Proteomic Data in Morphine Addiction Versus Real Protein Activity: Metabolic Enzymes

Antolak, Anna; Bodzoń‐Kułakowska, Anna; Cetnarska, Ewa; Pietruszka, Monika; Marszałek‐Grabska, Marta; Kotlińska, Jolanta; Suder, Piotr

doi:10.1002/jcb.26085

Cited by 11 publications

(10 citation statements)

References 45 publications

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“…SOD abundance (lower in maternal than in fetal part) in fifth month matched the activity of this enzyme detected in homogenates of bovine placenta collected around 2 weeks before expected parturition . This observation is particularly important because protein expression, typically measured by proteomics, is not always followed by its enzymatic activity . Proteins mentioned above, such as SOD, were also detected in term bovine placenta …”

Section: Discussionmentioning

confidence: 70%

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2D Electrophoretic pattern of bovine placental proteins during early‐mid pregnancy

et al. 2020

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The Placenta, like every tissue, possesses its own characteristic protein profile, which may change within the course of pregnancy. These changes can be used for the elucidation of the mechanisms related to both physiology of pregnancy and pathological events. The aim of the study was to describe proteinergic profiles of maternal and fetal parts of bovine placenta during early‐mid pregnancy by the use of 2D electrophoresis and MALDI TOF/TOF MS identification to evaluate dynamics of the possible changes necessary for placentation. Placental samples were collected from six pregnant cows (3‐5 months) in the local abattoir. Placentomes were separated, and proteins were extracted and subjected to 2D electrophoresis and MALDI TOF/TOF identification. Out of 907 spots identified by the statistical analysis of gels, 54 were identified. Out of this number, 36 spots were significantly different between examined samples. Moreover, the obtained patterns differed between maternal and fetal parts of the placenta with regard to the intensity of staining, suggesting quantitative differences in protein content. These preliminary results are unique for this period of pregnancy. Such data are important for further experiments to obtain full protein profiles necessary to understand biochemical mechanisms underlying the attachment between fetal and maternal parts of the placenta during placentation. Moreover, the outcomes may help in elucidating pregnancy biomarkers in the future.

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Section: Discussionmentioning

confidence: 70%

“…expression, typically measured by proteomics, is not always followed by its enzymatic activity. 15,16 Proteins mentioned above, such as SOD, were also detected in term bovine placenta. 17 Calreticulin, identified in the present study, may act as a modulator of gene transcription via the nuclear glucocorticoid receptor.…”

Section: Resultsmentioning

confidence: 89%

2D Electrophoretic pattern of bovine placental proteins during early‐mid pregnancy

et al. 2020

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“…2016; Antolak et al. 2017). Many efforts have been made to explore the molecular basis of morphine addiction and some molecules and cellular biological processes have been found.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway

Gao

2019

Pharmaceutical Biology

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Context: Morphine is an alkaloid isolated from the poppy plants. The addiction of morphine is a very serious social issue. Some long non-coding RNAs (lncRNAs) have been proposed to engage in drug addiction. Objective: Whether lncRNA maternally expressed gene 3 (MEG3) attended to morphine-mediated autophagy of mouse hippocampal neuronal HT22 cells was probed. Materials and methods: HT22 cells were subjected to 10 µM morphine for 24 h. Cell autophagy was assessed by measuring LC3-II/LC3-I and Beclin-1 expression. qRT-PCR was carried out to measure MEG3 expression. SiRNA oligoribonucleotides targeting MEG3 (si-MEG3) was transfected to silence MEG3. The orexin1 receptor (OX1R), c-fos, p/t-ERK and p/t-PKC expressions were tested by western blotting. SCH772984 was used as an inhibitor of ERK pathway. Results: Morphine elevated OX1R (2.92 times), c-fos (2.06 times), p/t-ERK (2.04 times) and p/t-PKC (2.4 times), Beclin-1 (3.2 times) and LC3-II/LC3-I (3.96 times) expression in HT22 cells. Moreover, followed by morphine exposure, the MEG3 expression was also elevated in HT22 cells (3.03 times). The silence of MEG3 lowered the Beclin-1 (1.85 times), LC3-II/LC3-I (2.12 times), c-fos (1.39 times) and p/t-ERK (1.44 times) expressions in morphine-treated HT22 cells. Inhibitor of ERK pathway SCH772984 further promoted the influence of MEG3 silence on morphine-caused Beclin-1 (1.97 times) and LC3-II/LC3-I (1.92 times) expressions decreases. Conclusions: Up-regulation of MEG3 attended to the morphine-caused autophagy of HT22 cells might be through elevating c-fos expression and promoting ERK pathway activation. More experiments are also needed in the future to analyse the influence of other lncRNAs in drug addiction.

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“…This database is continuously updated. More than 20% of proteins distinguished in this database are functionally related to regulation of energy metabolism-glycolysis, gluconeogenesis, Krebs cycle and oxidative phosphorylation [35].…”

Section: Plos Onementioning

confidence: 99%

Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal

et al. 2020

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Opioid addiction is recognized as a chronic relapsing brain disease resulting from repeated exposure to opioid drugs. Cellular and molecular mechanisms underlying the ability of organism to return back to the physiological norm after cessation of drug supply are not fully understood. The aim of this work was to extend our previous studies of morphine-induced alteration of rat forebrain cortex protein composition to the hippocampus. Rats were exposed to morphine for 10 days and sacrificed 24 h (groups +M10 and −M10) or 20 days after the last dose of morphine (groups +M10/−M20 and −M10/−M20). The six altered proteins (�2-fold) were identified in group (+M10) when compared with group (−M10) by twodimensional fluorescence difference gel electrophoresis (2D-DIGE). The number of differentially expressed proteins was increased to thirteen after 20 days of the drug withdrawal. Noticeably, the altered level of α-synuclein, β-synuclein, α-enolase and glyceraldehyde-3phosphate dehydrogenase (GAPDH) was also determined in both (±M10) and (±M10/ −M20) samples of hippocampus. Immunoblot analysis of 2D gels by specific antibodies oriented against α/β-synucleins and GAPDH confirmed the data obtained by 2D-DIGE analysis. Label-free quantification identified nineteen differentially expressed proteins in group (+M10) when compared with group (−M10). After 20 days of morphine withdrawal (±M10/−-M20), the number of altered proteins was increased to twenty. We conclude that the morphine-induced alteration of protein composition in rat hippocampus after cessation of drug supply proceeds in a different manner when compared with the forebrain cortex. In forebrain cortex, the total number of altered proteins was decreased after 20 days without morphine, whilst in hippocampus, it was increased.

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Proteomic Data in Morphine Addiction Versus Real Protein Activity: Metabolic Enzymes

Cited by 11 publications

References 45 publications

2D Electrophoretic pattern of bovine placental proteins during early‐mid pregnancy

2D Electrophoretic pattern of bovine placental proteins during early‐mid pregnancy

Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway

Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal

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