Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

Abstract

Cd and Zn have been shown to interact antagonistically or synergistically in various plants. In the present study of dwarf polish wheat (DPW)roots, Cd uptake was inhibited by Zn, and Zn uptake was inhibited by Cd, suggesting that Cd and Zn interact antagonistically in this plant. A study of proteomic changes showed that Cd, Zn, and Cd+Zn stresses altered the expression of 206, 303, and 190 proteins respectively. Among these, 53 proteins were altered significantly in response to all these stresses (Cd, Zn, and Cd+Zn), whereas 58, 131, and 47 proteins were altered in response to individual stresses (Cd, Zn, and Cd+Zn, respectively). Sixty-one differentially expressed proteins (DEPs) were induced in response to both Cd and Zn stresses; 33 proteins were induced in response to both Cd and Cd+Zn stresses; and 57 proteins were induced in response to both Zn and Cd+Zn stresses. These results indicate that Cd and Zn induce differential molecular responses, which result in differing interactions of Cd/Zn. A number of proteins that mainly participate in oxidation-reduction and GSH, SAM, and sucrose metabolisms were induced in response to Cd stress, but not Cd+Zn stress. This result indicates that these proteins participate in Zn inhibition of Cd uptake and ultimately cause Zn detoxification of Cd. Meanwhile, a number of proteins that mainly participate in sucrose and organic acid metabolisms and oxidation-reduction were induced in response to Zn stress but not Cd+Zn stress. This result indicates that these proteins participate in Cd inhibition of Zn uptake and ultimately cause the Cd detoxification of Zn. Other proteins induced in response to Cd, Zn, or Cd+Zn stress, participate in ribosome biogenesis, DNA metabolism, and protein folding/modification and may also participate in the differential defense mechanisms.

Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean

“…Redundancy between the annotated proteins and proteins identified from translation of the soybean genome was identified using BLAST [54], and all the identified proteins was list in Additional file 12: S1-S9. Screening criteria for DAPs were as follows: the proteins with a t-test p-value < 0.05 for two compared groups with three replicates, peptides ≥2 and a protein ratio > 1.5 or < 0.67-fold change [55].…”

Cadmium and zinc uptake and translocation in dwarf Polish wheat seedlings as affected by calcium and potassium combination

“…In dwarf Polish wheat (DPW; Triticum polonicum L., AABB, 2n = 4x = 28), we previously observed mutual inhibition of Cd and Zn uptake by each other and that Cd translocation was influenced by Zn. In addition, a number of genes involved in Ca and K ion channels were differentially regulated by Cd, Zn, and Cd+Zn stresses (Wang et al 2016(Wang et al , 2017b. Thus, Ca and K may participate in the Cd/Zn interactions.…”

Ca alleviated Cd-induced toxicity in Salix matsudana by affecting Cd absorption, translocation, subcellular distribution, and chemical forms

“…Plant Cd accumulation and the subsequent trophic transfer in the food chain pose a serious threat to ecosystems and human health [2,3]. Cd affects the growth and development of plants, inhibits photosynthesis and respiration, imbalances water and nutrient absorption [4][5][6][7], and ultimately results in reduced biomass, leading to leaf yellowing, inhibited root growth and even plant death [8][9][10]. Therefore, it is of great importance to better our understanding of the Cd toxicity mechanism in plants [11,12].…”