Proteomic analysis on metastasis-associated proteins of human hepatocellular carcinoma tissues

Song, Haiyan; Liu, Yinkun; Feng, Ju-Tao; Cui, Jiefeng; Dai, Zhi; Zhang, Lijun; Feng, Jiaxuan; Shen, Huali; Tang, Zhao–You

doi:10.1007/s00432-005-0044-x

Cited by 56 publications

(48 citation statements)

References 22 publications

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“…HSP27, which is found abundantly in human serum, is suggested as a potential diagnostic marker in breast cancer [14]. Overexpressed HSP27 has been found in metastatic hepatocarcinoma tissues when compared to non-metastatic tissue [66]. Similar results were also obtained in gastric cancer [57].…”

Section: The Role Of Hsp In Cancersupporting

confidence: 84%

Heat Shock Proteins and Cancer: Plant Based Therapy

Uçar

2015

Heat Shock Proteins

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Cancer is one of the major causes of mortality in the world. Each year approximately 13 million people suffer from cancer disease, and approximately 60 % of them die because of cancer. Besides most of the patients response harmful side effects of chemo-and radiotherapies. Therefore the establishment of new therapeutic strategies for the treatment of cancers will be required. A number of studies have shown that some HSP are induced in specific tumor cells. For example, increased levels of HSP105, HSP90, HSP70, HSP60, HSP27 have been detected in colon cancer, lung cancer, hepatocellular carcinoma, colorectal cancer, and gliomas, respectively. Elevated HSP levels in tumor cells are suggested to be responsible for increased chemotherapy resistance and poor prognosis. Suppression of HSP expressions in cancer cells is a new strategy for the treatment. It is well known that some plant extracts and their flavonoids significantly decrease HSP expression, and induce apoptosis of cancer cells. In addition, using of the HSP inhibitors in association with classical chemotherapy increases the sensitivity of cancer cells to the cytotoxic drugs. Therefore, some plants and their biologically active natural compounds have been investigated for their possible contribution to cancer therapy. The current chapter reviews the role of HSP in different cancer types and suppressing HSP with some natural products.

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Section: The Role Of Hsp In Cancersupporting

confidence: 84%

Heat Shock Proteins and Cancer: Plant Based Therapy

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2015

Heat Shock Proteins

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“…Of these treatments, surgical intervention is the most effective for improving the 5-year survival rate of patients (3). One major obstacle for the optimum treatment of HCC is the high expression of anti-apoptotic genes, which contributes to an extremely poor prognosis (4,5). Therefore, novel or additional therapeutic strategies for HCC are required to circumvent this challenge.…”

Section: Introductionmentioning

confidence: 99%

Epigallocatechin-3-gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non-cancerous liver cells

Zhang

Duan

Owusu

et al. 2014

International Journal of Molecular Medicine

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Abstract. Epigallocatechin-3-gallate (EGCG) is a constituent of green tea and has been associated with anticancer activity. In the present study, the inhibitory effect of EGCG on human hepatocellular cancer cells was examined by cell viability assay, in vitro apoptosis assay and cell cycle analysis. In addition, gene expression was measured to elucidate the molecular mechanisms of action of EGCG by mitochondrial membrane potential (MMP) determination and western blot analysis. We demonstrated that EGCG induced apoptosis, decreased mitochondrial membrane potential and promoted G 0 /G 1 phase cell cycle arrest of HCCLM6 cells but not that of non-cancerous liver cells (HL-7702). The EGCG-induced apoptosis of HCCLM6 cells was associated with a significant decrease in Bcl-2 and NF-κB expression. In addition, the expression of Bax, p53, caspase-9 and caspase-3 increased, and cytochrome c was released. These results suggest that EGCG inhibits the progression of cancer through cytocidal activity and that it is a potential therapeutic compound for hepatocellular carcinoma (HCC).

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“…MHCC97H cells were seeded into 96-well plates at a density of 5x10 4 /well. For the cell inhibition assay, cells were incubated with different concentrations of CIE 200, 400, 800, 1200, 1600 μg/ml or vehicle alone for 24 h, following by a MTT assay as described (23).…”

Section: Preparation Of Chrysanthemum Indicum Extractmentioning

confidence: 99%

“…Features of HCC are an aggressive cancer with a dismal outcome largely due to metastasis and postsurgical recurrence (4). Thus, the inhibition of invasion and metastasis is of great importance in the HCC therapies.…”

Section: Introductionmentioning

confidence: 99%

Chrysanthemum indicum ethanolic extract inhibits invasion of hepatocellular carcinoma via regulation of MMP/TIMP balance as therapeutic target

Wang

Huang²,

Li³

et al. 2009

Oncol Rep

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Abstract. Hepatocellular carcinoma (HCC) is an aggressive cancer with a dismal outcome largely due to metastasis and postsurgical recurrence. Thus, the inhibition of invasion and metastasis is of great importance in its therapies. Medicinal plants or ethnopharmacology used in folklore medicine continue to be an important source of discovery and development of novel or potential therapeutic agents for treatment of cancer. Chrysanthemum indicum, one of the medicinal plants or ethnopharmacology, is being used for treatment of many diseases including cancer. However, this plant molecular mechanisms underlining the anti-metastatic effects have not been well documented. In this study, Chrysanthemum indicum ethanolic extract (CIE) significantly suppressed proliferation and invasion of MHCC97H cells, one of the HCC cell lines with high metastatic potential, in a dose-dependent manner. CIE markedly decreased MMP-2 and MMP-9 expression, increased simultaneously TIMP-1, and TIMP-2 expression further restoring their balance in the cancer cells. The present study indicates that CIE reduced MHCC97H cell metastatic capability, in part at least, through decrease of the MMP expression, simultaneous increase of the TIMP expression, further restoring their balance as therapeutic target in HCC. It is suggested that Chrysanthemum indicum is a potential novel therapeutic medicinal plant for treatment of HCC or cancer invasion and metastasis.

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Proteomic analysis on metastasis-associated proteins of human hepatocellular carcinoma tissues

Abstract: There are various proteins joined together in HCC metastasis. The overexpression of HSP27 may serve as a biomarker for early detection and therapeutic targets unique to the metastatic phenotype of HCC.

Cited by 56 publications

References 22 publications

Heat Shock Proteins and Cancer: Plant Based Therapy

Heat Shock Proteins and Cancer: Plant Based Therapy

Epigallocatechin-3-gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non-cancerous liver cells

Chrysanthemum indicum ethanolic extract inhibits invasion of hepatocellular carcinoma via regulation of MMP/TIMP balance as therapeutic target

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