Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro

Muneer, Sowbiya; Wei, Hao; Park, Yoo Gyeong; Jeong, Hai Kyoung; Jeong, Byoung Ryong

doi:10.3390/ijms19010050

Cited by 11 publications

(3 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The addition of K 2 SiO 3 to the medium at concentrations of 1.8 mM and 3.6 mM slowed down lipid peroxidation and promoted the restoration of stomatal functions. The results of proteomic analysis indicate the active participation of silicon in various metabolic processes aimed at the restoration of hyperhydric shoots [ 25 , 94 ]. It is known that silicon in the medium in the form of potassium, sodium, and calcium silicates improved shoot growth, promoted an increase in biomass, and an increase in the stability of cells, tissues, and organs in in vitro culture [ 95 , 96 , 97 ].…”

Section: Influence Of Medium Composition and Culture Conditions On Hy...mentioning

confidence: 99%

Hyperhydricity in Plant Tissue Culture

Polivanova

Bedarev

2022

Plants

View full text Add to dashboard Cite

Hyperhydricity is the most common physiological disorder in in vitro plant cultivation. It is characterized by certain anatomical, morphological, physiological, and metabolic disturbances. Hyperhydricity significantly complicates the use of cell and tissue culture in research, reduces the efficiency of clonal micropropagation and the quality of seedlings, prevents the adaptation of plants in vivo, and can lead to significant losses of plant material. This review considers the main symptoms and causes of hyperhydricity, such as oxidative stress, impaired nitrogen metabolism, and the imbalance of endogenous hormones. The main factors influencing the level of hyperhydricity of plants in vitro are the mineral and hormonal composition of a medium and cultivation conditions, in particular the aeration of cultivation vessels. Based on these factors, various approaches are proposed to eliminate hyperhydricity, such as varying the mineral and hormonal composition of the medium, the use of exogenous additives, aeration systems, and specific lighting. However, not all methods used are universal in eliminating the symptoms of hyperhydricity. Therefore, the study of hyperhydricity requires a comprehensive approach, and measures aimed at its elimination should be complex and species-specific.

show abstract

Section: Influence Of Medium Composition and Culture Conditions On Hy...mentioning

confidence: 99%

Hyperhydricity in Plant Tissue Culture

Polivanova

Bedarev

2022

Plants

View full text Add to dashboard Cite

show abstract

“…Evans blue staining was used to indicate the membrane damage of cell death [ 36 ]. We examined the production of superoxide anion (O 2 -) and hydrogen peroxide (H 2 O 2 ) in situ by NBT [ 37 ] and 3, 3′-diaminobenzidine (DAB) staining, respectively [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Regulation of Flowering Timing by ABA-NnSnRK1 Signaling Pathway in Lotus

Cao

Jin

Kuang

et al. 2021

IJMS

View full text Add to dashboard Cite

The lotus produces flower buds at each node, yet most of them are aborted because of unfavorable environmental changes and the mechanism remains unclear. In this work, we proposed a potential novel pathway for ABA-mediated flower timing control in the lotus, which was explored by combining molecular, genetic, transcriptomic, biochemical, and pharmacologic approaches. We found that the aborting flower buds experienced extensive programmed cell death (PCD). The hormonal changes between the normal and aborting flower buds were dominated by abscisic acid (ABA). Seedlings treated with increasing concentrations of ABA exhibited a differential alleviating effect on flower bud abortion, with a maximal response at 80 μM. Transcriptome analysis further confirmed the changes of ABA content and the occurrence of PCD, and indicated the importance of PCD-related SNF1-related protein kinase 1 (NnSnRK1). The NnSnRK1-silenced lotus seedlings showed stronger flowering ability, with their flower:leaf ratio increased by 40%. When seedlings were treated with ABA, the expression level and protein kinase activity of NnSnRK1 significantly decreased. The phenotype of NnSnRK1-silenced seedlings could also be enhanced by ABA treatment and reversed by tungstate treatment. These results suggested that the decline of ABA content in lotus flower buds released its repression of NnSnRK1, which then initiated flower bud abortion.

show abstract

“…Silicon hardens the cell walls in the epidermis and cortex of vascular tissues. It also promotes cell turgidity and normal stomatal opening (Muneer et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

In vitro Regeneration, Rooting, and Cloning of Artemisia tridentata

Barron¹

View full text Add to dashboard Cite

Artemisia tridentata (big sagebrush) is an ecologically important shrub found in western North America. In vitro techniques can be applied to big sagebrush for the purpose of studying gene function, genotypic and phenotypic plasticity studies, cloning, genotypic preservation, and restoration. I performed experiments to develop an indirect organogenesis protocol to regenerate whole Wyoming big sagebrush plants from leaf explants. Callus formation frequency was 88% (±4.0%) in leaf explants cultured on medium containing 0.5 mg/l BAP and 1.0 mg/l NAA. Shoot formation frequency was variable between replicates and was the highest when callus tissue was cultured on medium containing 1.5 mg/l BAP and 0.1 mg/l NAA, 37% to 80%. I tested several auxin treatments to induce root formation and concluded the best to be 0.5mg/l IBA, which yielded 42% to 60% rooting. Taking into account all these variables, I estimate the total regeneration efficiency to range between 14% to 43% on this set of treatments. This protocol was also applied to basin big sagebrush. Callus formation was 100% in leaf explants. Shoot formation was 34% (±14.6%), but shoots exhibited a hyperhydric phenotype and were not transferred to root induction medium. The in vitro regeneration protocol developed is a crucial element that would be required to transform big sagebrush using molecular approaches. Experiments were also conducted to determine the feasibility of shoot tip and nodal cuttings to develop adventitious roots in vitro. This method can provide genetically identical material much faster than in vitro regeneration. Adventitious root formation in Wyoming big sagebrush cuttings cultured on two media types was inconsistent, ranging from 10% in some experiments to 80% in others. Limited success was achieved in nodal cuttings cultured on modified MS medium containing auxin and cytokinin 12.5% (±5.6%). No root formation was achieved in mature plant tissue collected in the field. Results indicated that genotypic influences were likely more responsible for variations in rooting than the medium or vessel conditions tested. Cloning experiments in basin big sagebrush further supported this notion. All material for these experiments came from half-sibling individuals that was maintained separately throughout the course of the experiments. Some half-siblings formed no adventitious roots on any treatments tested whereas others had high rates of formation on all treatments. Further studies, utilizing exogenous PGRs, such as auxins, may provide more successful adventitious root formation in shoot tips from both big sagebrush subspecies.

show abstract

Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro

Cited by 11 publications

References 64 publications

Hyperhydricity in Plant Tissue Culture

Hyperhydricity in Plant Tissue Culture

Regulation of Flowering Timing by ABA-NnSnRK1 Signaling Pathway in Lotus

In vitro Regeneration, Rooting, and Cloning of Artemisia tridentata

Contact Info

Product

Resources

About