2011
DOI: 10.1097/tp.0b013e31821d262b
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Proteomic Analysis Reveals Innate Immune Activity in Intestinal Transplant Dysfunction

Abstract: Background Many patients with intestinal failure require intestinal transplantation (ITx) to survive. Acute cellular rejection poses a challenge in ITx because its biologic components are incompletely understood. New methodologies for its integrative and longitudinal analysis are needed. Methods In this study, we characterized episodes of acute cellular rejection in ITx recipients using a noninvasive proteomic analysis. Ostomy effluent was obtained from all patients undergoing ITx at University of California… Show more

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Cited by 18 publications
(10 citation statements)
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“…1). Those findings are consistent with results obtained in animal models describing the critical role of IFNdependent chemokines in ITx rejection process (15,16), or in the clinical setting showing the association of IFNG production with ITx rejection, either detected by gene expression analysis of the intestinal mucosa (8,9) or proteomic analysis of intestinal content during ACR episodes (21).…”
Section: Discussionsupporting
confidence: 85%
“…1). Those findings are consistent with results obtained in animal models describing the critical role of IFNdependent chemokines in ITx rejection process (15,16), or in the clinical setting showing the association of IFNG production with ITx rejection, either detected by gene expression analysis of the intestinal mucosa (8,9) or proteomic analysis of intestinal content during ACR episodes (21).…”
Section: Discussionsupporting
confidence: 85%
“…To examine the cross-sectional histology of human mucosa, microtome sections of paraffin tissues were obtained from an independent non-IBD human cohort, and stained by immunohistochemistry with primary antibody and developed by VECTASTAIN Elite ABC Kit (Vector Lab, Burlingame, CA) as previously described. 21 The same antibodies used in immunoblotting also were used in immunohistochemistry (IHC), with the exception that the antihepcidin antibody was replaced by an antiprohepcidin antibody (gifts from Dr Tomas Ganz's laboratory). To examine whole-mounts of intestinal mucosa, 3 cm 2 human intestinal samples were processed as previously described, 22 and reacted with biotin-conjugated primary antibodies using EZ-link Sulfo-NHS-Biotin (Thermo Fisher Scientific).…”
Section: Methodsmentioning
confidence: 99%
“…The CCLs measured in this study were part of two luminex kits (Milliplex Map Human Cytokine/Chemokine Panel II 23‐plex; EMD Millipore, Billerica, MA, USA and Bio‐Plex Pro Human Cytokine 27‐plex; Bio‐Rad Laboratories Canada Ltd, Mississauga, ON, Canada). Analysis was carried out on the Bio‐Plex 200 instrument, and data were analyzed by Bio‐Plex Manager Software (version 6.1; ).…”
Section: Methodsmentioning
confidence: 99%