Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica

Ning, Deli; Lu, Tian-Chong; Yang, Chuanping; Wang, Baichen

doi:10.1007/s11105-013-0593-0

Cited by 10 publications

(9 citation statements)

References 47 publications

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“…The loss of apical dominance in the P. sylvestris var. mongolica was accompanied by a significant decrease in IAA and CK content compared to wild type [ 29 ]. The characteristics of dwarfed variant P. bungeana , short plants and numerous lateral branches, may be closely related to the significant increase in ZT, the decrease in IAA/ABA and the increase in ZT/IAA [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Targeted Metabolic and Transcriptomic Analysis of Pinus yunnanensis var. pygmaea with Loss of Apical Dominance

Xiao

Yang

Wang

et al. 2022

CIMB

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Pinus yunnanensis var. pygmaea demonstrates obvious loss of apical dominance, inconspicuous main trunk, which can be used as an ideal material for dwarfing rootstocks. In order to find out the reasons for the lack of apical dominance of P. pygmaea, endogenous phytohormone content determination by liquid chromatography–tandem mass spectrometry (LC–MS/MS) and comparative transcriptomes were performed on the shoot apical meristem and root apical meristem of three pine species (P. massoniana, P. pygmaea, and P. elliottii). The results showed that the lack of CK and the massive accumulation of ABA and GA-related hormones may be the reasons for the loss of shoot apical dominance and the formation of multi-branching, the abnormal synthesis of diterpenoid biosynthesis may lead to the influence of GA-related synthesis, and the high expression of GA 2-oxidase (GA2ox) gene may be the cause of dwarfing. Weighted correlation network analysis (WGCNA) screened some modules that were highly expressed in the shoot apical meristem of P. pygmaea. These findings provided valuable information for identifying the network regulation of shoot apical dominance loss in P. pygmaea and enhanced the understanding of the molecular mechanism of shoot apical dominance growth differences among Pinus species.

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Section: Discussionmentioning

confidence: 99%

Targeted Metabolic and Transcriptomic Analysis of Pinus yunnanensis var. pygmaea with Loss of Apical Dominance

Xiao

Yang

Wang

et al. 2022

CIMB

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“…Compared with wild-type Pinus sylvestris seedlings, P. sylvestris var. mongolica, a natural dwarf-type mutant, showed decreased accumulation of Rad23 proteins, indicating that these proteins are among the main proteins responsible for the reduction in growth in dwarf-type mutants (Ning et al 2013). These authors suggested that Rad23 proteins may play an important role in signaling cascades involved in the regulation of plant growth, particularly apical dominance.…”

Section: Discussionmentioning

confidence: 99%

“…These authors suggested that Rad23 proteins may play an important role in signaling cascades involved in the regulation of plant growth, particularly apical dominance. Indeed, these proteins may be involved in the auxin-mediated regulation of apical dominance regulating plant growth (Ning et al 2013). Thus, this protein may be important for the proper development of C. ssilis shoots, and the decreased accumulation induced by the D-arg treatment (BA + Put + D-arg) could be related to the lower shoot length observed compared to that under the Put + BA treatment.…”

Section: Discussionmentioning

confidence: 99%

The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile

Aragão

Sousa

Oliveira

et al. 2022

Plant Cell Tiss Organ Cult

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Polyamines (PAs) and proteins have been demonstrated to be fundamental for in vitro shoot development of Cedrela ssilis. We evaluated the in uence of 6-benzyladenine (BA) and putrescine (Put) on the growth of shoots, PA metabolism and proteomic pro les of C. ssilis.The longest shoots were obtained under 2.5 μM BA + 2.5 mM Put treatment. The inhibition of Put synthesis by D-arginine (D-arg) reduced the activities of the Put biosynthesis enzymes arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) and the endogenous contents of free Put, resulting the reduced shoot growth. The ODC activity was higher than that ofADC, which is the main enzyme in the synthesis of Put in C. ssilis. Inhibition of Put synthesis affected the proteomic pro le, reducing the accumulation of the ubiquitin receptor RAD23c, peroxidase 15, ADP-ribosylation factor 1, ADP-ribosylation factor-like protein 8a, pro lin-4, pro lin-2, glucan endo-1,3-beta-glucosidase, and expansin-like B1 and increasing the accumulation of V-type proton ATPase catalytic subunit A and methionine gamma-lyase, highlighting the relevance of these proteins in promoting the length of shoots. Moreover, the transport protein SEC13 homolog B and the basic isoform glucan endo-1,3-beta-glucosidase, unique proteins in shoots treated with BA+Put, were related to the promotion of shoot growth. Our results show that the modulation of endogenous PAs and proteomic pro les is necessary to regulate in vitro morphogenesis in C. ssilis. Moreover, the ODC enzyme is highly involved in the synthesis of Put during in vitro shoot development and is described for the rst time in this species.

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“…RAD23 family members have been identified in budding yeast (S. cerevisiae), humans (Homo sapiens), carrot (Daucus carota), rice (Oryza sativa), Arabidopsis thaliana, pine (Pinus sylvestris var. mongolica ), and maize (Zea mays) (Madura and Prakash, 1990;Masutani et al 1994;Schultz and Quatrano, 1997;Sturm and Lienhard, 1998;Zuo and Mahajan 2005;Farmer et al 2010;Ning et al 2013). Unlike their characterizations in yeast and humans, the functions of RAD23s in plants are less clear.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, RAD23 is required for maintaining apical dominance in Pinus sylvestris var. mongolica (Ning et al 2013). For apple (Malus×domestica Borkh.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of the radiation sensitivity protein-23 (RAD23) family members in apple (Malus×domestica Borkh.) and expression analysis of their stress responsiveness

Wang

Gong

2017

Journal of Integrative Agriculture

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Radiation sensitivity-23 proteins (RAD23) are DNA repair factors participate in the ubiquitin/proteasome system (UPS). Although the genome-wide analysis of RAD23 family members has been conducted in some species, little are known about RAD23 genes in apple (Malus×domestica Borkh.). We analyzed this gene family in M. domestica in terms of genomic locations, protein and promoter structures, and expressions in response to stresses. Various members showed a ubiquitous pattern of expression in all selected apple parts. Their expressions were altered under chilling, heat, and hydrogen peroxide treatments, as well as abscisic acid (ABA) treatment and water deficiency, suggesting their possible roles in plant stress responses. These results provide essential information about RAD23 genes in apple and will contribute to further functional studies.

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Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica

Cited by 10 publications

References 47 publications

Targeted Metabolic and Transcriptomic Analysis of Pinus yunnanensis var. pygmaea with Loss of Apical Dominance

Targeted Metabolic and Transcriptomic Analysis of Pinus yunnanensis var. pygmaea with Loss of Apical Dominance

The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile

Genome-wide identification of the radiation sensitivity protein-23 (RAD23) family members in apple (Malus×domestica Borkh.) and expression analysis of their stress responsiveness

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