Proteome analysis of circulating exosomes in health and breast cancer

Тамкович, С. Н.; Bakakina, Y. S.; Tutanov, Oleg; Somov, A. K.; Kirushina, Natalia A.; Dubovskaya, L. V.; Volotovski, I. D.; Laktionov, Pavel P.

doi:10.1134/s1068162017020157

Cited by 6 publications

(8 citation statements)

References 28 publications

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“…To isolate total blood exosomes, a previous protocol was used [12]. Briefly, equal volumes of elution buffer (BioSilica Ltd, Novosibirsk, Russia) were added to blood samples, and incubated on a rotary mixer (10 rpm) for 4 min, at room temperature.…”

Section: Exosome Isolationmentioning

confidence: 99%

“…When exosomes circulate in the blood, they are in contact with blood cells, and after such interactions with cell plasma membranes, some do not undergo immediate membrane fusion/internalization but remain at the cell surface as cell-associated vesicles for some time. Indeed, it has been recently discovered that blood circulating exosomes contain distinguishable fractions of free and cell-surface-associated vesicles [11,12]. The role of blood cell-surface-associated exosomes has not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…The role of blood cell-surface-associated exosomes has not been fully elucidated. Recently, we demonstrated that miRNA and proteins from blood cell-bound exosomes represented valuable sources of materials for cancer diagnostics [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…In the present work, we explored the biological activity of cell-free and blood cell-surface-associated exosomes, materials that were derived from a previous study [12]. We showed that protein cargoes in exosomes from plasma and total blood of healthy females (HFs) and breast cancer patients (BCPs) correlated with redistribution between cell-free and cell-associated fractions of exosomes, and with alterations in the motility characteristics of certain cells.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer Patients Reveals an Association between Different Exosomal Bioactivity on Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro

et al. 2020

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Exosomes are important intercellular communication vehicles, secreted into body fluids by multiple cell types, including tumor cells. They contribute to the metastatic progression of tumor cells through paracrine signalling. It has been recently discovered that blood circulating exosomes contain distinguishable fractions of free and cell-surface-associated vesicles. We evaluated the influence of protein cargoes from exosomes from plasma, and exosomes from the total blood of healthy females (HFs) and breast cancer patients (BCPs), on cell motility. We conducted a mass spectrometric analysis of exosomal contents isolated from samples using ultrafiltration and ultracentrifugation approaches and verified their nature using transmission electron microscopy, nanoparticle tracking analysis and flow cytometry. We observed that malignant neoplasm-associated proteins in exosomes from BCP total blood were detected more often than in plasma (66% vs. 59%). FunRich analysis to assess Gene Ontology (GO) enrichment revealed that proteins with catalytic activities, transporter functions and protein metabolism activities were increased in exosomes from BCP blood. Finally, GO analysis revealed that proteomic profiles of exosomes from HF total blood were enriched with proteins inhibiting cell migration and invasion, which explains the low stimulating activity of exosomes from HF total blood on SKBR-3 cancer cell migration velocity. This allows exosomes to act as intermediaries providing intercellular communications through horizontal transfer of RNA and functionally active proteins, potentially affecting the development of both primary neoplasms and distant metastases.

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Section: Exosome Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer Patients Reveals an Association between Different Exosomal Bioactivity on Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro

et al. 2020

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“…It should be noted that blood circulating exosomes have been reported to contain distinguishable fractions of cell-free and cell-surface-associated vesicles [ 17 , 18 , 19 ]. It is known that circulating blood exosomes contact with blood cell plasma membranes.…”

Section: Introductionmentioning

confidence: 99%

Total Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis

Konoshenko

Sagaradze

Orlova

et al. 2020

IJMS

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Exosomes are crucial players in cell-to-cell communication and are involved in tumorigenesis. There are two fractions of blood circulating exosomes: free and cell-surface-associated. Here, we compared the effect of total blood exosomes (contain plasma exosomes and blood cell-surface-associated exosomes) and plasma exosomes from breast cancer patients (BCPs, n = 43) and healthy females (HFs, n = 35) on crucial steps of tumor progression. Exosomes were isolated by ultrafiltration, followed by ultracentrifugation, and characterized by cryo-electron microscopy (cryo-EM), nanoparticle tracking analysis, and flow cytometry. Cryo-EM revealed a wider spectrum of exosome morphology with lipid bilayers and vesicular internal structures in the HF total blood in comparison with plasma. No differences in the morphology of both exosomes fractions were detected in BCP blood. The plasma exosomes and total blood exosomes of BCPs had different expression levels of tumor-associated miR-92a and miR-25-3p, induced angiogenesis and epithelial-to-mesenchymal transition (EMT), and increased the number of migrating pseudo-normal breast cells and the total migration path length of cancer cells. The multidirectional effects of HF total blood exosomes on tumor dissemination were revealed; they suppress the angiogenesis and total migration path length of MCF10A, but stimulate EMT and increase the number of migrating MCF10A and the total path length of SKBR3 cells. In addition, HF plasma exosomes enhance the metastasis-promoting properties of SKBR3 cells and stimulate angiogenesis. Both cell-free and blood cell-surface-associated exosomes are involved in the crucial stages of carcinogenesis: the initiation of EMT and the stimulation of proliferation, cell migration, and angiogenesis. Thus, for the estimation of the diagnostic/prognostic significance of circulating exosomes in the blood of cancer patients more correctly, the total blood exosomes, which consist of plasma exosomes and blood cell-surface-associated exosomes should be used.

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Identification of Tumor Dissemination Facilitating Proteins in Exosomes Associated with Blood Cells of Breast Cancer Patients

et al. 2020

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Proteome analysis of circulating exosomes in health and breast cancer

Cited by 6 publications

References 28 publications

Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer Patients Reveals an Association between Different Exosomal Bioactivity on Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro

Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer Patients Reveals an Association between Different Exosomal Bioactivity on Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro

Total Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis

Identification of Tumor Dissemination Facilitating Proteins in Exosomes Associated with Blood Cells of Breast Cancer Patients

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