Proteomic analysis of sheep uterus reveals its role in prolificacy

La, Yongfu; Tang, Jiao; Guo, Xiaofei; Zhang, Liping; Gan, Shangquan; Zhang, Xiaosheng; Zhang, Jinlong; Hu, Wenping; Chu, Mingxing

doi:10.1016/j.jprot.2019.103526

Cited by 17 publications

(12 citation statements)

References 73 publications

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“…In another study using MS-based analysis of FF and ovarian vein serum in the Han sheep breed 42 , a total of eight metabolites (glucose 6-phosphate, glucose 1-phosphate, aspartate, asparagine, glutathione oxidized, cysteine-glutathione disulfide, γ-glutamylglutamine, and 2-hydroxyisobutyrate) were significantly associated with ewe litter size. Another recent metabolomic study using LC–MS/MS revealed that sphingolipid and amino acid metabolism is important for maintaining the uterine environment to increase embryo survival rate 43 . In addition to these studies, there are a few other reports that measured individual metabolites in pregnant sheep 18 – 20 , 22 .…”

Section: Discussionmentioning

confidence: 99%

Predictive blood biomarkers of sheep pregnancy and litter size

Goldansaz

Markus

Plastow

et al. 2022

Sci Rep

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Early detection of sheep pregnancy and the prediction of how many lambs a pregnant ewe delivers affects sheep farmers in a number of ways, most notably with regard to feed management, lambing rate, and sheep/lamb health. The standard practice for direct detection of sheep pregnancy and litter size (PLS) is ultrasonography. However, this approach has a number of limitations. Indirect measurement of PLS using blood biomarkers could offer a simpler, faster and earlier route to PLS detection. Therefore, we undertook a large-scale metabolomics study to identify and validate predictive serum biomarkers of sheep PLS. We conducted a longitudinal experiment that analyzed 131 serum samples over five timepoints (from seven days pre-conception to 70 days post-conception) from six commercial flocks in Alberta and Ontario, Canada. Using LC–MS/MS and NMR, we identified and quantified 107 metabolites in each sample. We also identified three panels of serum metabolite biomarkers that can predict ewe PLS as early as 50 days after breeding. These biomarkers were then validated in separate flocks consisting of 243 animals yielding areas-under-the-receiver-operating-characteristic-curve (AU-ROC) of 0.81–0.93. The identified biomarkers could lead to the development of a simple, low-cost blood test to measure PLS at an early stage of pregnancy, which could help optimize reproductive management on sheep farms.

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Section: Discussionmentioning

confidence: 99%

Predictive blood biomarkers of sheep pregnancy and litter size

Goldansaz

Markus

Plastow

et al. 2022

Sci Rep

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“…Two genes were present in this region, one of which was AP1G1 . Proteomic analysis of sheep uterus has revealed the role of AP1G1 in prolificacy ( La et al, 2020 ). Whether the function of NR5A1 and AP1G1 suggested inherent genetic differences in fertility and prolificacy between the Sudanese thin-tailed Desert Sheep and the Chinese breeds is difficult to say in the absence of appropriate phenotype data.…”

Section: Discussionmentioning

confidence: 99%

Genome Divergence and Dynamics in the Thin-Tailed Desert Sheep From Sudan

Abied

Ahbara²,

Berihulay

et al. 2021

Front. Genet.

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With climate change bound to affect food and feed production, emphasis will shift to resilient and adapted indigenous livestock to sustain animal production. However, indigenous livestock comprise several varieties, strains and ecotypes whose genomes are poorly characterized. Here, we investigated genomic variation in an African thin-tailed Desert Sheep sampled in Sudan, using 600K genotype data generated from 92 individuals representing five ecotypes. We included data from 18 fat-tailed and 45 thin-tailed sheep from China, to investigate shared ancestry and perform comparative genomic analysis. We observed a clear genomic differentiation between the African thin-tailed Desert Sheep and the Chinese thin-tailed and fat-tailed sheep, suggesting a broad genetic structure between the fat-tailed and thin-tailed sheep in general, and that at least two autosomal gene pools comprise the genome profile of the thin-tailed sheep. Further analysis detected two distinct genetic clusters in both the African thin-tailed Desert Sheep and the Chinese thin-tailed sheep, suggesting a fine-scale and complex genome architecture in thin-tailed sheep. Selection signature analysis suggested differences in adaptation, production, reproduction and morphology likely underly the fine-scale genetic structure in the African thin-tailed Desert Sheep. This may need to be considered in designing breeding programs and genome-wide association studies.

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“…These selected DEPs associated with the enrichment BP or KEGG pathways and heat stress proteins ( HSPs ). The PRM and qRT-PCR was used for proteomic data validation in a number of studies ( Ren et al, 2017 ; Deng et al, 2020 ; La et al, 2020 ). Eighteen liver tissues were detected by PRM or qRT-PCR.…”

Section: Methodsmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analyses the cycle chronic heat stress affecting liver proteome in yellow-feather chickens

Zhang

Yang

et al. 2021

Poultry Science

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Heat stress ( HS ) is one of the main environmental factors affecting the efficiency of poultry production. The yellow-feather chickens ( YFC ) as an indigenous strain of chicken is a popular poultry breed in China. Our previous study used the RNA-seq to analyze the gene expression profiles of male YFC under HS and showed that the lipid and energy metabolism pathways are activated in livers of YFC exposed to acute HS (38°C, 4 h and 25°C recovery 2 h). In this study, we used quantitative proteome analysis based on iTRAQ to study the liver response of YFC to cycle chronic HS (38 ± 1°C, 8 h/d, 7 d, CyCHS ). The male YFCs treatment used the CyCHS from 22 to 28 days of age. The liver tissue samples were collected at 28 d old. A total of 39,327 unique peptides matches were detected using iTRAQ analysis and 4,571 proteins exhibited a false discovery rate of 1% or less. Forty-six significant differentially expressed proteins ( DEPs ) were detected in the CyCHS group compared with the control group for the liver samples, including up- and down-regulated DEPs were 18 and 28, respectively. We found that the enriched biological process terms of the DEPs expressed in the liver were related to DNA metabolic process, oxidation-reduction process, oxidative stress and gluconeogenesis. In KEGG pathway analysis. Most of the hepatic DEPs were annotated to glutathione metabolism and TCA cycle in response to CyCHS. The up-regulation of 5 DEPs (GPX1, GSTT1, GSTT1L, RRM2, and LOC100859645) in the glutathione metabolism pathway likely reflects an attempt to deal with oxidative damage by CyCHS. The down-regulation of 3 DEPs (Isocitrate dehydrogenase [ IDH3A ], IDH3B, and phosphoenolpyruvate carboxykinase 1) in the TCA cycle pathway contributes to the regulation mechanism of energy metabolism and probably to cope with the balance of heat production and dissipation during CyCHS in order to adapt to high temperature environments. Our results provide insights into the potential molecular mechanism in heat-induced oxidative stress and energy in YFCs and future studies will investigate the functional genes associated with the response to HS.

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Proteomic analysis of sheep uterus reveals its role in prolificacy

Cited by 17 publications

References 73 publications

Predictive blood biomarkers of sheep pregnancy and litter size

Predictive blood biomarkers of sheep pregnancy and litter size

Genome Divergence and Dynamics in the Thin-Tailed Desert Sheep From Sudan

iTRAQ-based quantitative proteomic analyses the cycle chronic heat stress affecting liver proteome in yellow-feather chickens

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