Proteomic Analysis of Bacterial Expression Profiles Following Exposure to Organic Solvent Flower Extract of &lt;i&gt;Melastoma candidu&lt;/i&gt;m D Don (Melastomataceae)

Wong, Fai-Chu; Yong, A-L; Sim, K-M; Ong, H-C; Chai, Tsun-Thai

doi:10.4314/tjpr.v13i7.11

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…Alternatively, secondary or tertiary structures would be equally probable. It should be noted that m / z 129 [M+ • ] has been previously reported in a methanolic plant extract analyzed by GC‐MS, therefore our observation of m / z 130.1595 [M+H + ] was not deemed a laboratory contaminant . Lastly, the lobeline‐like molecules, peaks 5–11 (see Table and Supporting Information ), also displayed a generally increasing trend.…”

Section: Resultssupporting

confidence: 57%

Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures

et al. 2019

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Application of a microfluidic CE* device for CZE‐MS allows for fast, rapid, and in‐depth analysis of large sample sets. This microfluidic CZE‐MS device, the 908 Devices ZipChip, involves minimal sample preparation and is ideal for small cation analytes, such as alkaloids. Here, we evaluated the microfluidic device for the analysis of alkaloids from Lobelia cardinalis hairy root cultures. Extracts from wild‐type, transgenic, and selected mutant plant cultures were analyzed and data batch processed using the mass spectral processing software MZmine2 and the statistical software Prism 8. In total 139 features were detected as baseline resolved peaks via the MZmine2 software optimized for the electrophoretic separations. Statistically significant differences in the relative abundance of the primary alkaloid lobinaline (C27H34N2), along with several putative “lobinaline‐like” molecules were observed utilizing this approach. Additionally, a method for performing both targeted and untargeted MS/MS experiments using the microfluidic device was developed and evaluated. Coupling data‐processing software with CZE‐MS data acquisition has enabled comprehensive metabolomic profiles from plant cell cultures to be constructed within a single working day.

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Section: Resultssupporting

confidence: 57%

Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures

et al. 2019

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“…In this study, alkaloids and saponins were found to reduce the amounts of high molecular weight Lipopolysaccharide (LPS) in E. coli.LPS is a main outer membrane component of Gram-negative bacteria (65).It causes pathophysiological effects such as fever, leucopenia, leucocytosis and Shwartzman reactivity (65). It is also involved in the attachment to host cells and it is important for the virulence and pathogenesis of many bacterial species, including Pseudomonas aeruginosa, Salmonella species, and Escherichia coli (66)(67)(68).…”

Section: Biofilms Are Formed On Surfaces Of Living Tissues Medical Dmentioning

confidence: 99%

The Effect of Alkaloids, Saponinsand Thymoquinone of Nigella Sativa Seeds on Biofilm Production, Motility, Outer Membrane Proteins and Lipopolysaccharide of Some Bacteria.

Shohayeb¹,

Halawani²

2017

IJAR

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“…[52][53][54] Ellagitannins like ellagic acid and punicalagin were reported to inhibit the promoter of the matrix metallopeptidase-9 (MMP-9) of the THP-1 cell line. 55 Also pomegranate fruit extracts which are rich in ellagitannins reduced the proliferation of the breast cancer MCF-7 cells by the up regulation of 505 genes and the down regulation of other 398 genes. 56 Therefore, the damaging and lethal effects of ellagitannins on the adult worms of schistosoma might be attributed at least in part to the down/upregulation of some vital genes for the parasite.…”

Section: Discussionmentioning

confidence: 99%

In vitro Effects of Punica granatum Ellagitannins on Adult Worms of Schistosoma mansoni

Abozeid

El-Badawy

Mahmoud

et al. 2020

RRTM

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Schistosomiasis ranks second behind malaria in terms of overall morbidity and mortality. We evaluated the lethal effect of Punica granatum ellagitannins, extracted from the fruit rind, placenta and barks of the root and stem, on adult worms of Schistosoma mansoni (S. mansoni) . All four ellagitannins were lethal to S. mansoni adult worms. However, while the rind ellagitannins were the most potent, placental ellagitannins were the least. Rind ellagitannins were capable of killing 40% of adult worms at a concentration of 25µg/mL after 5 days. The killing of 100% of the worms was achievable by rind ellagitannins at a concentration of 50µg/mL after 5 days. The LD 50S of the rind ellagitannins after 96h and 120h were 41.25 µg/mL and 28.73 respectively. Ellagitannins-treated worms suffered from erosions, wrinkles, swellings and losses, degenerations of the surface tubercles and tegument. In addition, ellagitannins induced deformation and degradation of oral and ventral suckers and degenerations in the muscles of worms. Ellagitannins also caused a separation of coupled worms and reduction of their motility. Data obtained suggest that ellagitannins of pomegranate could be considered as a cheap candidate for the treatment of schistosomiasis.

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Proteomic Analysis of Bacterial Expression Profiles Following Exposure to Organic Solvent Flower Extract of <i>Melastoma candidu</i>m D Don (Melastomataceae)

Cited by 7 publications

References 24 publications

Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures

Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures

The Effect of Alkaloids, Saponinsand Thymoquinone of Nigella Sativa Seeds on Biofilm Production, Motility, Outer Membrane Proteins and Lipopolysaccharide of Some Bacteria.

<p>In vitro Effects of <em>Punica granatum</em> Ellagitannins on Adult Worms of <em>Schistosoma mansoni</em></p>

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