Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media

Abstract

Recurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a common cause of obstructive sleep apnea syndrome. Aimed at unraveling the differential modulation of proteins in the two pathologies and at understanding the possible pathways involved in their onset, we analyzed the proteomic profile of the adenoids from 14 RAOM and ATH patients by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE coupled with MS allowed us to identify 23 spots with significant (p-value < 0.05) changes in protein amount, recognizing proteins involved in neutrophil degranulation and glycolysis pathways.

Small extracellular vesicles from malignant ascites of patients with advanced ovarian cancer provide insights into the dynamics of the extracellular matrix

“…Samples were next resuspended in 0.1% formic acid/5% acetonitrile and analyzed with LC‐MS/MS (Q‐Trap 6500+), while the raw data files were investigated with the Mascot search engine version v.2.6 (Matrix Science, London, UK). As described in an earlier study [ 27 ], the spectra were searched against the human section of the UniProt database applying the following parameters: Enzyme specificity was set to trypsin with 1 missed cleavage allowed, and precursor and fragment ions tolerance were 0.6 Da. Carboxymethylcysteine and oxidation of methionine were selected as fixed and variable modifications, respectively.…”