Proteomic Analysis Reveals the Positive Effect of Exogenous Spermidine in Tomato Seedlings' Response to High-Temperature Stress

Sang, Q. Q.; Xi, Shibo; An, Yueqi; Shu, Sheng; Sun, Jian; Guo, Shirong

doi:10.3389/fpls.2017.00120

Cited by 44 publications

(45 citation statements)

References 56 publications

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“…High temperature disturbs the subtle balance between photosynthesis and respiration; photosynthesis is viewed as a heat-sensitive cell function and process (Yamori et al, 2014). The structure and function of the chloroplasts are disturbed, photosynthetic pigments are degraded, and the photosystem II and the activation state of rubisco are influenced under high temperature (Sang et al, 2017;Sharwood et al, 2017). Leaves lose their green color and are not able to perform photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Relative Air Humidity and High Temperature on the Physiological and Anatomical Responses of Two Rhododendron Cultivars

Ma¹,

Liang²,

Zhao³

2019

horts

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The effects of different relative air humidity (RH) levels under high temperature and accompanying vapor pressure deficits (VPDs) on the physiology, photosynthesis, and anatomy of Rhododendron need to be better understood to help in reducing damage to leaves caused by high temperature. In this study, two Rhododendron cultivars were exposed at 45%, 55%, 65%, 75%, and 85% RH to a treatment of constant temperature at 38 °C for 14 days, resulting in a VPD of 3.64, 2.98, 2.32, 1.66, and 0.99 kPa, respectively. The results showed the least reduction of the net photosynthetic rate (Pn) under 75% RH treatment in R. ‘Fen Zhenzhu’ (decreased by 79.8%), and under the 85% RH treatment in R. ‘Zhuangyuan Hong’ (decreased by 75.4%). The decline in relative water content (RWC) was less under the 75% and 85% RH treatments, and electrolyte leakage showed a slight decrease under the 75% RH treatment in the two Rhododendron cultivars. The appearance of the two cultivars under greater RH showed less damage, probably because plants can avoid damage by increasing total chlorophyll content, decreasing stomatal area, stomatal density, and opened stomata ratio, enhancing enzymatic activity and osmoregulation substances, and improving leaf structure. The findings show that greater RH can alleviate damage caused by heat stress and improve thermostability.

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Section: Discussionmentioning

confidence: 99%

Effect of Relative Air Humidity and High Temperature on the Physiological and Anatomical Responses of Two Rhododendron Cultivars

Ma¹,

Liang²,

Zhao³

2019

horts

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“…For example, FcWRKY70 positively regulated Put synthesis to confer drought tolerance in Fortunella crassifolia [ 19 ]. Spd promotes biomass accumulation and upregulates proteins involved in cell rescue and defense as well as antioxidant enzymes in tomato ( Lycopersicon esculentum ) seedlings under high-temperature stress [ 20 ]. Exogenous Spm was proved to induce defense response and increase resistance to root rot pathogen Phytophthora capsici in Capsicum annuum plants [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Melatonin Alleviates Alkaline Stress in Malus hupehensis Rehd. by Regulating the Biosynthesis of Polyamines

et al. 2017

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Since melatonin was identified in plants decades ago, much attention has been devoted to discovering its role in plant science. There is still a great deal to learn about the functional importance of melatonin, as well as its functional mode. In this paper, we examine the role of melatonin treatment in the response of Malus hupehensis Rehd. to alkaline conditions. Stressed seedlings showed chlorosis and suppressed growth. However, this phenotype was ameliorated when 5 µM melatonin was added to the irrigation solution. This supplementation was also associated with a reduction in cell membrane damage and maintenance of a normal root system architecture. Fewer reactive oxygen species (ROS) were accumulated due to the enhanced scavenging activity of antioxidant enzymes superoxide dismutase, peroxidase, and catalase. In addition, alkaline-stressed seedlings that received the melatonin supplement accumulated more polyamines compared with untreated seedlings. Transcript levels of six genes involved in polyamine synthesis, including SAMDC1, -3, and -4, and SPDS1, -3, and -5, -6, were upregulated in response to melatonin application. All of these results demonstrate that melatonin has a positive function in plant tolerance to alkaline stress because it regulates enzyme activity and the biosynthesis of polyamines.

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“…Rubisco is a major photosynthetic protein, and it is also involved in the photorespiratory pathway in plants. Importantly, it is conceived as the key limiting factor of net photosynthesis under stress (Ahsan et al 2007b, Sang et al 2017. Down-regulation of the Rubisco-binding protein has been previously reported under flooded conditions (Stoychev et al 2013).…”

Section: Alteration In Leaf Proteome Under Waterlogging Stressmentioning

confidence: 96%

Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stages

Jung¹,

Roy²,

Cho³

et al. 2019

Biologia plant.

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Waterlogging, a major environmental stress, impairs plant growth and development and induces synthesis of different proteins. To understand the molecular mechanisms coupled with morpho-physiological alterations underlying waterlogging tolerance, the LTQ-FTICR MS/MS technique was employed to map the proteomes of leaves of sesame grown under control and waterlogged conditions. The waterlogging treatment caused dramatic alterations in morphological and biochemical properties of the leaves of sesame. For proteome analysis, more than 75 reproducible protein spots were identified on 2-DE gels wherein 51 protein spots (≥ 1.5-fold change) were used for analysis by mass spectrometry. Among 51 differentially abundant proteins, 20 were specific to the 10-leaf stage and 31 were specific to the flowering stage. Most of the differentially abundant proteins were involved in group metabolism, and energy and stress defense. Oxygen-evolving enhancer protein 1, ATP synthase subunit, heat shock proteins, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase, and superoxide dismutase were upregulated under waterlogging. However, the photosynthesis-and protein biosynthesisrelated proteins (e.g., ribulose-1,5-bisphosphate carboxylase/oxygenase activase, and S-adenosylmethionine synthase 1) were down-regulated under waterlogging. The protein interaction network indicates that energy metabolism-and stressand defense-related proteins were involved in the protein-protein interaction network, which could form an indispensable network in sesame leaves. To this end, physiological results highlighted the impairment of photosyntheis, which is consistent with results obtained at the proteome level. The upregulation of metabolism-, energy-, and stress defenserelated proteins in response to waterlogging stress may provide new insights into the complex mechanisms underlying waterlogging tolerance in sesame.

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Proteomic Analysis Reveals the Positive Effect of Exogenous Spermidine in Tomato Seedlings' Response to High-Temperature Stress

Cited by 44 publications

References 56 publications

Effect of Relative Air Humidity and High Temperature on the Physiological and Anatomical Responses of Two Rhododendron Cultivars

Effect of Relative Air Humidity and High Temperature on the Physiological and Anatomical Responses of Two Rhododendron Cultivars

Exogenous Melatonin Alleviates Alkaline Stress in Malus hupehensis Rehd. by Regulating the Biosynthesis of Polyamines

Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stages

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