Proteoglycans from sheep, pig, rat and human spleens having chemical and biological resemblances to that in Kurloff cells

Dean, Michael F.; Muir, Helen; Marshall, A. H. E.; Revell, Peter A.; Vernon‐Roberts, B.

doi:10.1016/0014-5793(71)80127-8

Cited by 8 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously examined the evidence for the role of Kurloff cells in the im munology of placentation [9], but the possible involvement of Kurloff cells in immunological processes is still largely speculatvie. Cells having a similar morphology to Kurloff cells have not been demonstrated in spec ies other than the guinea-pig, but the presence of cells in lympho-reticular tissues having a similar function is indicated by the extraction of chemi cally and spectrally similar proteoglycans from the spleens of sheep, pigs, rats and humans [6]. We have shown in the present study that, like KPG.…”

Section: Discussionmentioning

confidence: 54%

“…It possesses a characteristic intracytoplasmic inclusion body [19] which contains a proteoglycan [5], Kurloff cell proteoglycan (KPG) has been extracted from the guinea-pig spleen and purified by D ean and M uir [5] who showed that, although chemically similar to chondroitin 4-sulphate, KPG has an infrared spec trum showing prominent bands absorbing at 805 and 1,260 cm '1 [5,6] in addition to those bands at 720, 850 and 928 cm '1 characteristic of chondroitin 4-sulphate [10]. KPG also shows strong ultraviolet absorp tion with maxima at 257 nm in acid medium and at 265 nm in alkaline medium [6], Proteoglycans possessing chemical and spectral characteris-1 MRC Clinical Research Fellow. …”

mentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of Macrophage Migration by a Proteoglycan Extracted from Kurloff Cells of the Guinea-Pig

Revell¹,

Dean²,

Vernon‐Roberts³

et al. 1972

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Proteoglycan extracted from the Kurloff cells of the guinea-pig spleen and its corresponding glycosaminoglycan inhibits the migration of macrophages from capillary tubes in vitro, but the proteoglycan has no effect on lymphocyte transformation in vitro. A proteoglycan, chemically and spectrally similar to Kurloff cell proteoglycan, extracted from human spleen, also inhibits macrophage migration. In contrast, proteoglycan from cartilage and its corresponding glycosaminoglycan had no significant effect. The differences between Kurloff cell proteoglycan and migration inhibitory factor (MIF) are discussed.

show abstract

Section: Discussionmentioning

confidence: 54%

mentioning

confidence: 99%

Inhibition of Macrophage Migration by a Proteoglycan Extracted from Kurloff Cells of the Guinea-Pig

Revell¹,

Dean²,

Vernon‐Roberts³

et al. 1972

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Kurloff-cell proteoglycan and the corresponding glycosaminoglycan inhibited the migration of macrophages from capillary tubes in vitro at extremelylowconcentrations without effecting transformation of lymphocytes (Revell et al, 1972), whereas proteoglycans and glycosaminoglycans isolated from pig tracheal cartilage by the same procedure had no such effect. Moreover, proteoglycans purified from sheep, pig, rat and human spleens, removed during or immediately after pregnancy, all inhibited macrophage migration and had u.v.-and i.r.-absorption spectra which resembled the spectra of the guinea-pig material (Dean et al, 1971;Revell et al, 1972).…”

mentioning

confidence: 96%

Separation of a proteoglycan fraction from Kurloff cells stimulating protein synthesis in macrophages

Dean¹,

Muir²,

Shires³

1975

Biochemical Journal

View full text Add to dashboard Cite

Proteoglycan from Kurloff cells, when present in the medium in low concentrations, increased the incorporation of (3-H)leucine into trichloracetic acid-precipitable material by macrophages from peritoneal exudates, in addition to inhibiting their migration from capillary tubes, as observed previously. After treatment with 0.5 M-NaOH, followed by dialysis or ultrafiltration, material with the distinctive u.v. and i.r. spectra of the whole proteoglycan appeared in the diffusate, and biological activity was lost from the proteoglycan which remained in the dialysis residue. The diffusible material absorbed near 260 nm and had i.r. bands at 805 cm-minus-1 and 1260 cm-minus-1, but did not display the i.r. bands characteristic of chondrotin 4-sulphate. It contained little sulphate, no hexosamine and less than 1% of the uronic acid present in the whole proteoglycan, and there were only trace amounts of amino acids, xylose and galactose. However, significant amounts of ribose and organic phosphate were present, each representing about 1% of the whole proteoglycan. After proteolysis and chondroitanase digestion of the proteoglycan, a fraction with absorbance at 260 mn was eluted from Dowex 1 with water which stimulated the incorporation of (3H)leucine by macrophages and inhibited their migration from capillary tubes.

show abstract

Peculiar ultraviolet absorbing moieties of human osteoarthritic cartilage

Chayen

Bitensky

Nahir

1990

Cell Biochemistry & Function

View full text Add to dashboard Cite

The ultraviolet absorbing components of human cartilage have been measured by microspectrophotometry. The characteristics of the chondrocytes appeared to be identical, irrespective of the pathology. However the matrix of osteoarthritis cartilage contained components that absorbed maximally in the region of 270 to 250 nm; such components were not found in the matrix of cartilage of non-arthritic joints. Substances that absorb maximally in this region of the ultraviolet could generate free radicals.

show abstract

Proteoglycans from sheep, pig, rat and human spleens having chemical and biological resemblances to that in Kurloff cells

Cited by 8 publications

References 8 publications

Inhibition of Macrophage Migration by a Proteoglycan Extracted from Kurloff Cells of the Guinea-Pig

Inhibition of Macrophage Migration by a Proteoglycan Extracted from Kurloff Cells of the Guinea-Pig

Separation of a proteoglycan fraction from Kurloff cells stimulating protein synthesis in macrophages

Peculiar ultraviolet absorbing moieties of human osteoarthritic cartilage

Contact Info

Product

Resources

About