Proteomic analysis to display the effect of low doses of erythropoietin on rat liver regeneration

Bader, Augustinus; Pavlica, Sanja; Deiwick, Andrea; Lotková, Halka; Kučera, Otto; Darsow, Kai; Bartel, Sebastian; Schulze, Mattes; Lange, Harald A.; Červinková, Z

doi:10.1016/j.lfs.2011.08.002

Cited by 17 publications

(9 citation statements)

References 40 publications

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“…The immunohistochemical analysis of bromo de oxyuridine(BrdU)-stained samples was performed on paraffin sections of liver tissue (6 μm thick) as described previously (37). Quantification of BrdU-positive nuclei was performed in at least 10 microscope fields (10× objective magnification) in each section using NIS-Elements AR 2.30 (Nikon, Lewisville, TX).…”

Section: Liver Histology Bromodeoxyuridine Staining and Its Quantifimentioning

confidence: 99%

Does Simple Steatosis Affect Liver Regeneration after Partial Hepatectomy in Rats?

Garnol

Kučera

Staňková

et al. 2016

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary:Aim: The aim of our study was to assess whether simple steatosis impairs liver regeneration after partial hepatectomy (PHx) in rats. Methods: Male Sprague-Dawley rats were fed a standard diet (ST-1, 10% kcal fat) and high-fat diet (HFD, 71% kcal fat) for 6 weeks. Then the rats were submitted to 2/3 PHx and animals were sacrificed 24, 48 or 72 h after PHx. Serum biochemistry, respiration of mitochondria in liver homogenate, hepatic oxidative stress markers, selected cytokines and DNA content were measured, and histopathological samples were prepared. Liver regeneration was evaluated by incorporation of bromodeoxyuridine (BrdU) to hepatocyte DNA. Results: HFD induced simple microvesicular liver steatosis. PHx caused elevation of serum markers of liver injury in both groups; however, an increase in these parameters was delayed in HFD group. Hepatic content of reduced glutathione was significantly increased in both groups after PHx. There were no significant changes in activities of respiratory complexes I and II (state 3). Relative and absolute liver weights, total DNA content, and DNA synthesis exerted very similar changes in both ST-1 and HFD groups after PHx. Conclusion: PHx-induced regeneration of the rat liver with simple steatosis was not significantly affected when compared to the lean liver.

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Section: Liver Histology Bromodeoxyuridine Staining and Its Quantifimentioning

confidence: 99%

Does Simple Steatosis Affect Liver Regeneration after Partial Hepatectomy in Rats?

Garnol

Kučera

Staňková

et al. 2016

Acta Med. (Hradec Kralove, Czech Repub.)

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“…Rankin et al found that Hif-2 regulated hepatic EPO expression in the early postnatal, Vhlh-deficient, and anemic adult liver [46]. Most recently, a growing body of evidence suggests that the beneficial effect of EPO treatment on liver resection and living donor liver transplantation in rats [14,[17][18]. Moreover, microarray analysis is a powerful approach for monitoring molecular events during liver regeneration after PH, which has been demonstrated in previous studies [24,25,[33][34][35][36][37][38][39][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, EPO has also been recognized as a pleiotropic cytokine associated with cell proliferation, differentiation, anti-apoptosis, and tissue protection [10][11][12][13]. The beneficial effect of EPO treatment on liver resection and living donor liver transplantation in rats has been demonstrated [14][15][16][17][18][19][20]. Indeed, increased synthesis of EPO has been found in liver regeneration, and enhanced EPO serum levels correlate with the peak of liver regeneration after PH [21].…”

Section: Introductionmentioning

confidence: 99%

Expression profiles uncover the relationship between erythropoietin and cell proliferation in rat hepatocytes after a partial hepatectomy

Zhang

Yang

et al. 2014

Cellular and Molecular Biology Letters

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Erythropoietin (EPO) has a beneficial effect on hepatic cell proliferation during liver regeneration. However, the underlying mechanism has not yet been elucidated. To uncover the proliferation response of EPO in rat liver regeneration after partial hepatectomy (PH) at the cellular level, hepatocytes (HCs) were isolated using Percoll density gradient centrifugation. The genes of the EPO-mediated signaling pathway and the target genes of the transcription factor (TF) in the pathway were identified in a pathway and TF database search. Their expression profiles were then detected using Rat Genome 230 2.0 Microarray. The results indicated that the EPO-mediated signaling pathway is involved in 19 paths and that 124 genes participate, of which 32 showed significant changes and could be identified as liver regeneration-related genes. In addition, 443 targets regulated by the TFs of the pathway and 60 genes associated with cell proliferation were contained in the array. Subsequently, the synergetic effect of these genes in liver regeneration was analyzed using the E(t) mathematical model based on their expression profiles. The results demonstrated that the E(t) values of paths 3, 8, 12 and 14–17 were significantly strengthened in the progressing phase of liver regeneration through the RAS/MEK/ERK or PI3K/AκT pathways. The synergetic effect of the target genes, in parallel with target-related cell proliferation, was also enhanced 12–72 h after PH, suggesting a potential positive effect of EPO on HC proliferation during rat liver regeneration. These data imply that the EPO receptor may allow EPO to promote HC proliferation through paths 3, 8, 12 and 14–17, mediating the RAS/MEK/ERK and PI3K/AκT pathways in rat liver regeneration after PH.

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“…A meta-analysis of 13 published seric variables, coming from the same experimental setting, tried to provide a numeric evaluation of the Epo efficacy at the same endpoints (Table 1) (Tsompos et al 2015a, Tsompos et al 2015b. Furthermore, several publications addressed trials of other similar molecules of growth factors to which the studied molecule also belongs to (Bader et al 2011, Wang et al 2011. The aim of this experimental study was to examine the effect of Epo on rat model and particularly in a pancreas IR protocol.…”

Section: Introductionmentioning

confidence: 99%

The Acute Effect of Erythropoietin on Glucose Levels During Ischemia Reperfusion Injury in Rats

Tsompos¹,

Panoulis²,

Toutouzas³

et al. 2017

FMI

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Proteomic analysis to display the effect of low doses of erythropoietin on rat liver regeneration

Cited by 17 publications

References 40 publications

Does Simple Steatosis Affect Liver Regeneration after Partial Hepatectomy in Rats?

Does Simple Steatosis Affect Liver Regeneration after Partial Hepatectomy in Rats?

Expression profiles uncover the relationship between erythropoietin and cell proliferation in rat hepatocytes after a partial hepatectomy

The Acute Effect of Erythropoietin on Glucose Levels During Ischemia Reperfusion Injury in Rats

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