Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

Abstract

Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection.

Proteomic analysis of meropenem-induced outer membrane vesicles released by carbapenem-resistant Klebsiella pneumoniae

“…Hfq is an RNA-binding protein that promotes the interaction between sRNAs and their mRNA targets and is vital for the virulence of K. pneumoniae ( 54 ). Furthermore, PGI, manB, and ompA are virulence-associated proteins according to previous literature studies ( 55 ). OMVs also have specific virulence-related activity.…”

Temporal Patterns of Phenotypic Antimicrobial Resistance and Coinfecting Pathogens in Glaesserella parasuis Strains Isolated from Diseased Swine in Germany from 2006 to 2021

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Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

“…In China, P. nicotianae can cause leaf rot disease of Fatsia japonica [6]. In India and Southern Asia, P. nicotianae is one of the main pathogens among Phytophthora spp., and its hosts include, besides citrus and Nicotiana tabacum, Areca catechu, black pepper, Solanum melongena L., Cocos nucifera L., Durio zibethinus L., Psidium guajava L., orchids, Catharanthus roseus, Ananas comosus, Rosa rugosa, Hevea brasiliensis, and fragrant herbs [7]. World agriculture and forestry have undergone major changes as a conjunction of several factors, including globalization and the intensification of international trade, new farming practices, and climate change [8].…”