Proteomic Study of Plasma from Moderate Hypercholesterolemic Patients

Abstract

Proteomics is a technology to detect and identify several proteins and their isoforms in a single sample. We used proteomics to analyze modifications in the protein map of plasma after simvastatin treatment of moderate hypercholesterolemic patients. Plasma from hypercholesterolemic patients (n = 9) was compared before and after 12 weeks of simvastatin treatment (40 mg/day). Patients with similar cardiovascular risk factors were used as controls (CR group). By using two-dimensional electrophoresis and mass spectrometry, we identified the different protein isoforms. The plasma expression of three fibrinogen gamma chain isoforms (FGG) was enhanced, whereas the expression of two isoforms of the fibrinogen beta chain (FGB) was reduced in the hypercholesterolemic patients compared with the CR group. The expression of apolipoprotein A-IV and three haptoglobin isoforms was higher in hypercholesterolemic patients. Simvastatin treatment modified the plasma expression of FGG chain isoform 1, FGB chain isoforms 1 and 2, vitamin D binding protein isoform 3, apo A-IV, and haptoglobin isoform 2. The modification of FGG chain isoform 1 and FGB chain isoforms 1 and 2 was positively correlated with total plasma cholesterol level. Proteomic analysis of plasma may help to know more in depth the molecular mechanism modified by simvastatin treatment.

Proteomic Approach to the Study of Statin Pleiotropy in Kidney Transplant Patients

“…MS has been previously used in order to shed light on the molecular mechanisms of statin therapy in humans [11][12][13][14] . These studies have provided information about the possible pleiotropic effects but, to our knowledge, this is the first time that the effect of a statin is studied on the peptide profile of plasma from kidney transplant patients.…”

Relationship between Vitamin D Binding Protein and Aspirin Resistance in Coronary Ischemic Patients: A Proteomic Study

“…The peptide matched and the sequence peptides matched for each of the plasma protein and plasma protein isotypes analyzed using mass spectrometry as previously described. 12 TxB2 Determination and Platelet-Rich Plasma Isolation. Whole blood was obtained from seven male healthy volunteers (38 ( 4 years) who, at least for 15 days, had not taken aspirin nor any other antiplatelet or antiinflamatory drugs.…”

“…We recently reported protein expression differences in plasma from patients during an acute coronary syndrome 11 and from moderate hypercholesterolemic patients. 12 This study is the first to use 2DE to compare expression changes in protein and protein isotypes in the plasma of aspirin-resistant to aspirin-sensitive coronary ischemic patients, and it identified several proteins whose expression was increased in aspirinresistant patients. These results may aid future development of more effective therapies for aspirin-resistant patients.…”

Efecto del alprostadil en los cultivos de músculo esquelético isquémico. Estudio proteómico comparativo frente a músculo sano

“…Como se ha publicado con anterioridad (137,138), los geles fueron rehidratados activamente a 50 V durante 60 horas, seguido de rápidas subidas lineales de tensión y voltaje, limitados por una corriente máxima de 50 µA por gel (139).…”