genome sequence of white lupin provides insight into soil exploration and seed quality

“…6c, Supplementary Data 14), which are precursor forms normally synthesized in developing cotyledons to give rise to mature polypeptides of lower molecular weight. Their degradation starts as part of the germination process and the appearance of simpler forms in the domesticated variety AMIGA could be linked with its increased seed vigor 44 . The long chain ß-conglutins present in the wild accession is also associated with a high allergenicity of lupin seeds 45 Measurement of the abundance of key alkaloids in various organs revealed that the modern accession AMIGA has very low levels of lupanine, 13-hydroxylupanine and 13-tigloyloxylupanine ( Fig.…”

A Strong Impact of Soil Tetracycline on Physiology and Biochemistry of Pea Seedlings

“…The above-ground parts of pea seedlings were ground to a powder in liquid nitrogen. Protein isolation and separation by the 2D electrophoresis method was carried out according to Dobiesz and Piotrowicz-Cieślak [30]. The peptide separation and determination of peptide masses was carried out using the Orbitrap spectrometer (Thermo).…”