Proteome profiles of HDL particles of patients with chronic heart failure are associated with immune response and also include bacteria proteins

Oberbach, Andreas; Adams, Volker; Schlichting, Nadine; Heinrich, Martina; Kullnick, Yvonne; Lehmann, Stefanie; Lehmann, Sven; Feder, Stefan; Correia, João Carlos; Mohr, Friedrich–Wilhelm; Völker, Uwe; Jehmlich, Nico

doi:10.1016/j.cca.2015.12.005

Cited by 26 publications

(25 citation statements)

References 47 publications

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“…However, the HDL they isolated by ultracentrifugation and used for additional purification contained only 26 proteins, suggesting that their MS analysis lacked adequate sensitivity or that their extensive isolation procedure caused the loss of proteins from the HDL preparation. In contrast to Holzer et al’s work, Oberbach et al [45] recently identified 494 distinct proteins in HDL from five controls and five chronic heart failure patients. Those investigators isolated HDL by sequential ultracentrifugation.…”

Section: Defining Proteins That Associate With Hdlmentioning

confidence: 84%

Quantifying HDL proteins by mass spectrometry: how many proteins are there and what are their functions?

Shao

Heinecke

2017

Expert Review of Proteomics

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Introduction Many lines of evidence indicate that low levels of HDL cholesterol increase the risk of cardiovascular disease (CVD). However, recent clinical studies of statin-treated subjects with established atherosclerosis cast doubt on the hypothesis that elevating HDL cholesterol levels reduces CVD risk. Areas covered It is critical to identify new HDL metrics that capture HDL’s proposed cardioprotective effects. One promising approach is quantitative MS/MS-based HDL proteomics. This article focuses on recent studies of the feasibility and challenges of using this strategy in translational studies. It also discusses how lipid-lowering therapy and renal disease alter HDL’s functions and proteome, and how HDL might serve as a platform for binding proteins with specific functional properties. Expert commentary It is clear that HDL has a diverse protein cargo and that its functions extend well beyond its classic role in lipid transport and reverse cholesterol transport. MS/MS analysis has demonstrated that HDL might contain >80 different proteins. Key challenges are demonstrating that these proteins truly associate with HDL, are functionally important, and that MS-based HDL proteomics can reproducibly detect biomarkers in translational studies of disease risk.

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Section: Defining Proteins That Associate With Hdlmentioning

confidence: 84%

Quantifying HDL proteins by mass spectrometry: how many proteins are there and what are their functions?

Shao

Heinecke

2017

Expert Review of Proteomics

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Section: Discussionmentioning

confidence: 99%

“…Previous studies investigating the HDL proteome in HF observed differences in proteome profiles between healthy subjects and HF patients and proteome profiles in HF patients associated with immune response. 11,12 Additionally, diminished anti-inflammatory, 13 antioxidant activity, 13 -15 and cholesterol efflux capacity 16 in HF in a larger, well-characterised HF patient population with well-matched subgroups, utilising state-of-the-art nanoscale liquid chromatography-coupled mass spectrometry and powerful informatics to analyse the data. Out of 49 HDL proteins that significantly differed between deaths and survivors, we selected 12 proteins to predict death based on multiple statistical approaches.…”

Section: Discussionmentioning

confidence: 99%

Proteomic diversity of high‐density lipoprotein explains its association with clinical outcome in patients with heart failure

Emmens

Jones

Cao

et al. 2017

European J of Heart Fail

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“…Accumulating evidence suggests that HDL function and composition are found to be altered in the presence of overt cardiovascular disease. As such, the presence of CAD, MI [82], heart failure [83], and stroke [84] have been associated with impaired HDL anti-atherogenic properties (i.e., stimulation of vascular NO production, anti-oxidative and anti-apoptotic potential, and cholesterol efflux capacity) [43,85]. In addition, we cannot forget that clinical trials (particularly phase III) include elderly patients under multiple drug treatments.…”

Section: Impact Of Cardiovascular Risk Factor and Co-morbidities On Hmentioning

confidence: 99%

Advances in HDL: Much More than Lipid Transporters

Ben‐Aicha

Badimon

Vilahur

2020

IJMS

107

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High Density Lipoprotein (HDL) particles, beyond serving as lipid transporters and playing a key role in reverse cholesterol transport, carry a highly variable number of proteins, micro-RNAs, vitamins, and hormones, which endow them with the ability to mediate a plethora of cellular and molecular mechanisms that promote cardiovascular health. It is becoming increasingly evident, however, that the presence of cardiovascular risk factors and co-morbidities alters HDLs cargo and protective functions. This concept has led to the notion that metrics other than HDL-cholesterol levels, such as HDL functionality and composition, may better capture HDL cardiovascular protection. On the other hand, the potential of HDL as natural delivery carriers has also fostered the design of engineered HDL-mimetics aiming to improve HDL efficacy or as drug-delivery agents with therapeutic potential. In this paper, we first provide an overview of the molecules known to be transported by HDL particles and mainly discuss their functions in the cardiovascular system. Second, we describe the impact of cardiovascular risk factors and co-morbidities on HDL remodeling. Finally, we review the currently developed HDL-based approaches.

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Proteome profiles of HDL particles of patients with chronic heart failure are associated with immune response and also include bacteria proteins

Cited by 26 publications

References 47 publications

Quantifying HDL proteins by mass spectrometry: how many proteins are there and what are their functions?

Quantifying HDL proteins by mass spectrometry: how many proteins are there and what are their functions?

Proteomic diversity of high‐density lipoprotein explains its association with clinical outcome in patients with heart failure

Advances in HDL: Much More than Lipid Transporters

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