Proteomic analysis of the effect of triterpenes from Patrinia heterophylla on leukemia K562 cells

Wei, Dongfeng; Wei, Yanxia; Chen, Weidong; Yan, Mingfei; Su, Gang; Yan, Hu; Ma, Yaqiong; Han, Chao; Lü, Yan; Cao, Huiming; Bao, Ying-Cun

doi:10.1016/j.jep.2012.09.043

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…Two-dimensional gel electrophoresis (2-DE) was performed as previously reported [ 13 ]. Briefly, protein samples (2 mg), extracted from untreated controls, TGF-β1 treated cells, and COE-treated cells were subjected to isoelectric focusing using the PROTEAN IEF Cell System (Bio-Rad, USA).…”

Section: Methodsmentioning

confidence: 99%

Research on the efficacy of Celastrus Orbiculatus in suppressing TGF-β1-induced epithelial-mesenchymal transition by inhibiting HSP27 and TNF-α-induced NF-κB/Snail signaling pathway in human gastric adenocarcinoma

Zhu

Liu

Qian

et al. 2014

BMC Complement Altern Med

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BackgroundCelastrus orbiculatus has been used as a folk medicine in China for the treatment of many diseases. In the laboratory, the ethyl acetate extract of Celastrus orbiculatus (COE) displays a wide range of anticancer functions. However, the inhibition of the metastasis mechanism of COE in gastric cancer cells has not been investigated so far.MethodsThe present study was undertaken to determine if the anti-metastasis effect of COE was involved in inhibiting of epithelial–mesenchymal transition (EMT) of human gastric adenocarcinoma SGC-7901 cells. In vitro, a well-established experimental EMT model involving transforming growth factor β1 (TGF-β1) was applied. Viability, invasion and migration, protein and mRNA expression of tumor cells were analyzed by MTT assay, transwell assay, western blot and real-time PCR, respectively. The molecular targets of COE in SGC-7901 cells were investigated by two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF mass spectrometer. Overexpression of heat shock protein 27 (HSP27) was performed by transfected with the recombinant retroviral expression plasmid. In vivo, the anti-metastasis mechanisms of COE in the peritoneal gastric cancer xenograft model was explored and the effect was tested.ResultsThe non-cytostatic concentrations of COE effectively inhibited TGF-β1 induced EMT process in SGC-7901 cells, which is characterized by prevented morphological changes, increased E-cadherin expression and decreased Vimentin, N-cadherin expression. Moreover, COE inhibited invasion and migration induced by TGF-β1. Using a comparative proteomics approach, four proteins were identified as differently expressed, with HSP27 protein being one of the most significantly down-regulated proteins induced by COE. Moreover, the activation of nuclear factor κB (NF-κB)/Snail signaling pathway induced by tumor necrosis factor-α (TNF-α) was also attenuated under the pretreatment of COE. Interestingly, overexpression of HSP27 significantly decreases the inhibitory effect of COE on EMT and the NF-κB/Snail pathway. Furthermore, COE significantly reduced the number of peritoneal metastatic nodules in the peritoneal gastric cancer xenograft model.ConclusionsTaken together, these results suggest that COE inhibits the EMT by suppressing the expression of HSP27, correlating with inhibition of NF-κB/Snail signal pathways in SGC-7901 cells. Based on these results, COE may be considered a novel anti-cancer agent for the treatment of metastasis in gastric cancer.

