Proteogenomic insights into uranium tolerance of a Chernobyl's Microbacterium bacterial isolate

Gallois, Nicolas; Alpha-Bazin, Béatrice; Ortet, Philippe; Barakat, Mohamed; Piette, Laurie; Long, Justine; Berthomieu, Catherine; Armengaud, Jean; Chapon, Virginie

doi:10.1016/j.jprot.2017.11.021

Cited by 36 publications

(34 citation statements)

References 63 publications

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“…M. oleivorans A9 cells were isolated from Chernobyl trench T22 soil [5] and exposed as described [1] . Briefly, cells were harvested and the cell pellets were resuspended in 0.1 M NaCl pH 5.0 with 0 (control) or 10 µM uranyl nitrate.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…After centrifugation, the resulting supernatants were removed and the pellets were conserved at -80 °C until proteomic analysis. Proteogenomics were carried out as previously described [1] , [6] taking into account the recent draft genome of the strain.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The recorded MS/MS spectra for the 24 samples were searched against our home-made ORF database with the parameters described previously [1] . This database contains 30,853 polypeptide sequences, for a total of 4,903,573 amino acids with an average of 159 amino acids per polypeptide.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“… The data have been used to define the proteome changes in response to uranium stress in an Actinobacteria isolate from the trench T22 located near the Chernobyl nuclear power plant. As described in detail in the accompanying manuscript [1] , the uranyl stress perturbed the phosphate and iron metabolic pathways. …”

mentioning

confidence: 89%

“…The proteome data from four independent biological replicates per time point (0.5, 4 and 24 h) upon uranium exposure or control, i.e. from 24 samples, were assigned to tryptic peptides against the M. oleivorans A9 ORF database described by Gallois et al [1] following a proteogenomic approach [2] , [3] , [4] . The deposited data comprised the 24 raw files and the interpreted files.…”

Section: Datamentioning

confidence: 99%

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Proteomics data for characterizing Microbacterium oleivorans A9, an uranium-tolerant actinobacterium isolated near the Chernobyl nuclear power plant

et al. 2018

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Microbacterium oleivorans A9 cells were exposed or not to 10 µM uranyl nitrate as resting cells in sodium chloride solution. Bacteria exposed to U(VI) and controls were harvested after 0.5, 4, and 24 h of toxicant exposure. Bacteria were subjected to high-throughput proteomics analysis using a Q-Exactive HF high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. MS/MS spectra were assigned with a protein sequence database derived from a draft genome obtained by Illumina sequencing and systematic six-reading frame translation of all the contigs. Proteins identified in bacteria exposed to U(VI) and controls at the three time points allow defining the proteome dynamics upon uranium stress. The data reported here are related to a published study regarding the proteome dynamics of M. oleivorans A9 upon uranium stress by Gallois et al. (in press) entitled “Proteogenomic insights into uranium tolerance of a Chernobyl׳s Microbacterium bacterial isolate”. The data accompanying the manuscript describing the database searches and comparative analysis have been deposited to the ProteomeXchange with identifier PXD005794.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

mentioning

confidence: 89%

Section: Datamentioning

confidence: 99%

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Proteomics data for characterizing Microbacterium oleivorans A9, an uranium-tolerant actinobacterium isolated near the Chernobyl nuclear power plant

et al. 2018

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Environmental Radiobiology

Lourenço,

Mothersill,

Arena

et al. 2023

Radiobiology Textbook

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This chapter aims to contribute to a comprehensive view of environmental radiobiology and discuss the effects of different kinds of ionizing radiation on ecosystems. The impact of ionizing radiation was considered on both organisms and the abiotic environment, assessing the fate of radionuclides in abiotic compartments (e.g., the movement through atmosphere, hydrosphere, and lithosphere) and in the trophic chains, with implications for human and non-human biota. The available methodologies for estimating radiation dose to biota were also addressed as well as the associated challenges. This chapter also focused on the impacts of ionizing radiation exposure on non-human biota from microorganisms to vertebrates, as well as on the basic concepts related to environmental radiobiology and the molecular effects associated with the exposure to different types of ionizing radiation. The particular context of Naturally Occurring Radioactive Material (NORM) contamination was also tackled, as well as its effects on non-human biota.

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Biostimulation of indigenous microbes for uranium bioremediation in former U mine water: multidisciplinary approach assessment

Newman-Portela,

Krawczyk-Bärsch,

Lopez-Fernandez

et al. 2023

Environ Sci Pollut Res

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Characterizing uranium (U) mine water is necessary to understand and design an effective bioremediation strategy. In this study, water samples from two former U-mines in East Germany were analysed. The U and sulphate (SO42−) concentrations of Schlema-Alberoda mine water (U: 1 mg/L; SO42−: 335 mg/L) were 2 and 3 order of magnitude higher than those of the Pöhla sample (U: 0.01 mg/L; SO42−: 0.5 mg/L). U and SO42− seemed to influence the microbial diversity of the two water samples. Microbial diversity analysis identified U(VI)-reducing bacteria (e.g. Desulfurivibrio) and wood-degrading fungi (e.g. Cadophora) providing as electron donors for the growth of U-reducers. U-bioreduction experiments were performed to screen electron donors (glycerol, vanillic acid, and gluconic acid) for Schlema-Alberoda U-mine water bioremediation purpose. Thermodynamic speciation calculations show that under experimental conditions, U(VI) is not coordinated to the amended electron donors. Glycerol was the best-studied electron donor as it effectively removed 99% of soluble U, 95% of Fe, and 58% of SO42− from the mine water, probably by biostimulation of indigenous microbes. Vanillic acid removed 90% of U, and no U removal occurred using gluconic acid.

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Proteogenomic insights into uranium tolerance of a Chernobyl's Microbacterium bacterial isolate

Cited by 36 publications

References 63 publications

Proteomics data for characterizing Microbacterium oleivorans A9, an uranium-tolerant actinobacterium isolated near the Chernobyl nuclear power plant

Proteomics data for characterizing Microbacterium oleivorans A9, an uranium-tolerant actinobacterium isolated near the Chernobyl nuclear power plant

Environmental Radiobiology

Biostimulation of indigenous microbes for uranium bioremediation in former U mine water: multidisciplinary approach assessment

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