Proteomic analysis provides new insight into the chicken eggshell cuticle

Gastrokine-2 is transiently expressed in the endodermal and endothelial cells of the maturing mouse yolk sac

“…In contrast, Gkn2 protein was most found in the basal side of the YS endoderm, whereas F-actin in the YS is concentrated at the inner leaf of the apical cell membrane facing the maternal tissue suggesting that the majority of YS gastrokine-2 is not associated with F-actin. The chicken orthologue of gastrokine-2 was identified in an unbiased proteomics analysis of the egg cuticle (Rose-Martel et al, 2012).…”

Eggshell structure, measurements, and quality-affecting factors in laying hens: a review

“…Cuticle deposition is important for the prevention of micro-organisms penetration, which is a frequent event in the absence of cuticle deposition. This feature is reserved by the antimicrobial substances such as lysozyme and ovotransferrin deposited in eggshell cuticle (Rose-Martel et al 2012;Miksik et al 2014). Additionally, Messens et al (2007), De Reu et al (2010, and Bain et al (2013) observed a high correlation between the absence of cuticle and bacterial penetration across the eggshell.…”

“…Additionally, Messens et al (2007), De Reu et al (2010, and Bain et al (2013) observed a high correlation between the absence of cuticle and bacterial penetration across the eggshell. Cuticle is also important to create a barrier which inhibits water movement across the shell and prevents dehydration of the egg interior components (Rose-Martel et al 2012). Cuticle deposition is affected by laying hen age (Rodriguez-Navarro et al 2013) and housing systems .…”

Dynamics of Structural Barriers and Innate Immune Components during Incubation of the Avian Egg: Critical Interplay between Autonomous Embryonic Development and Maternal Anticipation

“…The ES protein osteopontin has recently been shown to control ES nanostructure, where the dimensions of the calcite mineral structure correlate with ES hardness [97]. The outermost layer of protein-rich cuticle contains antimicrobial proteins such as LYZ [98, 99]. …”

“…This organic matrix contains a number of antibacterial proteins such as LYZ, TF, OCX36, OC17, OCX32, CST3, and the AvBDs [93, 95, 99, 102]. The mechanism of action of these molecules within the ES remains unclear, but it is likely that they are solubilized during limited ES dissolution that occurs during embryonic development and could also provide local protection at the interface between the ES and extra-embryonic structures (Fig.…”