Proteomics analysis of human oligodendroglioma proteome

Khaghani-Razi-Abad, Solmaz; Hashemi, Mehrdad; Pooladi, Mehdi; Entezari, Maliheh; Kazemi, E.

doi:10.1016/j.gene.2015.05.039

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…The cells in each group were collected, rinsed with PBS, and centrifuged before the addition of lysis buffer to extract the protein. The protein purity was evaluated by the Bradford method . After albuminous degeneration, an equal amount of protein (20 μg) from each group was separated by SDS‐PAGE.…”

Section: Methodsmentioning

confidence: 99%

“…The protein purity was evaluated by the Bradford method. (28) After albuminous degeneration, an equal amount of protein (20 lg) from each group was separated by SDS-PAGE. The separated proteins were transferred onto PDVF membranes and blocked with skim milk powder for 4 h. The primary antibody was added at an appropriate diluted concentration; the blots were incubated overnight, rewarmed for 1 h, and then rinsed with TBS-T three times for 15 min each time.…”

Section: Methodsmentioning

confidence: 99%

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Suberoylanilide hydroxamic acid sensitizes neuroblastoma to paclitaxel by inhibiting thioredoxin‐related protein 14‐mediated autophagy

Zhen¹,

Yang

et al. 2017

Cancer Science

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Paclitaxel is not as effective for neuroblastoma as most of the front‐line chemotherapeutics due to drug resistance. This study explored the regulatory mechanism of paclitaxel‐associated autophagy and potential solutions to paclitaxel resistance in neuroblastoma. The formation of autophagic vesicles was detected by scanning transmission electron microscopy and flow cytometry. The autophagy‐associated proteins were assessed by western blot. Autophagy was induced and the autophagy‐associated proteins LC3‐I, LC3‐II, Beclin 1, and thioredoxin‐related protein 14 (TRP14), were found to be upregulated in neuroblastoma cells that were exposed to paclitaxel. The inhibition of Beclin 1 or TRP14 by siRNA increased the sensitivity of the tumor cells to paclitaxel. In addition, Beclin 1‐mediated autophagy was regulated by TRP14. Furthermore, the TRP14 inhibitor suberoylanilide hydroxamic acid (SAHA) downregulated paclitaxel‐induced autophagy and enhanced the anticancer effects of paclitaxel in normal control cancer cells but not in cells with upregulated Beclin 1 and TRP14 expression. Our findings showed that paclitaxel‐induced autophagy in neuroblastoma cells was regulated by TRP14 and that SAHA could sensitize neuroblastoma cells to paclitaxel by specifically inhibiting TRP14.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Suberoylanilide hydroxamic acid sensitizes neuroblastoma to paclitaxel by inhibiting thioredoxin‐related protein 14‐mediated autophagy

Zhen¹,

Yang

et al. 2017

Cancer Science

View full text Add to dashboard Cite

show abstract

“…A considerable part of the proteins has changed in the malignant astrocytoma tumor after the translation (8); also the membrane proteins were increased and played an important role in concentrating glial cell processes such as G Protein (9) and Epidermal Growth Factor Receptor (EGFP) (10) Ous studies. In continuation, we present our previous studies in more details (1,11). We examined the Folding changes, Molecular Weight (MW) and Isoelectric pH (PI) for a variety of malignant glioma tumors by two-dimensional electrophorese and mass spectroscopy method; such tumors include astrocytoma and oligodendroglia with malignancy grade III and astrocytoma with malignancy grade IV whose changes and comparison was analyzed.…”

Section: Introductionmentioning

confidence: 99%

Characterization of G protein β subunit expression of human brain Glioma tumor

Pooladi¹,

Entezari²,

Hashemi³

2020

BLL

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“…Nevertheless, from the genetic and histopathological perspectives, the underlying mechanisms of breast cancer growth remain mainly unknown. Furthermore, the majority of them are estrogen-dependent diseases with augmenting morbidity and mortality rates (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

The therapeutic effects of Physalis alkekengi hydroalcoholic extract on estrogen receptor-positive breast cancer mice model: possible role of autophagy in this therapeutic response

Zare

Teimouri

2020

J Shahrekord Univ Med Sci

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Background and aims: Although some preclinical and clinical studies have extensively confirmed the pharmacological effects of the hydroalcoholic extract (HE) of Physalis alkekengi on several diseases, little is known about the effects of P. alkekengi HE (PAHE) on breast cancer. Therefore, this study aimed to investigate the therapeutic effect of PAHE on estrogen receptor+ breast cancer. Methods: To this end, tumors were created in mice by injecting MC4L2 cells into the sternum of the mice. Then, the animals were gavaged for 16 days at 10, 50, and 100 mg/kg daily of PAHE. In addition, the tumor growth and body weight of the mice were measured on the 16th day, and they were killed on 21st day. Finally, their tumor tissues were removed and the apoptotic cell tissue and expression of the ATG-5 gene were studied as well. The experiments were repeated three times, and the data were analyzed using SPSS software (P<0.001 and P<0.05). Results: The average body weight of the control group significantly decreased 16 days after tumor establishment (P<0.001). Further, the PAHE inhibited the growth of the breast cancer tumor in higher doses (50 & 100 mg/kg, P<0.001). Based on the results, a significant histopathological alteration was found in the breast tumors of the PAHE-treated groups compared with the control group, including the decreased level of mitotic cells the intensive level of necrotic cells and lymphocyte infiltration into the breast tumors bearing mice 21 days after PAHE administration (P=0.012). Eventually, PAHE significantly increased the mRNA level of the expression of the autophagy ATG-5 specific gene in the effective dosage-treated group (50 mg/kg, P=0.037). Conclusion: The evidence suggests that the PAHE has a suitable efficacy for the treatment of ER+ breast cancer by promoting autophagy mechanisms into these tumor types

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Proteomics analysis of human oligodendroglioma proteome

Cited by 6 publications

References 25 publications

Suberoylanilide hydroxamic acid sensitizes neuroblastoma to paclitaxel by inhibiting thioredoxin‐related protein 14‐mediated autophagy

Suberoylanilide hydroxamic acid sensitizes neuroblastoma to paclitaxel by inhibiting thioredoxin‐related protein 14‐mediated autophagy

Characterization of G protein β subunit expression of human brain Glioma tumor

The therapeutic effects of Physalis alkekengi hydroalcoholic extract on estrogen receptor-positive breast cancer mice model: possible role of autophagy in this therapeutic response

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