Proteomic profile and protease activity in the skin mucus of greater amberjack (Seriola dumerili) infected with the ectoparasite Neobenedenia girellae — An immunological approach

Fernández-Montero, Álvaro; Torrecillas, Silvia; Montero, Daniel; Acosta, F.; Prieto‐Álamo, María‐José; Abril, Nieves; Jurado, Juan

doi:10.1016/j.fsi.2021.01.001

Cited by 21 publications

(8 citation statements)

References 52 publications

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“…Two domains (3 and 12) of the NLRP gene were annotated in this work. This gene has already been widely reported in mammalian species it has been expressed in fish samples exposed to infectious agents, such as in the Japanese sole after bacterial infection 46 , and in the mucus of Seriola dumerili parasitized by Neobenedenia girlellae 47 . Similarly, the upregulated expression of IgC MHC I alpha 3 has also been related to exposure of fishes to pathogens, as reported in the transcriptome of specimens of Salmo salar after viral infection 48 .…”

Section: Discussionmentioning

confidence: 88%

First neurotranscriptome of adults Tambaquis (Colossoma macropomum) with characterization and differential expression between males and females

Miranda,

Veneza,

Ferreira

et al. 2024

Sci Rep

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The Tambaqui is one of the most representative Amazon fish species, being highly exploited in fisheries, aquaculture and as a research model. Nonetheless, data about functional genome are still required to evaluate reproductive and nutrition parameters as well as resistance to pathogens. The of next-generation sequencing has allows assessing the transcriptional processes in non-model species by providing comprehensive gene collections to be used as a database in further genomic applications and increased performance of captive populations. In this study, we relied on RNAseq approach to generate the first transcriptome of the telencephalon from adult males and females of Colossoma macropomum, resulting in a reference dataset for future functional studies. We retrieved 896,238 transcripts, including the identification of 267,785 contigs and 203,790 genes. From this total, 91 transcripts were differentially expressed, being 63 and 28 of them positively regulated for females and males, respectively. The functional annotation resulted in a library of 40 candidate genes for females and 20 for males. The functional enrichment classes comprised reproductive processes (GO:0,048,609; GO:0,003,006; GO:0,044,703; GO:0,032,504; GO:0,019,953) being related to sex differentiation (e.g., SAFB) and immune response (e.g., SLC2A6, AHNAK, NLRC3, NLRP3 and IgC MHC I alpha3), thus indicating that the genes in the neurotranscriptome of Tambaqui participate in sex differentiation and homeostasis of captive specimens. These data are useful to design the selection of genes related to sex determination and animal welfare in raising systems of Tambaqui.

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Section: Discussionmentioning

confidence: 88%

First neurotranscriptome of adults Tambaquis (Colossoma macropomum) with characterization and differential expression between males and females

Miranda,

Veneza,

Ferreira

et al. 2024

Sci Rep

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show abstract

“…Concerning changes in skin mucosal proteins relevant to immune response to infection, greater amberjack, Seriola dumerili infected with Neobenedenia girellae (Fernández-Montero et al, 2021) and common carp infected with Ichthyophthirius multifliis (Saleh et al, 2018), showed differential expression of skin mucosal proteins as the potential components in the response to pathogen infections. In amberjack fish, lower differential expressed proteins (1251 DEPs) were identified in infected fish as compared to healthy fish (1377 DEPs).…”

Section: Proteomicsmentioning

confidence: 98%

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

et al. 2021

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Nowadays, fish production through aquaculture and wild capture is escalating due to the higher fish protein demand for human consumption. Thus, the needs for fish products to be improved in scale and quality are becoming increasingly important to meet the nutrition requirements. The negative impacts caused by pathogens infection both in hatchery and grow-out ponds, have resulted in low aquaculture productivity and economic losses for the farmers. Therefore, a systematic study exploring relevant omics datasets through a systems biology approach will enable scientist to describe the complexity and characteristics of interactions in the host-pathogen network, leading to identifying new targets or biomarkers for diseases infection caused by bacteria, viruses, and parasites. The use of different approaches and emerging technologies as a powerful tool in obtaining a new knowledge for sustainable aquaculture production has been increasingly applied by many researchers over the years. In this paper, several omics approaches used to assess the fish immune response were reviewed. A brief description is provided for the omics approaches applicable to aquacultures, such as genomics, transcriptomics, proteomics, and metabolomics. Each tool used are contributes to develop better treatment and prevention strategies to overcome aquaculture disease due to the microbial infections. In genomics and transcriptomics analysis, their parallel approach involving gene expression studies play important role in identifying immune-related genes of the host, response to infectious diseases. While for proteomics and metabolomics approaches, these omics will provide information on the biomarker discovery and understanding the immune-related biosynthetic pathway during pathogen infection. Additionally, examples of the integrative analysis of multi-omics tools required for researchers to better understand the biological systems of aquatic animals especially fish, will also be provided.

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“…[ 186 ], European sea bass [ 98 ], and lumpsucker [ 102 ] have been published, allowing for the possibility of comparative studies to better understand the dynamics of fish mucus. Moreover, the proteomic profile of fish mucus subjected to different types of stress has been studied, such as chronic wounds [ 116 ], bacterial infection [ 187 , 188 ], parasitic infection [ 189 ], artificial stressors [ 190 ], and sample collection [ 191 ]. These types of studies expand our knowledge of proteomic changes associated with immune processes, and they can be a starting point to develop a powerful tool to identify bioindicators of fish welfare and physiological status via non-invasive methods.…”

Section: Omics Techniques As a Promising Tool In Fish Skin Mucus Rese...mentioning

confidence: 99%

Fish Skin Mucus Extracts: An Underexplored Source of Antimicrobial Agents

et al. 2023

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The slow discovery of new antibiotics combined with the alarming emergence of antibiotic-resistant bacteria underscores the need for alternative treatments. In this regard, fish skin mucus has been demonstrated to contain a diverse array of bioactive molecules with antimicrobial properties, including peptides, proteins, and other metabolites. This review aims to provide an overview of the antimicrobial molecules found in fish skin mucus and its reported in vitro antimicrobial capacity against bacteria, fungi, and viruses. Additionally, the different methods of mucus extraction, which can be grouped as aqueous, organic, and acidic extractions, are presented. Finally, omic techniques (genomics, transcriptomics, proteomics, metabolomics, and multiomics) are described as key tools for the identification and isolation of new antimicrobial compounds. Overall, this study provides valuable insight into the potential of fish skin mucus as a promising source for the discovery of new antimicrobial agents.

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Proteomic profile and protease activity in the skin mucus of greater amberjack (Seriola dumerili) infected with the ectoparasite Neobenedenia girellae — An immunological approach

Cited by 21 publications

References 52 publications

First neurotranscriptome of adults Tambaquis (Colossoma macropomum) with characterization and differential expression between males and females

First neurotranscriptome of adults Tambaquis (Colossoma macropomum) with characterization and differential expression between males and females

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

Fish Skin Mucus Extracts: An Underexplored Source of Antimicrobial Agents

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