Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2

Hu, Qi; Wei, Lai; Han, Yang; Lau, Alan F.

doi:10.3892/ijo_00000548

Cited by 12 publications

(2 citation statements)

References 56 publications

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“…Several studies identified its anticancer effects [4, 5]. Other active pharmacological constituents had been confirmed to possess antitumor effects [4, 6–8]. This established a pharmacological basis for TNSE anticancer effects.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Tou Nong San on Transplanted Tumor Growth in Nude Mice

Fang

Wang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Tou Nong San (TNS) is a traditional Chinese medicinal decoction used to treat sores and carbuncles. It contains four herbal drugs and one animal medicine: Radix Astragaliseu Seu Hedysari, Angelica sinensis, Ligustici Chuanxiong, Spina Gleditsiae, and stir-baked Squama Manis. Previous studies have shown that it has anticancer effects. This report validates in vivo antitumor properties of TNS. The compounds contained in TNSE were confirmed by liquid chromatographmass spectrometer (LC-MS) analysis. The in vivo antitumor activity of TNS extract (TNSE) was tested by feeding it to athymic mice harboring a human colonic tumor subcutaneous xenograft. Toxicity was monitored by recording behavior and weight parameters. Seven compounds were detected in TNSE by LC-MS. TNSE was fed to athymic mice for 2 weeks. No adverse reactions were reported. Compared to the control group, administration of TNSE to tumor bearing mice significantly reduced both tumor weight and volume. The expressions of p-PI3K, p-AKT, p-mTOR, p-p70s6k1, VEGF, and CD31 were significantly reduced, the expression levels of cleaved Caspase-9 and cleaved Caspase-3 were significantly increased in the TNSE groups compared to the control group as determined by western blot and immunohistochemistry. TNSE produced anticolonic cancer effects and the underlying mechanisms involved inhibition of the PI3K/AKT signal transduction pathway, inhibition of angiogenesis, and promotion of apoptotic proteins.

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Section: Discussionmentioning

confidence: 99%

The Effect of Tou Nong San on Transplanted Tumor Growth in Nude Mice

Fang

Wang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…There have been determined the Saponins, flavonoids, and polysaccharides compounds, which are beneficial to human health, have been determined in Astragalus [18,24,29]. Many species have been used in traditional medicine for their immunostimulant, anti-inflammatory, antioxidative, cardioprotective, hepatoprotective, antiviral properties and ability to treat diabetes [14,15,17,20,22,26,30,31,34,36,38]. Astragalus mongholicus Bunge is a plant with rare status, which is important for sand setting, soil strengthening, and bee pastures during flowering.…”

Section: Introductionmentioning

confidence: 99%

Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge

Munkhbayar,

Gachmaa,

Jukov

et al. 2023

Proc. Mong. Acad. Sci.

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In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin and polysaccharide content in ethanol, methanol and aqueous extracts of different samples were determined by using spectrophotometric methods to investigate whether the content of biologically active compounds depends on the stage of development of the plant during in vitro culture. The content of total saponins and polysaccharides in different cultures of A. mongholicus grown in vitro was higher (990 and 505 μg/ml) in ethanol extracted 14-day-old young shoot samples than in 28-day-old propagated shoot samples and rooted shoots.

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Induction of apoptosis by in vitro and in vivo plant extracts derived from Menyanthes trifoliata L. in human cancer cells

et al. 2019

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Menyanthes trifoliata L. has been used in traditional medicine for centuries. It exists in Asia, Europe, North America and in Morocco and is exploited as a remedy for anemia and lack of appetite. This plant shows many pharmacological properties, but its most interesting one is its anti-cancer potential. The present study examines the induction of apoptosis in grade IV glioma cells after treatment with the extracts from aerial part and root of M. trifoliata plants derived from in vitro (MtAPV and MtRV, respectively) and from soil (MtAPS and MtRS, respectively) and presents the first comparison of the biological effects of four different extracts of M. trifoliata against glioblastoma cells. The root extracts of M. trifoliata plants were found to exhibit cytotoxic effects against grade IV glioma cells, but not normal human astrocytes. HPLC analysis demonstrated the presence of various polyphenolic compounds, including sinapinic acid, ferulic acid, syringic acid and vanilic acid. Higher amount of pentacyclic triterpene (betulinic acid) was also found in MtRV extract. The growth inhibition of human grade IV glioma cells mediated by MtRV extract appears to be associated with apoptosis and G2/M phase cell cycle arrest, and altered expression of the pro- and anti-apoptotic genes ( Bax , Bcl - 2 , Cas - 3 and TP53 ) and proteins (Bax, Bcl-2, Cas-3 and p53), as well as decreased mitochondrial membrane potential. Our results indicate that M. trifoliata gives promising results as an anti-cancer agent for human glioblastoma cell lines. However, further research is necessary in view of its therapeutic use.

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Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2

Cited by 12 publications

References 56 publications

The Effect of Tou Nong San on Transplanted Tumor Growth in Nude Mice

The Effect of Tou Nong San on Transplanted Tumor Growth in Nude Mice

Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge

Induction of apoptosis by in vitro and in vivo plant extracts derived from Menyanthes trifoliata L. in human cancer cells

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