Proteomic analysis of the effect of diclazuril on second-generation merozoites of Eimeria tenella

Abstract

Diclazuril has long been used as an effective benzeneacetonitrile anticoccidial for the control of Eimeria tenella that causes coccidiosis. However, the molecular mechanism underlying the anticoccidial effects of diclazuril remains elusive. In this study, a proteomic analysis of the effect of diclazuril on second-generation merozoites of E. tenella was performed. Using two-dimensional gel electrophoresis and real-time quantitative polymerase chain reaction (RT-PCR), 13 target proteins were found to be significantly affected by diclazuril treatment, with 11 of these proteins being identified as annotated proteins from E. tenella or other Apicomplexa parasites. These proteins contribute to various functions, including metabolism, protein synthesis, and host cell invasion. Using RT-PCR, we identified the potential pattern of transcriptional regulation induced by diclazuril, and we suggest some promising targets for the intervention of E. tenella infection.

Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle

“…To date, no exhaustive proteomic analysis of the ROPK library has been published on the developmental stages analyzed in the present study to support our analysis. However, some proteomic data are available on sporozoites [ 15 , 34 , 35 , 36 , 37 ] and merozoites [ 36 , 38 , 39 ]. Previous publications demonstrated that the higher transcription of Etrop75 , Etrop5400 , Etrop5840 , Etrop5905 , Etrop26495 , Etrop27695 , and Etrop28765 observed in merozoites in the present study is confirmed at the protein level [ 36 , 38 ], and the higher transcription of Etrop27700 in sporozoites is consistent with proteomic data [ 15 ].…”

The Influence of Unicoccidum Application on the Quality of Poultry Meat

“…При этом он не вызывает угнетения естественного иммунитета, а механизм его действия до конца не изучен. Наиболее чувствительны к препарату неполовозрелые стадии эндогенного цикла развития кокцидий [12,13,14].…”

Eimeria genomics: Where are we now and where are we going?

“…using the transposome-based Nextera system) or the number of cells required for RNAseq (Gertz et al, 2012;Vollmers et al, 2015), as well as improved host genomic resources to screen out contaminating material, will offer further refinements. Improvements in proteomic technologies now allow validation of transcriptomic data and definition of post-transcriptional variation (Lal et al, 2009;Oakes et al, 2013;Shen et al, 2014b). …”