Proteolytic Activity is Increased in Lymphocytes from Patients with Alzheimer’s Disease

Enantioresolution of dihydropyridine substituted acid by supercritical fluid chromatography on hypercarb® with Z-(L)-arginine as chiral counter ion

“…For this reason we chose a column with smaller ID than the previously used 4.6 mm ID column, which can be run under moderate conditions with respect to flow rate and reasonable retention times even with more strongly retained analytes. The difference in retention could be reduced by partial dynamic deactivation of the support by inclusion of Tween 60 | 174 in the methanol modifier as described earlier in reversed phase LC [18]. The intention of that work was to develop a chromatographic system that made it possible to inject clevidipine dissolved in a complex matrix, Intralipid | [18].…”

“…The difference in retention could be reduced by partial dynamic deactivation of the support by inclusion of Tween 60 | 174 in the methanol modifier as described earlier in reversed phase LC [18]. The intention of that work was to develop a chromatographic system that made it possible to inject clevidipine dissolved in a complex matrix, Intralipid | [18].…”

“…The time course is illustrated in Figure 3, starting with a Tween 60 | deactivated basal system. Equilibration is reached within about 12,000 column volumes after introduction of selector in the methanol Tween 60| This time can be reduced by using a bolus dose of Tween 60 | [18].…”

“…A typical equilibration profile is given in Figure 3. The dip in the curve at 12,000 column volumes was caused by an intermittent increase in the Tween 60 | concentration in order to speed up the equilibration time as described in reversed LC mode [18]. Initially a column temperature of 40 ~ was used but increasing it to 70 ~ reduced retention factors by ca.…”