Proteomic analysis of plasma samples from patients with acute myocardial infarction identifies haptoglobin as a potential prognostic biomarker

Haas, Benjamin; Serchi, Tommaso; Wagner, Daniel R.; Gilson, Georges; Planchon, Sébastien; Renaut, Jenny; Hoffmann, Lucien; Bohn, Torsten; Devaux, Yvan

doi:10.1016/j.jprot.2011.06.028

Cited by 56 publications

(47 citation statements)

References 30 publications

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“…Technological advances have enabled single-cell analysis to provide information about the heterogeneity of the human bone marrow cell population against which diseased states and response to drug treatment could be compared as a basis of mechanistic studies 78 . Complementing transcriptome profiling, proteomic studies have also been used to investigate changes in serum protein levels and, using this approach, haptoglobin has been identified a possible prognostic biomarker after AMI 79 . Similarly, metabolomics 80 and lipidomics 81 methods, although in their infancy, can be also used to identify novel therapeutic targets, and possibly monitor disease activity.…”

Section: Acute Myocardial Infarctionmentioning

confidence: 99%

Inflammatory processes in cardiovascular disease: a route to targeted therapies

et al. 2016

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Inflammatory processes are firmly established as central to the development and complications of cardiovascular diseases. Elevated levels of inflammatory markers have been shown to be predictive of future cardiovascular events. The specific targeting of these processes in experimental models has been shown to attenuate myocardial and arterial injury, reduce disease progression, and promote healing. However, the translation of these observations and the demonstration of clear efficacy in clinical practice have been disappointing. A major limitation might be that tools currently used to measure ‘inflammation’ are insufficiently precise and do not provide information about disease site, activity, or discriminate between functionally important activation pathways. The challenge, therefore, is to make measures of inflammation that are more meaningful, and which can guide specific targeted therapies. In this Review, we consider the roles of inflammatory processes in the related pathologies of atherosclerosis and acute myocardial infarction (AMI), by providing an evaluation of the known and emerging inflammatory pathways. We highlight contemporary techniques to characterize and quantify inflammation, and consider how they might be used to guide specific treatments. Finally, we discuss emerging opportunities in the field, including current limitations and challenges that are the focus of ongoing study.

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Section: Acute Myocardial Infarctionmentioning

confidence: 99%

Inflammatory processes in cardiovascular disease: a route to targeted therapies

et al. 2016

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“…In the last decade, there have been several efforts to dissect out cardiac mRNA and proteome in a wide array of cardiovascular diseases (McGregor and Dunn, 2006) using different animal models or plasma samples collected from human patients (Seenarain et al, 2010; Haas et al, 2011; Silbiger et al, 2011; Drastichova et al, 2012; Chowdhury et al, 2013; Marshall et al, 2014; Petriz and Franco, 2014). While novel biomarkers were identified from some of these studies (Haas et al, 2011; Silbiger et al, 2011; Chowdhury et al, 2013), authors reported general alterations in the cardiac transcriptome and proteome profile that suggested changes in Ca2+ handling proteins (Seenarain et al, 2010), energy metabolism proteins (Jin et al, 2006; Meng et al, 2009), and mediators of apoptotic signaling (Drastichova et al, 2012; Marshall et al, 2014; Petriz and Franco, 2014). …”

Section: Discussionmentioning

confidence: 99%

Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

et al. 2016

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The ligation of the left anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural, and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we compared mRNA expression of genes that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNAs expressions of nine different signal transduction pathways (cell survival and metabolism signs, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis, and inflammation) in rat left ventricle 1 week after MI generated by both coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed in both MI procedures. Interestingly, mRNA expression levels and miRNA expressions showed strong similarities between both models after MI, with few specificities in each model, activating similar signal transduction pathways. To our knowledge, this is the first comparison of genomic alterations of mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses may contribute with the current knowledge concerning transcriptional and post-transcriptional changes of AB-RF protocol, arising as an alternative and effective MI method that reproduces most changes seem in coronary occlusion.

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“…Studies from the Malmö Preventive Study, Sweden, have shown that elevated plasma levels of haptoglobin are a risk factor for MI [19]. Recently, Devaux's group identified haptoglobin as a potential biomarker of prognosis of heart failure in patients with acute MI [20]. Interestingly, they state that low levels of haptoglobin early post-MI favor heart failure.…”

Section: Discussionmentioning

confidence: 99%

Plasma Fractionation Enriches Post-Myocardial Infarction Samples Prior to Proteomics Analysis

Brás

DeLeon

et al. 2012

International Journal of Proteomics

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Following myocardial infarction (MI), matrix metalloproteinase-9 (MMP-9) levels increase, and MMP-9 deletion improves post-MI remodeling of the left ventricle (LV). We provide here a technical report on plasma-analysis from wild type (WT) and MMP-9 null mice using fractionation and mass-spectrometry-based proteomics. MI was induced by coronary artery ligation in male WT and MMP-9 null mice (4–8 months old; n = 3/genotype). Plasma was collected on days 0 (pre-) and 1 post-MI. Plasma proteins were fractionated and proteins in the lowest (fraction 1) and highest (fraction 12) molecular weight fractions were separated by 1-D SDS-PAGE, digested in-gel with trypsin and analyzed by HPLC-ESI-MS/MS on an Orbitrap Velos. We tried five different fractionation protocols, before reaching an optimized protocol that allowed us to identify over 100 proteins. Serum amyloid A substantially increased post-MI in both genotypes, while alpha-2 macroglobulin increased only in the null samples. In fraction 12, extracellular matrix proteins were observed only post-MI. Interestingly, fibronectin-1, a substrate of MMP-9, was identified at both day 0 and day 1 post-MI in the MMP-9 null mice but was only identified post-MI in the WT mice. In conclusion, plasma fractionation offers an improved depletion-free method to evaluate plasma changes following MI.

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Proteomic analysis of plasma samples from patients with acute myocardial infarction identifies haptoglobin as a potential prognostic biomarker

Cited by 56 publications

References 30 publications

Inflammatory processes in cardiovascular disease: a route to targeted therapies

Inflammatory processes in cardiovascular disease: a route to targeted therapies

Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

Plasma Fractionation Enriches Post-Myocardial Infarction Samples Prior to Proteomics Analysis

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