Proteome wide identification of iron binding proteins of Xanthomonas translucens pv. undulosa: focus on secretory virulent proteins

Abstract

Xanthomonas translucens pv. undulosa (Xtu) causes Bacterial Leaf Streak disease in the staple food crops such as wheat and barley. The survival strategies of pathogen and host are determined by the complex interactions occurring between the host plants and the pathogenic microbes. Iron binding proteins are important in the plant-microbe interactions as they are indulged in enzyme catalysis, virulence, metabolic and transport activities. In the presented study, we have identified that ~9.8% of Xtu proteome possess iron binding sequence motifs. Further, the analysis of Xtu proteome for secretory iron binding virulent proteins (IBVPs) revealed the fact that iron co-regulate the function of secretory proteins in virulence. We have found 26 secretory IBVPs and observed that these proteins are diverse in their biological functions ranging from transport to antimicrobial resistance, Reactive oxygen species detoxification and carbohydrate catabolism. The inferences may instigate to design the new strategies to combat and control the microbial diseases of staple food crops.

Targeting metabolic pathways proteins of Orientia tsutsugamushi using combined hierarchical approach to combat scrub typhus

“…Furthermore, the 3D‐structure modeling of the probable therapeutic target was performed using Protein Homology/analogY Recognition Engine v2.0 (Phyre2) program . Template modeled structure with confidence score of >90% and query coverage >50% were selected . The stereochemical properties of the modeled 3D‐structure were checked by constructing Ramachandran plot in PROCHECK .…”

“…41 Template modeled structure with confidence score of >90% and query coverage >50% were selected. 23 The stereochemical properties of the modeled 3D-structure were checked by constructing Ramachandran plot in PROCHECK. 42 The Cofactor server 43 was further employed to find out active site of the protein structure.…”

“…The complete genome sequences of human pathogen Ott strain Ikeda are publicly available and are pivotal for obtaining biological information about this pathogen. The computational analysis of the genome and extraction of information from them is one of the convenient, fast, and time saving strategies . The profound interpretation of metabolic pathways unique to pathogen can help us to understand the factors responsible for its survival and pathogenesis, which can further expedite the identification of novel drug targets.…”

“…The computational analysis of the genome and extraction of information from them is one of the convenient, fast, and time saving strategies. [21][22][23][24] The profound interpretation of metabolic pathways unique to pathogen can help us to understand the factors responsible for its survival and pathogenesis, which can further expedite the identification of novel drug targets. This study reports in silico mining of unique metabolic pathways proteins from Ott strain Ikeda for identification of potential therapeutic targets.…”

In silico Study of Iron, Zinc and Copper Binding Proteins of Pseudomonas syringae pv. lapsa: Emphasis on Secreted Metalloproteins

“…The numerous attempts have been made to biofortify it for mineral micronutrients, such as iron and zinc ( Verma et al, 2016a , b ). The wheat plant and its bacterial pathogen compete for these micronutrients ( Sharma et al, 2017 ). A number of virulence determinants which contribute in the pathogenesis of phytobacteria are extracellular polysaccharides, flagella, secreted proteins, phytohormones, phytotoxin, multidrug efflux pumps and detoxification enzymes ( Ichinose et al, 2013 ).…”