Proteomic-Based Platelet Activation-Associated Protein SELP May Be a Novel Biomarker for Coagulation and Prognostic in Essential Thrombocythemia

Abstract

Abnormal platelet activation can lead to thrombosis in essential thrombocythemia (ET) and thus impact patient prognosis. Platelet activation-associated proteins are key molecules for platelet activation. However, it is unclear which proteins are most closely associated with the disease’s prognosis. To determine which platelet activation-related proteins can be employed as ET patient prognosis predictors, we used label-free quantification (LFQ) and parallel reaction monitoring (PRM) technology and first determined the serum proteomic expression levels and the differential proteins of ET patients. Then, based on the IPSET (International Prognostic Score for ET), the differential protein associated with the prognostic score was found. To investigate potential processes affecting prognosis, the connection of this protein with prognostic markers, such as thrombotic history, age, white blood cell count, coagulation factors, and inflammatory factors, were further examined. The levels of platelet activation-related proteins GPIbα, SELP, PF4, MMP1, and FLNA were significantly higher in ET patients, according to LFQ and PRM analyses (p < 0.01). Based on regression analysis of the IPSET prognostic score, it is suggested that the SELP level was positively correlated with the prognostic score and prognostic risk factor analysis (p < 0.05). Further regression analysis of SELP with coagulation factors showed that antithrombin (AT-III) was negatively correlated with SELP levels (p < 0.05). Further regression analysis of the inflammatory factors with AT-III and SELP revealed that IL-10, IL-12P70, and IL-31 were negatively correlated with AT-III and SELP (p < 0.01). Platelet activation pathway-related proteins are expressed more frequently in ET patients, and serum SELP may be a prognostic marker for these individuals by encouraging leukocyte increase and inflammatory factor expression and causing aberrant coagulation.

Super Formula for Soluble C-Type Lectin-Like Receptor 2 × D-Dimer in Patients With Acute Cerebral Infarction

“…Regarding diagnosing platelet activation, there are many proposals such as detecting substance due to platelet activation using a flow cytometry, 19 , 20 platelet aggregation using microscopic findings 21 or spontaneous echocardiographic contrast, 42 microparticles using a flow cytometry or enzyme immunosorbent assay (ELISA), 18 PF4 and β-TG 16 , 17 using ELISA. Human platelets and megakaryocytes highly and almost specifically express a CLEC-2 protein on their cell membrane.…”

“… 23 Although PF4 and β-TG are contained in the α-granule of platelets and are used as classical platelet activation markers, they are easily released upon minimal platelet activation that occurs during sampling and require plasma mixed with citrate. 16 , 17 Furthermore, special techniques are required for blood sampling and sample preparation for PF4 and β-TG measurement, whereas CLEC-2 is a platelet membrane protein, while sCLEC-2 is derived from membrane shedding and microvesicles. sCLEC-2 measurement has several advantages over classical platelet activation markers; its measurement can be used in standard blood collection procedures performed in daily clinical laboratory tests.…”

Identification of crucial modules and genes associated with backfat tissue development by WGCNA in Ningxiang pigs

“…OAZ3 evidently aids in the regulation of polyamine concentration during spermiogenesis and contributes to sperm function and fertility ( Gòdia et al, 2020 ; Sarkar et al, 2022 ). P-selectin ( SELP ) belongs to the selectin proteins family and is primarily expressed in platelets, endothelial cells, and immune cells ( Wang et al, 2023 ). SELP modulates thrombus formation through platelets activation, and a positive correlation has been observed between age and SELP expression in hyperlipidemia and thrombosis related diseases ( Koyama et al, 2003 ; Yeini and Satchi-Fainaro, 2022 ).…”