Proteomic analysis by iTRAQ in red claw crayfish, Cherax quadricarinatus, hematopoietic tissue cells post white spot syndrome virus infection

Jeswin, Joseph; Xie, Xiaolu; Ji, Qiao-lin; Wang, Kejian; Liu, Haipeng

doi:10.1016/j.fsi.2016.01.035

Cited by 35 publications

(10 citation statements)

References 62 publications

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“…The hemocytes are the main site in crustaceans where the immune defense is mounted. Proteomics have been widely used to investigate the molecular mechanisms underlying the immune response to WSSV infection in crustaceans but there is no research available on V. alginolyticus infection yet ( 11 , 12 ). Currently an isobaric tag-based methodology for peptide relative quantification [isobaric tags for relative and absolute quantitation (iTRAQ)] coupled to multidimensional liquid chromatography and tandem mass spectrometry enables the assessment of protein levels where four samples can be compared for their common effects ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus

Sun¹,

Wang²,

Wang³

et al. 2017

Front. Immunol.

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In this study, we investigated the hemocytes’ immune response to white spot syndrome virus (WSSV) or Vibrio alginolyticus infection at the protein level. The differential proteomes from crab hemocytes infected with WSSV or V. alginolyticus were analyzed using the isobaric tags for relative and absolute quantitation approach immediately after infection. Using this approach, we identified 1,799 proteins by their by LC–MS/MS spectra and sequencing data. These included 157 upregulated proteins and 164 downregulated proteins after WSSV infection. Similarly, 243 proteins were determined to be differentially expressed during V. alginolyticus infection, of these, 121 were upregulated and 122 were downregulated after infection. Interestingly, among these differentially expressed proteins, 106 were up- or downregulated significantly in both WSSV and V. alginolyticus infection. Six genes, β-actin, myosin-9, anti-lipopolysaccharide factor isoform 4, anti-lipopolysaccharide factor 4, transketolase-like protein 2-like isoform 1, and sarcoplasmic calcium-binding protein 1 were chosen for further study. The expression of these genes all showed a trend of upregulation at 24 h post-WSSV or V. alginolyticus infection except for myosin-9 in response to WSSV. To confirm the protective effects of the six genes, crabs were injected with specific dsRNAs before WSSV or V. alginolyticus challenge. The results showed that the knockdown of these genes led to an increase in the morbidity and mortality (P < 0.01) rate, and a decrease in infection time in WSSV-infected crabs. During the first 84 h, knockdown of these genes also led to an increase in the morbidity rates in V. alginolyticus -infected crabs, and results of four genes showed a higher mortality rate than that of the control after they were knocked down. This is the first report of the proteome response in crab hemocytes during WSSV or V. alginolyticus infection. These findings will contribute to our understanding of the immune response to WSSV and V. alginolyticus infection in crabs.

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Section: Introductionmentioning

confidence: 99%

A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus

Sun¹,

Wang²,

Wang³

et al. 2017

Front. Immunol.

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“…The digested peptides were labeled using iTRAQ reagents (iTRAQ ® Reagents-8plex kit, Sigma Chemical Co., St. Louis, MO, USA) [ 65 , 66 , 67 ]. The labeled samples were pooled and purified using SCX chromatography on an Agilent 1260 HPLC (Agilent Technologies Inc., Santa Clara, CA, USA) [ 68 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomics Reveals the Difference in Root Cold Resistance between Vitis. riparia × V. labrusca and Cabernet Sauvignon in Response to Freezing Temperature

