Proteomic tools to study drug function

Mulder, Celine; Leijten, Niels; Lemeer, Simone

doi:10.1016/j.coisb.2018.05.002

Cited by 3 publications

(1 citation statement)

References 83 publications

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“…Mass spectrometry (MS) has become an important tool in the target identification of bioactive compounds, in benefit of its advantages in analyzing complex protein samples [1,2]. In recent years, a variety of modification-free approaches were developed to detect the stability changes of protein in the absence and presence of small ligands, providing an efficient strategy to directly employ compounds without further structural modification [3,4]. The modification-free proteomic technology is not only suitable for the target identification of natural products that are short of modification sites or synthetic unattainable, but also powerful to unbiasedly understanding of the molecular mechanism of a bioactive molecule from a proteome-wide view.…”

Section: Introductionmentioning

confidence: 99%

Solvent‐induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs

Chen

et al. 2023

Proteomics

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Target identification by modification‐free proteomic approaches can potentially reveal the pharmacological mechanism of small molecular compounds. By combining the recent solvent‐induced protein precipitation (SIP) method with TMT‐labeling quantitative proteomics, we propose solvent‐induced proteome profiling (SIPP) approach to identify small molecule–protein interactions. The SIPP approach enables to depict denaturation curves of the target protein by varying concentrations of organic solvents to induce unfolding and precipitation of the cellular proteome. By using this approach, we have successfully identified the known targets of market drugs and natural products and extended the proteome information of SIP for target identification.

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