Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Zhu, Jia‐Ying; Yè, Gōngyín; Fang, Qi; Hu, Cui

doi:10.1631/jzus.b0820314

Cited by 11 publications

(11 citation statements)

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“…Expression of T. molitor ecCuZnSOD, icCuZnSOD, and MnSOD was upregulated following parasitization by S. guani . This agrees with previous studies that thioredoxin peroxidase, thiol peroxiredoxin, and peroxiredoxin linked to ROS were strongly induced after parasitization (Nguyen et al., ; Zhu et al., ). These antioxidant enzymes reduce the toxic side effects of ROS.…”

Section: Discussionsupporting

confidence: 93%

“…Parasitoids modulate the generation of cytotoxic ROS in their hosts' cellular immune response (Nappi et al, 1995). Although several recent studies have recorded differential expression of genes encoding SODs and other antioxidative proteins in parasitized insects (Nguyen et al, 2008;Zhu et al, 2009Zhu et al, , 2013, studies regarding the role of SODs in host-parasitoid interactions remain underinvestigated.…”

Section: Introductionmentioning

confidence: 99%

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PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR

Zhu

Sang-zi

Stanley

et al. 2014

Arch Insect Biochem Physiol

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Superoxide dismutase (SOD) is an antioxidant enzyme involved in detoxifying reactive oxygen species. In this study, we identified genes encoding the extracellular and intracellular copper-zinc SODs (ecCuZnSOD and icCuZnSOD) and a manganese SOD (MnSOD) in the yellow mealworm beetle, Tenebrio molitor. The cDNAs for ecCuZnSOD, icCuZnSOD, and MnSOD, respectively, encode 24.55, 15.81, and 23.14 kDa polypeptides, which possess structural features typical of other insect SODs. They showed 20-94% identity to other known SOD sequences from Bombyx mori, Musca domestica, Nasonia vitripennis, Pediculus humanus corporis, and Tribolium castaneum. Expression of these genes was analyzed in selected tissues and developmental stages, and following exposure to Escherichia coli and parasitization by Scleroderma guani. We recorded expression of all three SODs in cuticle, fat body, and hemocytes and in the major developmental stages. Relatively higher expressions were detected in late-instar larvae and pupae, compared to other developmental stages. Transcriptional levels were upregulated following bacterial infection. Analysis of pupae parasitized by S. guani revealed that expression of T. molitor SOD genes was significantly induced following parasitization. We infer that these genes act in immune response and in host-parasitoid interactions.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR

Zhu

Sang-zi

Stanley

et al. 2014

Arch Insect Biochem Physiol

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“…Our analysis shows that the transcript levels of SOD, catalase, and peroxiredoxin (belonging to peroxidase) genes were altered after parasitization by S. guani . Moreover, two peroxidases, thiol peroxiredoxin and peroxiredoxin were strongly induced in the plasma of Papilio xuthus after parasitization [72]. However, the expression change may cause perturbation in normal SOD and peroxiredoxin function, which would lead to the production of parasitoid egg or offspring killing by cytotoxic ROS.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Immune Response of Tenebrio molitor Pupae to Parasitization by Scleroderma guani

et al. 2013

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BackgroundHost and parasitoid interaction is one of the most fascinating relationships of insects, which is currently receiving an increasing interest. Understanding the mechanisms evolved by the parasitoids to evade or suppress the host immune system is important for dissecting this interaction, while it was still poorly known. In order to gain insight into the immune response of Tenebrio molitor to parasitization by Scleroderma guani, the transcriptome of T. molitor pupae was sequenced with focus on immune-related gene, and the non-parasitized and parasitized T. molitor pupae were analyzed by digital gene expression (DGE) analysis with special emphasis on parasitoid-induced immune-related genes using Illumina sequencing.Methodology/Principal FindingsIn a single run, 264,698 raw reads were obtained. De novo assembly generated 71,514 unigenes with mean length of 424 bp. Of those unigenes, 37,373 (52.26%) showed similarity to the known proteins in the NCBI nr database. Via analysis of the transcriptome data in depth, 430 unigenes related to immunity were identified. DGE analysis revealed that parasitization by S. guani had considerable impacts on the transcriptome profile of T. molitor pupae, as indicated by the significant up- or down-regulation of 3,431 parasitism-responsive transcripts. The expression of a total of 74 unigenes involved in immune response of T. molitor was significantly altered after parasitization.Conclusions/Significanceobtained T. molitor transcriptome, in addition to establishing a fundamental resource for further research on functional genomics, has allowed the discovery of a large group of immune genes that might provide a meaningful framework to better understand the immune response in this species and other beetles. The DGE profiling data provides comprehensive T. molitor immune gene expression information at the transcriptional level following parasitization, and sheds valuable light on the molecular understanding of the host-parasitoid interaction.

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“…SOD also plays a role in innate immune responses and facilitates resistance to various stresses in many invertebrates, including insects (Zhu, Ze, Stanley, & Yang, ). Changes in the levels of SOD and other antioxidant genes following parasitism have been reported (Nguyen, Boudreault, Michaud, & Cloutier, ; Zhu, Yang, Zhang, Wu, & Yang, ; Zhu, Ye, Fang, & Hu, ). In the present study, envenomation of both feeding and wandering larvae as well as starvation of feeding larvae resulted in the downregulation of ProPO, SOD, and Tpx.…”

Section: Discussionmentioning

confidence: 98%

Induction of stress‐ and immune‐associated genes in the Indian meal moth Plodia interpunctella against envenomation by the ectoparasitoid Bracon hebetor

Shafeeq

UlAbdin

Lee

2017

Arch Insect Biochem Physiol

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Envenomation is an important process in parasitism by parasitic wasps; it suppresses the immune and development of host insects. However, the molecular mechanisms of host responses to envenomation are not yet clear. This study aimed to determine the transcription-level responses of the Indian meal moth Plodia interpunctella against envenomation of the ectoparasitoid Bracon hebetor. Quantitative real-time reverse-transcription PCR was used to determine the transcriptional changes of 13 selected genes, which are associated with development, metabolism, stress, or immunity, in the feeding and wandering fifth instar larvae over a 4-day period after envenomation. The effects of envenomation on the feeding-stage larvae were compared with those of starvation in the transcriptional levels of the 13 genes. Most selected genes were altered in their expression by either envenomation or starvation. In particular, a heat shock protein, hsp70, was highly upregulated in envenomated larvae in both the feeding and wandering stages as well as in starved larvae. Further, some genes were upregulated by envenomation in a stage-specific manner. For example, hsp25 was upregulated after envenomation in the feeding larvae, but hsp90 and an immune-associated gene, hemolin, were upregulated in the wandering larvae. However, both envenomation and starvation resulted in the downregulation of genes associated with development and metabolism. Taken together, P. interpunctella upregulated stress- and immune-responsive genes, but downregulated genes associated with development and metabolism after envenomation. This study provides important information for understanding the molecular mechanisms of host responses to parasitism.

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Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Cited by 11 publications

References 54 publications

PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR

PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR

Transcriptomic Immune Response of Tenebrio molitor Pupae to Parasitization by Scleroderma guani

Induction of stress‐ and immune‐associated genes in the Indian meal moth Plodia interpunctella against envenomation by the ectoparasitoid Bracon hebetor

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