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Section: Methodsmentioning

confidence: 99%

Research on the efficacy of Celastrus Orbiculatus in suppressing TGF-β1-induced epithelial-mesenchymal transition by inhibiting HSP27 and TNF-α-induced NF-κB/Snail signaling pathway in human gastric adenocarcinoma

Zhu

Liu

Qian

et al. 2014

BMC Complement Altern Med

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“…Proteomic technologies such as 2D gel electrophoresis, MALDI-TOF-MS combined peptide mass fingerprint, and the use of Mascot software are used to elucidate the mechanism of action of triterpenes in leukemia cells. The study showed the successful identification of proteins associated with the antitumor mechanism of TCM [ 129 ]. The mechanisms of action involved in the hepatoprotective effects of a TCM were investigated using a combination of 2D gel electrophoresis, MALDI-TOF/TOF-MS, and the construction of protein-protein interaction networks using bioinformatics tools (STRING).…”

Section: Studying the Mechanism Of Action Of Tcms Using Omic Appromentioning

confidence: 99%

The Use of Omic Technologies Applied to Traditional Chinese Medicine Research

Sánchez-Vidaña

Rajwani

Wong

2017

Evidence-Based Complementary and Alternative Medicine

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Natural products represent one of the most important reservoirs of structural and chemical diversity for the generation of leads in the drug development process. A growing number of researchers have shown interest in the development of drugs based on Chinese herbs. In this review, the use and potential of omic technologies as powerful tools in the modernization of traditional Chinese medicine are discussed. The analytical combination from each omic approach is crucial for understanding the working mechanisms of cells, tissues, organs, and organisms as well as the mechanisms of disease. Gradually, omic approaches have been introduced in every stage of the drug development process to generate high-quality Chinese medicine-based drugs. Finally, the future picture of the use of omic technologies is a promising tool and arena for further improvement in the modernization of traditional Chinese medicine.

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“…Homogeneous polysaccharide-0-1 (SCP-0-1) from Schisandra chinensis induces mitochondrial apoptosis in human hepatocellular liver carcinoma, a mechanism involved in the downregulation of HSP90 and inhibition of Akt pathway ( Chen et al, 2016 ). Patrinia heterophylla , a member of Valerianaceae family, inhibits expression of HSP90α to induce apoptosis in leukemia K562 cells ( Wei et al, 2012 ). Platycodin D is a saponin isolated from Platycodonis radix , which can disrupt Hsp90/Cdc37 co-chaperone interactions without affecting ATPase activity of HSP90 and reduces Akt phosphorylation in human lung cancer cells ( Li et al, 2017 ).…”

Section: Tcm Regulates Expression Of Hspsmentioning

confidence: 99%

Traditional Chinese Medicine Targeting Heat Shock Proteins as Therapeutic Strategy for Heart Failure

Wang

et al. 2022

Front. Pharmacol.

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Heart failure (HF) is the terminal stage of multifarious heart diseases and is responsible for high hospitalization rates and mortality. Pathophysiological mechanisms of HF include cardiac hypertrophy, remodeling and fibrosis resulting from cell death, inflammation and oxidative stress. Heat shock proteins (HSPs) can ameliorate folding of proteins, maintain protein structure and stability upon stress, protect the heart from cardiac dysfunction and ameliorate apoptosis. Traditional Chinese medicine (TCM) regulates expression of HSPs and has beneficial therapeutic effect in HF. In this review, we summarized the function of HSPs in HF and the role of TCM in regulating expression of HSPs. Studying the regulation of HSPs by TCM will provide novel ideas for the study of the mechanism and treatment of HF.

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Proteomic analysis of the effect of triterpenes from Patrinia heterophylla on leukemia K562 cells

Cited by 11 publications

References 31 publications

Research on the efficacy of Celastrus Orbiculatus in suppressing TGF-β1-induced epithelial-mesenchymal transition by inhibiting HSP27 and TNF-α-induced NF-κB/Snail signaling pathway in human gastric adenocarcinoma

Research on the efficacy of Celastrus Orbiculatus in suppressing TGF-β1-induced epithelial-mesenchymal transition by inhibiting HSP27 and TNF-α-induced NF-κB/Snail signaling pathway in human gastric adenocarcinoma

The Use of Omic Technologies Applied to Traditional Chinese Medicine Research

Traditional Chinese Medicine Targeting Heat Shock Proteins as Therapeutic Strategy for Heart Failure

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