Chen

Xing

et al. 2022

Plants

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Grapevines, bearing fruit containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. However, the cold damage incurred when grown outside in extremely low temperatures during the overwintering stage limits the expansion of production. Although the morphological, biochemical, and molecular levels in different Vitis species exposed to different temperatures have been investigated, differential expression of proteins in roots is still limited. Here, the roots of cold-resistant (Vitis. riparia × V. labrusca, T1) and cold-sensitive varieties (Cabernet Sauvignon, T3) at −4 °C, and also at −15 °C for the former (T2), were measured by iTRAQ-based proteomic analysis. Expression levels of genes encoding candidate proteins were validated by qRT-PCR, and the root activities during different treatments were determined using a triphenyl tetrazolium chloride method. The results show that the root activity of the cold-resistant variety was greater than that of the cold-sensitive variety, and it declined with the decrease in temperature. A total of 25 proteins were differentially co-expressed in T2 vs. T1 and T1 vs. T3, and these proteins were involved in stress response, bio-signaling, metabolism, energy, and translation. The relative expression levels of the 13 selected genes were consistent with their fold-change values of proteins. The signature translation patterns for the roots during spatio-temporal treatments of different varieties at different temperatures provide insight into the differential mechanisms of cold resistance of grapevine.

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“…The main viral pathogens that cause diseases in crustaceans include WSSV, 31,32 yellow-head virus, 33 hepatopancreatic parvo-like virus, 34,35 infectious hypodermal and haematopoietic necrosis virus (IHHNV) 36,37 and monodon baculovirus. [38][39][40] Besides the pathological effects of these viruses, studies using genomics, [41][42][43] transcriptomics 44,45 and proteomics [46][47][48] vate and lactic acid) were found. 52 Similarly, elevated levels of some key metabolites of glycolysis (glucose), nucleotide metabolism (uracil and inosine), amino acid biosynthesis (alanine, tryptophan, histidine, tyrosine, methionine, valine, leucine and isoleucine), TCA cycle (fumaric acid) and lipid metabolism have been found in gills of WSSVinfected crayfish Cherax quadricarinatus.…”

Section: Viral-induced Metabolic Changes In Crustaceansmentioning

confidence: 99%

“…The main viral pathogens that cause diseases in crustaceans include WSSV, 31,32 yellow‐head virus, 33 hepatopancreatic parvo‐like virus, 34,35 infectious hypodermal and haematopoietic necrosis virus (IHHNV) 36,37 and monodon baculovirus 38–40 . Besides the pathological effects of these viruses, studies using genomics, 41–43 transcriptomics 44,45 and proteomics 46–48 have revealed that viral infections cause metabolic changes in crustaceans. For instance, the voltage‐dependent anion channel (VDAC), which controls the influx and efflux of metabolites and ions through the mitochondrial membrane to drive cell energy metabolism, plays a pivotal role in WSSV infection in various crustaceans such as penaeid shrimp P .…”

Section: Changes In Host Metabolomementioning

confidence: 99%

Metabolic reprogramming in crustaceans: A vital immune and environmental response strategy

Wang

Zhang

Yao

et al. 2021

Reviews in Aquaculture

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Most host organisms undergo metabolic changes in response to pathogens, environmental cues or simply to boost their immunity. Metabolic modulation has therefore been exploited by both host and pathogens to outwit the other during host–pathogen interactions. Recent studies have revealed that a growing number of metabolites and metabolic processes in crustaceans are crucial in host–pathogen interactions. Here, we reviewed recent work on metabolic changes during immune response of crustaceans and the metabolic reprogramming that takes place in the host induced by pathogens, environmental cues or as a host strategy to withstand these changes or clear the pathogen. In aquaculture crustaceans, limited studies exist on this subject; hence, we will also highlight themes for possible future research on metabolite reprogramming and how this strategy can be leveraged to improve sustainable aquaculture and enhance crustaceans’ response to pathogens and environmental changes.

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Proteomic analysis by iTRAQ in red claw crayfish, Cherax quadricarinatus, hematopoietic tissue cells post white spot syndrome virus infection

Cited by 35 publications

References 62 publications

A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus

A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus

Comparative Proteomics Reveals the Difference in Root Cold Resistance between Vitis. riparia × V. labrusca and Cabernet Sauvignon in Response to Freezing Temperature

Metabolic reprogramming in crustaceans: A vital immune and environmental response strategy

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